beta-Actin Antibody (C4)
  • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
  • raised against gizzard Actin of chicken origin
  • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
  • available conjugated to agarose (sc-47778 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-47778 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-47778 PE), fluorescein (sc-47778 FITC), Alexa Fluor® 488 (sc-47778 AF488) or Alexa Fluor® 647 (sc-47778 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • also available conjugated to biotin (sc-47778 B, 200 µg/ml) for WB, IHC(P) and ELISA
  • TransCruz reagent for ChIP application (sc-47778 X, 200 µg/0.1 ml)
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

β-Actin Antibody (C4): sc-47778

beta-Actin Antibody (C4) is rated 4.9 out of 5 by 204.
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Datasheets
    • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against gizzard Actin of chicken origin
    • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • available conjugated to agarose (sc-47778 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-47778 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-47778 PE), fluorescein (sc-47778 FITC), Alexa Fluor® 488 (sc-47778 AF488) or Alexa Fluor® 647 (sc-47778 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • also available conjugated to biotin (sc-47778 B, 200 µg/ml) for WB, IHC(P) and ELISA
    • TransCruz reagent for ChIP application (sc-47778 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
β-Actin Antibody (C4) sc-47778 200 µg/ml $279.00
β-Actin Antibody (C4) AC sc-47778 AC 500 µg/ml, 25% ag $366.00
β-Actin Antibody (C4) Alexa Fluor® 488 sc-47778 AF488 200 µg/ml $314.00
β-Actin Antibody (C4) Alexa Fluor® 647 sc-47778 AF647 200 µg/ml $314.00
β-Actin Antibody (C4) B sc-47778 B 200 µg/ml $282.00
β-Actin Antibody (C4) FITC sc-47778 FITC 200 µg/ml $292.00
β-Actin Antibody (C4) HRP sc-47778 HRP 200 µg/ml $279.00
β-Actin Antibody (C4) PE sc-47778 PE 200 µg/ml $303.00
β-Actin Antibody (C4) X sc-47778 X 200 µg/0.1 ml $279.00

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beta-Actin Antibody (C4) Product Citations

See how others have used beta-Actin Antibody (C4). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 1369 total

PMID: # 27845687
Le Dour, C. et al. 2017. Extracellular matrix remodeling and transforming growth factor-β signaling abnormalities induced by lamin A/C variants that cause lipodystrophy. J. Lipid Res.. 58: 151-163.

PMID: # 27922685
Yu, GQ. et al. 2017. 5-bromo-3-(3-hydroxyprop-1-ynyl)-2H-pyran-2-one induces apoptosis in T24 human bladder cancer cells through mitochondria-dependent signaling pathways. Mol Med Rep. 15: 153-159.

PMID: # 27717813
Ha, S. et al. 2017. Human adipose-derived stem cells ameliorate repetitive behavior, social deficit and anxiety in a VPA-induced autism mouse model. Behav. Brain Res.. 317: 479-484.

PMID: # 28061449
Wolter, P. et al. 2017. Central spindle proteins and mitotic kinesins are direct transcriptional targets of MuvB, B-MYB and FOXM1 in breast cancer cell lines and are potential targets for therapy. Oncotarget.

PMID: # 27381181
Tahrir, FG. et al. 2017. Evidence for the Role of BAG3 in Mitochondrial Quality Control in Cardiomyocytes. J. Cell. Physiol.. 232: 797-805.

PMID: # 27884681
Kim, JY. et al. 2017. Acidic stress induced G1 cell cycle arrest and intrinsic apoptotic pathway in Jurkat T-lymphocytes. Exp. Cell Res.. 350: 140-146.

PMID: # 28098159
Lee, HJ. et al. 2017. Fluid shear stress activates YAP1 to promote cancer cell motility. Nature communications. 8: 14122.

PMID: # 27986811
Li, JJ. et al. 2017. EphB3 Stimulates Cell Migration and Metastasis in a Kinase-dependent Manner through Vav2-Rho GTPase Axis in Papillary Thyroid Cancer. J. Biol. Chem.. 292: 1112-1121.

PMID: # 28133573
Ha, SH. et al. 2017. Jellyfish extract induces apoptotic cell death through the p38 pathway and cell cycle arrest in chronic myelogenous leukemia K562 cells. PeerJ. 5: e2895.

PMID: # 27864075
Biedenkopf, N. et al. 2017. The natural compound silvestrol is a potent inhibitor of Ebola virus replication. Antiviral Res.. 137: 76-81.

Citations 1 to 10 of 1369 total

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: cjMara
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2016-12-13

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: cjMara
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, β-Actin Antibody (C4): sc-47778, is currently available conjugated to HRP (sc-47778 HRP), phycoerythrin (sc-47778 PE), fluorescein (sc-47778 FITC), Alexa Fluor® 488 (sc-47778 AF488), Alexa Fluor® 647 (sc-47778 AF647), and biotin (sc-47778 B).
Answered by: Technical Support
Date published: 2016-12-13

I am using sc-47778 , but i am getting many bands at different M.W., what can the problem be ?

Asked by: Soumya Ganguly
Thank you for your question. That is a very surprising result, as this is one of our most popular loading control antibodies against Beta Actin. Non-specific binding can result from different variables and there are a lot of different options for troubleshooting. We recommend blocking and diluting your primary and secondary antibodies in 5% nonfat milk + TBST. We also recommend using an appropriate secondary antibody from SCBT, and including a secondary control (incubated without primary antibody) to try and identify if the non-specific bands are due to your secondary antibody alone. I am providing a link below to our recommended protocol for Western blotting. If you still need help, please contact our Technical Service Department by phone (our Asian office toll-free number is 800-820-8626), by e-mail, asia@scbio.cn, or by live chat. https://www3.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2016-09-22
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Rated 5 out of 5 by from perfect loading control antibody Our lab use this antibody for WB loading control over years.always strong and clean bands at 43kda.
Date published: 2017-02-23
Rated 5 out of 5 by from Excellent antibody! I used this antibody for the WB for the protein extracted from H9C2 cells,the band is special and clear!
Date published: 2017-02-15
Rated 5 out of 5 by from 43kda always This antibody is good quality control antibody for IHC and WB,strong band and clear staining always.
Date published: 2017-02-12
Rated 5 out of 5 by from  beautiful and perfect! The antibodies only use 1 ul I very clean and beautiful, it is very clean, beautiful and perfect!
Date published: 2017-02-11
Rated 5 out of 5 by from Used it for many years I used this antibody for many years, never meet problems, 1:2000 to 1:4000 usd for WB.
Date published: 2017-02-11
Rated 5 out of 5 by from good antibody The antibody worked well for WB on the A549 cells lysate with the dilution of 1:200.
Date published: 2017-02-10
Rated 5 out of 5 by from Valuable antibodies We usually use it to detect p - JNK mouse, we usually use diluted 1:200 can get the desired results
Date published: 2017-02-09
Rated 5 out of 5 by from very good quality loading control. I recommend use this antibody for loading control ,always strong band in WB, 1:3000 been diluted.
Date published: 2017-02-09
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