beta-Actin Antibody (C4)
  • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
  • raised against gizzard Actin of chicken origin
  • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
  • available conjugated to agarose (sc-47778 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-47778 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-47778 PE), fluorescein (sc-47778 FITC), Alexa Fluor® 488 (sc-47778 AF488) or Alexa Fluor® 647 (sc-47778 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • also available conjugated to biotin (sc-47778 B, 200 µg/ml) for WB, IHC(P) and ELISA
  • TransCruz reagent for ChIP application (sc-47778 X, 200 µg/0.1 ml)
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) (sc-516102), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
  • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.

β-Actin Antibody (C4): sc-47778

β-Actin Antibody (C4) is rated 4.8 out of 5 by 289.
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Datasheets
    • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against gizzard Actin of chicken origin
    • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • available conjugated to agarose (sc-47778 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-47778 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-47778 PE), fluorescein (sc-47778 FITC), Alexa Fluor® 488 (sc-47778 AF488) or Alexa Fluor® 647 (sc-47778 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • also available conjugated to biotin (sc-47778 B, 200 µg/ml) for WB, IHC(P) and ELISA
    • TransCruz reagent for ChIP application (sc-47778 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) (sc-516102), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.
Submit a review for this product and receive 15 CruzCredits

Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
β-Actin Antibody (C4) sc-47778 200 µg/ml $279.00
β-Actin Antibody (C4) AC sc-47778 AC 500 µg/ml, 25% ag $366.00
β-Actin Antibody (C4) Alexa Fluor® 488 sc-47778 AF488 200 µg/ml $314.00
β-Actin Antibody (C4) Alexa Fluor® 647 sc-47778 AF647 200 µg/ml $314.00
β-Actin Antibody (C4) B sc-47778 B 200 µg/ml $282.00
β-Actin Antibody (C4) FITC sc-47778 FITC 200 µg/ml $292.00
β-Actin Antibody (C4) HRP sc-47778 HRP 200 µg/ml $279.00
β-Actin Antibody (C4) PE sc-47778 PE 200 µg/ml $303.00
β-Actin Antibody (C4) X sc-47778 X 200 µg/0.1 ml $279.00

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beta-Actin Antibody (C4) Product Citations

See how others have used beta-Actin Antibody (C4). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 1834 total

PMID: # 28035402
Chiba, T. et al. 2017. Transforming growth factor-β1 suppresses bone morphogenetic protein-2-induced mesenchymal-epithelial transition in HSC-4 human oral squamous cell carcinoma cells via Smad1/5/9 pathway suppression. Oncol. Rep.. 37: 713-720.

PMID: # 28400700
Du, M. et al. 2017. Serum retinol-binding protein-induced endothelial inflammation is mediated through the activation of toll-like receptor 4. Mol. Vis.. 23: 185-197.

PMID: # 27927748
de Polo, A. et al. 2017. AXL receptor signalling suppresses p53 in melanoma through stabilization of the MDMX-MDM2 complex. J Mol Cell Biol. 9: 154-165.

PMID: # 28365474
Ma, XD. et al. 2017. TDAG8 activation attenuates cerebral ischaemia-reperfusion injury via Akt signalling in rats. Exp. Neurol.. 293: 115-123.

PMID: # 28279309
Buzby, JS. et al. 2017. Allele-specific wild-type TP53 expression in the unaffected carrier parent of children with Li-Fraumeni syndrome. Cancer Genet. 211: 9-17.

PMID: # 28336431
Xue, Z. et al. 2017. Calcium-sensing receptor antagonist NPS2390 attenuates neuronal apoptosis though intrinsic pathway following traumatic brain injury in rats. Biochem. Biophys. Res. Commun.. 486: 589-594.

PMID: # 28438602
Menon, RT. et al. 2017. Hyperoxia exposure disrupts adrenomedullin signaling in newborn mice: Implications for lung development in premature infants. Biochem. Biophys. Res. Commun.. 487: 666-671.

PMID: # 28521482
Hu, B. et al. 2017. MicroRNA-204 suppressed proliferation and motility capacity of human hepatocellular carcinoma via directly targeting zinc finger E-box binding homeobox 2. Oncol Lett. 13: 3823-3830.

PMID: # 28196545
Wu, J. et al. 2017. Roles of programmed death protein 1/programmed death-ligand 1 in secondary brain injury after intracerebral hemorrhage in rats: selective modulation of microglia polarization to anti-inflammatory phenotype. J Neuroinflammation. 14: 36.

