beta-Actin Antibody (C4) recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA

β-Actin Antibody (C4): sc-47778

β-Actin Antibody (C4) is rated 4.8 out of 5 by 375.
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Datasheets
    • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against gizzard Actin of chicken origin
    • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-47778 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.
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What blocking buffer can be used for this antibody? Is 1X TBST with 5% BSA okay to detect bands?

Asked by: Edd22
Thank you for your question. We recommend 5% milk with TBS-T for most Westen blot applications, alternatively we also suggest using our UltraCruz Blocking reagent. You can find them both here: https://www.scbt.com/scbt/browse/Blocking-Reagents-for-Western-Blotting-Applications/_/N-1hrinjk
Answered by: Tech Service
Date published: 2019-04-09

Would this antibody recognize actin in filament (F-actin) or subunit (G-actin)?

Asked by: Anonymous
Thank you for your question. This antibody recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms; it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin.
Answered by: Technical Support
Date published: 2019-03-21

Would this antibody also recognize G-actin?

Asked by: XC268
Thank you for your question. If by G-actin you are referring to gamma-actin (γ- Actin) then this antibody may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform).
Answered by: Technical Support
Date published: 2019-03-03

β-actin (C4)抗体可以用于昆虫细胞吗,尤其蚊虫细胞

Asked by: 蛋白果果虫
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据,以解答您物种反应性的相关问题。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-02-27

We would like to get a 10ug free antibody sample for beta actin sc-47778. we are planning to use that antibody for westernblooting. the templete protein is RBC membrane We used to use Actin(C-11): sc-1615, and want to get antibody with similar working.

Asked by: Sakurako Neo
Thank you for your question. Please contact your local distributor for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-02-08

Is this antibody working in NON REDUCING conditions in Western Blot?

Asked by: Florence
Thank you for your question. β-Actin Antibody (C4): sc-47778 is recommended for detection of β-Actin by WB, IP, IF, IHC(P) and ELISA, which means it also recognizes the non-reduced epitope.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Sorry I wanted to ask if this antibody was working in NON REDUCING conditions in Western blot

Asked by: Florence
Thank you for your question. β-Actin Antibody (C4): sc-47778 is recommended for detection of β-Actin by WB, IP, IF, IHC(P) and ELISA, which means it also recognizes the non-reduced epitope.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Buongiorno, vorrei sapere se l'anticopo b actin con codice sc-47778 (C4) si può utilizzare sia su membrane con blocking in milk sia su membrane con blocking in bsa, oppure se funziona in modo più efficiente con uno dei due. Grazie

Asked by: Nadia21
Grazie per la sua domanda. Il modo più efficiente, testato e garantito di utilizzare i nostri anticorpi é con il TBS Blotto A sc-2333. Raccomandato per ridurre il background, e minimizzare i segnali non specifici comunemente associati ai metodi di rilevamento proteico utilizzando anticorpi primari e secondari, o proteine di legame mouse IgG. Contatti il nostro servizio tecnico scrivendoci a italia@scbt.com in caso volesse ricevere ulteriori informazioni.
Answered by: Technical Support Europe
Date published: 2018-12-21
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Rated 5 out of 5 by from Excellent antibody for Internal control This antibody was very specific and the signal produced by this antibody
Date published: 2019-05-20
Rated 5 out of 5 by from Great antibody! Initially, we got a free sample to test it with our lysates and then bought it. Very specific signal and no background at 1:1000 concentration even after 2 minutes exposure on a film (ECL based).
Date published: 2019-05-16
Rated 5 out of 5 by from Great customer service I didn’t have a single problem with my order with your company. Thermofisher was a horrible company and would never recommend them. Looking forward to my business with you.
Date published: 2019-05-14
Rated 5 out of 5 by from Using sc-47778 for normalization in western blot Very good sensitivity, clear bands even at about 1: 3000-5000 dilution, no background. Very Helpful.
Date published: 2019-04-23
Rated 5 out of 5 by from Excellent function It is very good. We use more than 100ug protein during WB.
Date published: 2019-04-22
Rated 5 out of 5 by from Excellent control antibody for Western Blot Antibody produces clean and strong bands for human and mouse samples regardless of other conditions.
Date published: 2019-04-19
Rated 5 out of 5 by from Very good antibody! Highly specific. It works good for western blotting.
Date published: 2019-04-19
Rated 5 out of 5 by from lab Tec This antibody was very specific and the signal produced by this antibody was very strong for our human lung and dermal endothelial cells.
Date published: 2019-04-12
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