Pituitary ExtractWestern blotting positive control

Pituitary Extract

Bovine Pituitary Extract (BPE) is rated 5.0 out of 5 by 1.
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Application: A mitogenic supplement in serum-free growth medium
* Refer to Certificate of Analysis for lot specific data (including water content).
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Pituitary Extract is usually used as a mitogenic supplement in serum-free growth medium. Studies suggest that this compound contains growth factors required for proliferation of melanocytes. Additional experiments show that, in addition to its mitogenic activity, It displays antioxidant characteristics. Pituitary Extract has been reported to provide protection against H2O2-induced cell death, protein oxidation, membrane fragmentation, and deoxyribonucleic acid damage.


References

1. Hirobe, T. 1991. J. Exp. Zool. 257: 184-194. PMID: 1990051
2. Kent, K.D. and Bomser, J.A. 2003. In Vitro Cell. Dev. Biol. Anim. 39: 388-394. PMID: 14690452

Usage :
Growth tests on human mammary epithelial cells, epidermal keratinocytes, prostatic and bronchial epithelial cells indicate that the optimal medium supplementation rate is 35 μg/mL. However, because this product is a complex mixture of factors, different cell types may require adjusted supplementation levels. A simple growth assay, titrating Pituitary Extract into your system, may be useful.
Formulation :
~14 mg/mL crude protein
Physical State :
Liquid
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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Certificate of Analysis

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Rated 5 out of 5 by from It played well! BPE is used as a mitogenic supplement in serum-free growth medium.contains growth factors required for proliferation of melanocytes. It played well in the cell culture.
Date published: 2016-11-25
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