beta-Actin Antibody (C4)
  • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
  • raised against gizzard Actin of avian origin
  • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
  • available conjugated to agarose (sc-47778 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-47778 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-47778 PE), fluorescein (sc-47778 FITC), Alexa Fluor® 488 (sc-47778 AF488) or Alexa Fluor® 647 (sc-47778 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • also available conjugated to biotin (sc-47778 B, 200 µg/ml) for WB, IHC(P) and ELISA
  • available as TransCruz reagent for ChIP application (sc-47778 X, 200 µg/0.1 ml)
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, m-IgGκ BP-FITC: sc-516140, m-IgGκ BP-PE: sc-516141, or m-IgGκ BP-B: sc-516142, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

β-Actin Antibody (C4): sc-47778

beta-Actin Antibody (C4) is rated 4.777777777777778 out of 5 by 90.
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Datasheets
    • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against gizzard Actin of avian origin
    • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • available conjugated to agarose (sc-47778 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-47778 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-47778 PE), fluorescein (sc-47778 FITC), Alexa Fluor® 488 (sc-47778 AF488) or Alexa Fluor® 647 (sc-47778 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • also available conjugated to biotin (sc-47778 B, 200 µg/ml) for WB, IHC(P) and ELISA
    • available as TransCruz reagent for ChIP application (sc-47778 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, m-IgGκ BP-FITC: sc-516140, m-IgGκ BP-PE: sc-516141, or m-IgGκ BP-B: sc-516142, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
β-Actin Antibody (C4) sc-47778 200 µg/ml $279.00
β-Actin Antibody (C4) AC sc-47778 AC 500 µg/ml, 25% ag $366.00
β-Actin Antibody (C4) Alexa Fluor® 488 sc-47778 AF488 200 µg/ml $314.00
β-Actin Antibody (C4) Alexa Fluor® 647 sc-47778 AF647 200 µg/ml $314.00
β-Actin Antibody (C4) B sc-47778 B 200 µg/ml $282.00
β-Actin Antibody (C4) FITC sc-47778 FITC 200 µg/ml $292.00
β-Actin Antibody (C4) HRP sc-47778 HRP 200 µg/ml $279.00
β-Actin Antibody (C4) PE sc-47778 PE 200 µg/ml $303.00
β-Actin Antibody (C4) X sc-47778 X 200 µg/0.1 ml $279.00

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beta-Actin Antibody (C4) Product Citations

See how others have used beta-Actin Antibody (C4). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 1121 total

PMID: # 26962121
Wang, XJ. et al. 2016. Dibutyl Phthalate Inhibits the Effects of Follicle-Stimulating Hormone on Rat Granulosa Cells Through Down-Regulation of Follicle-Stimulating Hormone Receptor. Biology of reproduction.

PMID: # 26358154
Charni, M. et al. 2016. Novel p53 target genes secreted by the liver are involved in non-cell-autonomous regulation. Cell death and differentiation. 23: 509-20.

PMID: # 26845565
Harter, M.R. et al. 2016. BS69/ZMYND11 C-Terminal Domains Bind and Inhibit EBNA2. PLoS pathogens. 12: e1005414.

PMID: # 26906715
Lee, S. et al. 2016. IK-guided PP2A suppresses Aurora B activity in the interphase of tumor cells. Cellular and molecular life sciences : CMLS.

PMID: # 26607113
Kim, SR. et al. 2016. H3S10 phosphorylation-mediated transcriptional regulation by Aurora kinase A. Biochem. Biophys. Res. Commun.. 469: 22-8.

PMID: # 26893726
Chang, L. et al. 2016. Combined RNAi targeting human Stat3 and ADAM9 as gene therapy for non-small cell lung cancer. Oncol Lett. 11: 1242-1250.

PMID: # 26896429
Zhu, Y. et al. 2016. HOXA10, EMX2 and TENM1 expression in the mid-secretory endometrium of infertile women with a Müllerian duct anomaly. Reprod. Biomed. Online. 32: 388-93.

PMID: # 26997968
Garcia, RA. et al. 2016. Evaluation of markers of beige adipocytes in white adipose tissue of the mouse. Nutrition & metabolism. 13: 24.

PMID: # 26122028
Togliatto, G. et al. 2016. Obesity reduces the pro-angiogenic potential of adipose tissue stem cell-derived extracellular vesicles (EVs) by impairing miR-126 content: impact on clinical applications. International journal of obesity (2005). 40: 102-11.

PMID: # 26511067
Krone, KG. et al. 2016. Interaction of vitamin A supplementation level with ADH1C genotype on intramuscular fat in beef steers. Animal. 10: 403-9.

Citations 1 to 10 of 1121 total

I am using sc-47778 , but i am getting many bands at different M.W., what can the problem be ?

Asked by: Soumya Ganguly
Thank you for your question. That is a very surprising result, as this is one of our most popular loading control antibodies against Beta Actin. Non-specific binding can result from different variables and there are a lot of different options for troubleshooting. We recommend blocking and diluting your primary and secondary antibodies in 5% nonfat milk + TBST. We also recommend using an appropriate secondary antibody from SCBT, and including a secondary control (incubated without primary antibody) to try and identify if the non-specific bands are due to your secondary antibody alone. I am providing a link below to our recommended protocol for Western blotting. If you still need help, please contact our Technical Service Department by phone (our Asian office toll-free number is 800-820-8626), by e-mail, asia@scbio.cn, or by live chat. https://www3.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2016-09-22
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Rated 5 out of 5 by from Neurochem Res (2013) 38:2535 2541<br /> Neurochem Res (2013) 38:2535 2541<br /> Localization of Mitochondrial Carnitine/Acylcarnitine<br /> Translocase in Sensory Neurons from Rat Dorsal Root Ganglia<br /> Annamaria Tonazzi Cristina Mantovani <br /> Matilde Colella Giorgio Terenghi <br /> Cesare Indiveri<br /> DOI 10.1007/s11064-013-1168-z
Date published: 2015-10-28
Rated 5 out of 5 by from It is a very good loading control It is a very good loading control.Our lab use this cat.no for several years.It can always get single and specific band. It can get perfect result in WB,IF,and IHC application.We highly recommend this cat.no as a loading control ,espeially in WB.
Date published: 2015-10-20
Rated 5 out of 5 by from I use I use -Actin Antibody (C4)-HRP at a dilution of 1:50,000/1:100,000 in TBS-Tween for 1 hr and is enough for an excellent western blot signal. Loading of 5 ug of total proteins is recommended for quantification.
Date published: 2015-09-11
Rated 5 out of 5 by from This b This b-actin antibody is good in wb application to test Human breast cancer cell sample. It's good quality we can get very good result. We used this antibody and publish the article on our college joural.
Date published: 2016-01-06
Rated 5 out of 5 by from Western blotting Western blotting, B-actin was used as an internal control for normalization. I am very satisfied with the product and with the service I received from SCBT.
Date published: 2015-09-24
Rated 4 out of 5 by from Used if for probing beta actin in rat brain Used if for probing beta actin in rat brain homogenates. Sometimes the actin band is light and sometimes you get a double band, but overall worked well
Date published: 2015-11-02
Rated 5 out of 5 by from We have been using this antibody for months We have been using this antibody for months and it has been working really well. Our Westerns are publishable and IP has been very efficient.
Date published: 2015-10-28
Rated 4 out of 5 by from Works for western blot analysis of beta Works for western blot analysis of beta-actin in lysates of RAW 264.7 cells and freshly isolated human monocytes.
Date published: 2015-11-05
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