Blasticidin S Hydrochloride An antibiotic inhibiting protein synthesis

Blasticidin S Hydrochloride (CAS 3513-03-9)

Blasticidin S Hydrochloride | CAS 3513-03-9 is rated 5.0 out of 5 by 1.
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Application: An antibiotic inhibiting protein synthesis
CAS Number: 3513-03-9
Purity: ≥98%
Molecular Weight: 458.9
Molecular Formula: C17H26N8O5•HCl
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Blasticidin S Hydrochloride, like puromycin (sc-205821), belongs to a group of cytosine amino nucleoside antibiotics. Blasticidin S is isolated from Streptomyces griseochromogenes and inhibits protein synthesis in both prokaryotic and eukaryotic cells, it acts quickly by inhibiting peptide bond formation by the ribosome.

The blasticidin S resistance gene (BSR), found in Bacillus cereus K55-S1, has been used in multiple studies for its use as a selective compound. Resistance is conferred by expression of one of two Blasticidin S deaminase genes: BSD from Aspergillus terreus or bsr from Bacillus cereus. These deaminases convert Blasticidin S to a nontoxic deaminohydroxy derivative.


References

1. Takeuchi, S., et al. 1958. J. Antibiot. 11: 1-5. PMID: 13525246
2. Swaminathan, V., et al. 1981. Biochim. Biophys. Acta. 655: 335-341. PMID: 7284390
3. Itaya, M., et al. 1990. J. Biochem. 107: 799-801. PMID: 2118136
4. Kamakura, T., et al. 1990. Mol. Gen. Genet. 223: 332-334. PMID: 2250657
5. Izumi, M., et al. 1991. Exp. Cell Res. 197: 229-233. PMID: 1720391
6. Kimura, M., et al. 1994. Biochim. Biophys. Acta. 1219: 653-659. PMID: 7948022
7. Kimura, M., et al. 1994. Mol. Gen. Genet. 242: 121-129. PMID: 8159161
8. Nishimura, M., et al. 2006. J. Biosci. Bioeng. 101: 63-69. PMID: 16503293

Usage :
Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 1–20 µg/mL depending on the cell line.


Blasticidin S may also be used as a bacterial selection antibiotic in the concentration range of 50–100 µg/mL. To maintain the activity of Blasticidin S for selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0.

Appearance :
Powder
Physical State :
Solid
Derived From :
Stretomyces girseochromogenes
Solubility :
Soluble in water. Insoluble in organic solvents.
Storage :
Store at 4° C
Melting Point :
218.0-225.0° C
Boiling Point :
772.1° C at 760 mmHg (Predicted)
Refractive Index :
n20D ~1.71 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
25078183
Merck Index :
14: 1316
MDL Number :
MFCD02091640
EC Number :
264-899-3
SMILES :
CN(CC[C@H](CC(=O)N[C@H]1C=C[C@@H](O[C@@H]1C(=O)O)N2C=CC(=NC2=O)N)N)C(=N)N.Cl

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Certificate of Analysis

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Blasticidin S Hydrochloride  Product Citations

See how others have used Blasticidin S Hydrochloride. Click on the entry to view the PubMed entry .

Citations 1 to 5 of 5 total

PMID: # 28864500  Liu, Y. et al. 2017. Am. J. Physiol. Gastrointest. Liver Physiol. 313: G478-G491.

PMID: # 28065601  Juszkiewicz, S. et al. 2016. Mol. Cell.

PMID: # 26391343  Granato, M. et al. 2015. Autophagy. 11: 1978-1986.

PMID: # 26217182  Adragna, NC. et al. 2015. Frontiers in cellular neuroscience. 9: 255.

PMID: # 16476734  Maeda, K. et al. 2006. J. Biol. Chem. 281: 12688-12698.

Citations 1 to 5 of 5 total
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Rated 5 out of 5 by from This chemical has been working well This chemical has been working well. Very satisfied with purity.
Date published: 2015-04-08
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