PMID: # 28356983
Guo, Q. et al. 2017. TCN, an AKT inhibitor, exhibits potent antitumor activity and enhances radiosensitivity in hypoxic esophageal squamous cell carcinoma in vitro and in vivo. Oncol Lett. 13: 949-954.

Citations 1 to 10 of 1834 total

Whether beta-Actin (c4) sc-47778 may be use directly for western blotting without secondary antibody?

Asked by: singh Santosh
Thank you for your question. If you wish to use this primary antibody directly for Western blotting without a secondary antibody, we would recommend purchasing the HRP conjugated version of the antibody, β-Actin Antibody (C4) HRP: sc-47778 HRP.
Answered by: Technical Support
Date published: 2017-08-01

The concentration of b-actin antibody is 200ug/ml, please let me how many volume you will supply for each lot.

Asked by: Shimada
Thank you for your question! You receive 200µg of antibody in 1 ml PBS, gelatin and NaA. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-03-22

what is the secondary antibody dilution?

Asked by: lee66
Thank you for your inquiry. The dilution to use for the secondary depends on which secondary reagent you are working with, and for what application you are using it for. Generally, we say to do a titration to find the optimal conditions, but since this is a very strong primary antibody, I would recommend starting the anti-mouse secondary at 1:2000, and optimizing further from that point, depending on results. Here is a link to our recommended protocols: https://www.scbt.com/scbt/resources/protocols If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-02-23

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: cjMara
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2016-12-13

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: cjMara
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This mouse monoclonal antibody, β-Actin Antibody (C4): sc-47778, is currently available conjugated to HRP (sc-47778 HRP), phycoerythrin (sc-47778 PE), fluorescein (sc-47778 FITC), Alexa Fluor® 488 (sc-47778 AF488), Alexa Fluor® 647 (sc-47778 AF647), and biotin (sc-47778 B).
Answered by: Technical Support
Date published: 2016-12-13

I am using sc-47778 , but i am getting many bands at different M.W., what can the problem be ?

Asked by: Soumya Ganguly
Thank you for your question. That is a very surprising result, as this is one of our most popular loading control antibodies against Beta Actin. Non-specific binding can result from different variables and there are a lot of different options for troubleshooting. We recommend blocking and diluting your primary and secondary antibodies in 5% nonfat milk + TBST. We also recommend using an appropriate secondary antibody from SCBT, and including a secondary control (incubated without primary antibody) to try and identify if the non-specific bands are due to your secondary antibody alone. I am providing a link below to our recommended protocol for Western blotting. If you still need help, please contact our Technical Service Department by phone (our Asian office toll-free number is 800-820-8626), by e-mail, asia@scbio.cn, or by live chat. https://www3.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2016-09-22
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Rated 5 out of 5 by from good quality This antibody was diluted 1:500 with 5% milk in PBST. I incubated for 75min at RT, the Secondary goat anti-mouse IgG-HRP antibody from BOSTER ( 1:8000). The band is clean and specific.
Date published: 2017-09-20
Rated 5 out of 5 by from Specific no background I got a sample to test on WB. Diluted in PBST+0.5% milk for 1:250, overnight incubation at 4C.
Date published: 2017-09-19
Rated 5 out of 5 by from Works great on WB I used it at 1:2500 O/N for Westerns and It worked great. Single sharp band. I loaded 20 ug of extract. I think it might work well at a higher dilution too.
Date published: 2017-09-17
Rated 5 out of 5 by from very good This actin antibody we have used it in our WB analysis for Many times.It always gave us strong bands.When we tried different samples,No problem ,1:500 to 1:2000 all tried.
Date published: 2017-09-16
Rated 4 out of 5 by from Good quality The antibody gives good result for western blotting assays (dilution 1/600). Good signal strength, it could have also worked well in a lower concentration. We obtained only one band with whole cell lysates (HepG2) and three bands with tissue samples (mice liver). However, it was clear which band was the specific one.
Date published: 2017-09-12
Rated 4 out of 5 by from good quality This antibody always in good quality.It shows very clear bands.
Date published: 2017-09-12
Rated 5 out of 5 by from Always good quality This actin antibody was diluted 1:1000 with 5% milk in TBST,We incubated for 1 hour at RT,the Secondary goat anti-mouse IgG-HRP antibody from CWBIO ( 1:5000).Very clean background ,specific bands presented at 43kda.
Date published: 2017-09-09
Rated 5 out of 5 by from very good performance I peform western blot recently,and this product has a very clear band which is beyond my expectation.I perform at 1:1000, and I believe it could be clear in more lower ratio.
Date published: 2017-09-07
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