BIS-Tris Propane is a non-zwitterionic buffer that is useful in the pH 6.3 - 9.5 range. This wide buffering range is due to its two pKa values, 6.8 (pKa1) and 9.0 (pKa2), being so close. Because of the wide buffering range, particularly down to pH 6 - 7, this buffer has been used to enhance the stability or activity of restriction enzymes, compared to Tris buffer (which is a poor buffer below pH 7.5 and has a comparatively large change in pKa with temperature).
Eckert, K. A., and Kunkel, T. A., DNA polymerase fidelity and the polymerase chain reaction. PCR Methods Appl., 1(1), 17-24 (1991).
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