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Bicine (cell culture suitable) (CAS 150-25-4)

Synonym: N,N-Bis-(2-hydroxyethyl)-glycine

Application: A zwitterionic amino acid buffer agent used for low temperature work

CAS Number: 150-25-4

Molecular Weight: 163.17

Molecular Formula: C₆H₁₃NO₄

* Refer to Certificate of Analysis for lot specific data (including water content).

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SDS & Certificate of Analysis

Bicine is a zwitterionic amino acid buffer, active in the pH 7.6-9.0 range (pK_a of 8.26 at 25°C). Recommended buffer for low temperature biochemical work. Bicine is used for the preparation of stable substrate solution for serum guanase determination. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

Appearance :

Powder

Physical State :

Solid

Solubility :

Soluble in water (163.17 g/l) at 20 °C, ethanol (slightly soluble), ether (slightly soluble), and chloroform (slightly soluble).

Storage :

Store at room temperature

Melting Point :

190 °C (dec.)

Boiling Point :

361.50-415.50 °C at 760 mmHg (Predicted)

Density :

1.31 g/cm³ (Predicted)

Refractive Index :

n²⁰_D 1.53 (Predicted)

pK Values :

pK_a: 8.3 (0.1 M, 25 °C)

For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

WGK Germany :

RTECS :

MB9700000

PubChem CID :

8761

Merck Index :

14: 1201

MDL Number :

MFCD00004295

EC Number :

205-755-1

Beilstein Registry :

1769362

SMILES :

C(CO)N(CCO)CC(=O)O

Download SDS (MSDS)

Certificate of Analysis

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