beta-Actin Antibody (C4)recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA

β-Actin Antibody (C4): sc-47778

β-Actin Antibody (C4) is rated 4.8 out of 5 by 382.
  • y_2019, m_8, d_22, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_2, tr_380
  • loc_en_US, sid_47778, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 11ms
  • REVIEWS, PRODUCT
Datasheets
    • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against gizzard Actin of chicken origin
    • beta-Actin Antibody (C4) is recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • beta-Actin Antibody (C4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-47778 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.
Submit a review for this product and receive 15 CruzCredits

Can anyone recommend an santa cruz anti-actin antibody for detection with plant material?

Asked by: MichaelN
Thank you for your question. This antibody clone has previously been published in arabidopsis samples, so it would be a good choice.
Answered by: Tech Service
Date published: 2019-08-14

What blocking buffer can be used for this antibody? Is 1X TBST with 5% BSA okay to detect bands?

Asked by: Edd22
Thank you for your question. We recommend 5% milk with TBS-T for most Westen blot applications, alternatively we also suggest using our UltraCruz Blocking reagent. You can find them both here: https://www.scbt.com/scbt/browse/Blocking-Reagents-for-Western-Blotting-Applications/_/N-1hrinjk
Answered by: Tech Service
Date published: 2019-04-09

Would this antibody recognize actin in filament (F-actin) or subunit (G-actin)?

Asked by: Anonymous
Thank you for your question. This antibody recognizes all six isoforms of vertebrate actin, globular (G) and filamentous (F) forms; it is a pan-actin antibody that binds to an epitope in a highly conserved region of actin.
Answered by: Technical Support
Date published: 2019-03-21

Would this antibody also recognize G-actin?

Asked by: XC268
Thank you for your question. If by G-actin you are referring to gamma-actin (γ- Actin) then this antibody may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform).
Answered by: Technical Support
Date published: 2019-03-03

β-actin (C4)抗体可以用于昆虫细胞吗,尤其蚊虫细胞

Asked by: 蛋白果果虫
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website. 感谢您的提问。请将您的目的蛋白序列发送到我们的技术支持部门。我们将对比您的序列以及我们的数据,以解答您物种反应性的相关问题。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-02-27

We would like to get a 10ug free antibody sample for beta actin sc-47778. we are planning to use that antibody for westernblooting. the templete protein is RBC membrane We used to use Actin(C-11): sc-1615, and want to get antibody with similar working.

Asked by: Sakurako Neo
Thank you for your question. Please contact your local distributor for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-02-08

Is this antibody working in NON REDUCING conditions in Western Blot?

Asked by: Florence
Thank you for your question. β-Actin Antibody (C4): sc-47778 is recommended for detection of β-Actin by WB, IP, IF, IHC(P) and ELISA, which means it also recognizes the non-reduced epitope.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Sorry I wanted to ask if this antibody was working in NON REDUCING conditions in Western blot

Asked by: Florence
Thank you for your question. β-Actin Antibody (C4): sc-47778 is recommended for detection of β-Actin by WB, IP, IF, IHC(P) and ELISA, which means it also recognizes the non-reduced epitope.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06
  • y_2019, m_8, d_22, h_20CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_19
  • loc_en_US, sid_47778, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 38ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from good control our group always use it as the control antibody~~~
Date published: 2019-08-15
Rated 5 out of 5 by from Western blot of β-actin Good Western blot data of β-actin expression in rat hippocampal brain tissue. Specific band at 1:1000 dilution.
Date published: 2019-08-13
Rated 5 out of 5 by from Western blot of β-Actin Good Western blot data of β-actin expression in rat hippocampal brain tissue. Specific band at 1:1000 dilution.
Date published: 2019-08-13
Rated 5 out of 5 by from greatest antibody Our group have been buying the antibody and we are all glad to buy it.
Date published: 2019-08-13
Rated 5 out of 5 by from Great antibody! work very well. always used as a loading control in western blot
Date published: 2019-08-01
Rated 5 out of 5 by from work well work well~~~ always used as a loading control in WB
Date published: 2019-08-01
Rated 5 out of 5 by from Good signal in WB (1:1000 dilution) Good signal in WB (1:1000 dilution) in mouse brain tissue samples.
Date published: 2019-06-28
Rated 5 out of 5 by from Excellent antibody for Internal control This antibody was very specific and the signal produced by this antibody
Date published: 2019-05-20
  • y_2019, m_8, d_22, h_20
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.13
  • cp_1, bvpage1
  • co_hasreviews, tv_2, tr_380
  • loc_en_US, sid_47778, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 32ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.