Bicine (cell culture suitable) A zwitterionic amino acid buffer agent used for low temperature work

Bicine (cell culture suitable) (CAS 150-25-4)

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Synonym: N,N-Bis-(2-hydroxyethyl)-glycine
Application: A zwitterionic amino acid buffer agent used for low temperature work
CAS Number: 150-25-4
Molecular Weight: 163.17
Molecular Formula: C6H13NO4
* Refer to Certificate of Analysis for lot specific data (including water content).
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Bicine is a zwitterionic amino acid buffer, active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Recommended buffer for low temperature biochemical work. Bicine is used for the preparation of stable substrate solution for serum guanase determination. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (163.17 g/l) at 20 °C, ethanol (slightly soluble), ether (slightly soluble), and chloroform (slightly soluble).
Storage :
Store at room temperature
Melting Point :
190 °C (dec.)
Boiling Point :
361.50-415.50 °C at 760 mmHg (Predicted)
Density :
1.31 g/cm3 (Predicted)
Refractive Index :
n20D 1.53 (Predicted)
pK Values :
pKa: 8.3 (0.1 M, 25 °C)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
MB9700000
PubChem CID :
8761
Merck Index :
14: 1201
MDL Number :
MFCD00004295
EC Number :
205-755-1
Beilstein Registry :
1769362
SMILES :
C(CO)N(CCO)CC(=O)O

Download SDS (MSDS)

Certificate of Analysis

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