Bicine is a zwitterionic amino acid buffer, active in the pH 7.6-9.0 range (pKa of 8.26 at 25°C). Recommended buffer for low temperature biochemical work. Bicine is used for the preparation of stable substrate solution for serum guanase determination. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.
1. D.L. Williams-Smith Biochem. J. 167, 593, (1977) 2. S. Ito Clin. Chim. Acta 115, 135, (1981) 3. M. Bier, A. Kopwillem Electrochim. Acta Berlin, Ger. , 567, (1976)
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