BRCA1 CRISPR Plasmids (h) human BRCA1-specific CRISPR/Cas9 KO Plasmid, HDR Plasmid, Double Nickase Plasmids, CRISPR Activation Plasmids and Lentiviral Activation Particles

BRCA1 CRISPR Plasmids (h)

  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 49ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;
Datasheets
    • Target species: human; for corresponding mouse product, see BRCA1 CRISPR Plasmids (m)
    • CRISPR/Cas9 KO Plasmids consists of BRCA1-specific 20 nt guide RNA sequences derived from the GeCKO (v2) library
    • For CRISPR gene knockout, gRNA sequences direct the Cas9 protein to induce a site-specific double strand break (DSB) in the genomic DNA
    • Target-specific CRISPR Plasmids for both gene knockout and activation are available. Please refer to the detailed product information in the tabs below
    • Gene knockdown or activation can be assayed using BRCA1 Antibody (D-9): sc-6954
    • All products are provided as transfection-ready, purified plasmid DNA, except the Lentiviral Activation Particles which have been prepackaged as Lentiviral Particles for use with hard-to-transfect cells
Submit a review for this product and receive 15 CruzCredits

Gene Info

SpeciesGene NameGene IDChromosome LocationIsoform (mRNA) Accession #Protein Accession #OMIM™ Number
HumanBRCA167217q21.31NM_007294, NM_007297, NM_007298, NM_007299, NM_007300, NM_007295, NM_007296, NM_007301, NM_007302, NM_007303, NM_007305, NM_007306P38398113705

We just bought the BRCA1 CRISPR/Cas9 KO (h) plasmid. However, the transfection efficiency is only ~1%. We need do some trouble shoots. Do you have the plasmid sequence available? What is the antibiotic selection? Thanks advance for your answer.

Asked by: yangcs01
Thank you for your question. Please contact our technical support group by phone, (800)-457-3801 option 2, email <scbt@scbt.com>, or by live chat directly on our website, www.scbt.com, and we would be happy to provide you with this information and help you troubleshoot the CRISPR.
Answered by: Tech Service
Date published: 2017-08-14
  • y_2019, m_6, d_24, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.12
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_en_US, sid_400093, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 40ms
  • QUESTIONS, PRODUCT
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 205ms
  • REVIEWS, PRODUCT
  • bvseo-msg: The resource to the URL or file is currently unavailable.;

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.