beta-Actin Antibody (C4) recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA

β-Actin Antibody (C4): sc-47778

β-Actin Antibody (C4) is rated 4.8 out of 5 by 362.
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Datasheets
    • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against gizzard Actin of chicken origin
    • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-47778 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.
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We would like to get a 10ug free antibody sample for beta actin sc-47778. we are planning to use that antibody for westernblooting. the templete protein is RBC membrane We used to use Actin(C-11): sc-1615, and want to get antibody with similar working.

Asked by: Sakurako Neo
Thank you for your question. Please contact your local distributor for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2019-02-08

Is this antibody working in NON REDUCING conditions in Western Blot?

Asked by: Florence
Thank you for your question. β-Actin Antibody (C4): sc-47778 is recommended for detection of β-Actin by WB, IP, IF, IHC(P) and ELISA, which means it also recognizes the non-reduced epitope.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Sorry I wanted to ask if this antibody was working in NON REDUCING conditions in Western blot

Asked by: Florence
Thank you for your question. β-Actin Antibody (C4): sc-47778 is recommended for detection of β-Actin by WB, IP, IF, IHC(P) and ELISA, which means it also recognizes the non-reduced epitope.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Buongiorno, vorrei sapere se l'anticopo b actin con codice sc-47778 (C4) si può utilizzare sia su membrane con blocking in milk sia su membrane con blocking in bsa, oppure se funziona in modo più efficiente con uno dei due. Grazie

Asked by: Nadia21
Grazie per la sua domanda. Il modo più efficiente, testato e garantito di utilizzare i nostri anticorpi é con il TBS Blotto A sc-2333. Raccomandato per ridurre il background, e minimizzare i segnali non specifici comunemente associati ai metodi di rilevamento proteico utilizzando anticorpi primari e secondari, o proteine di legame mouse IgG. Contatti il nostro servizio tecnico scrivendoci a italia@scbt.com in caso volesse ricevere ulteriori informazioni.
Answered by: Technical Support Europe
Date published: 2018-12-21

If i want to order, how many volume you have for 1tube of β-Actin Antibody (C4) (sc-47778) ?

Asked by: Ferda
Thank you for your question. -Actin (C4): sc-47778 is provided in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-05

How do I request a free 10 ug sample of the beta-actin (C4) antibody (cat. # sc-47778)?

Asked by: Wil Ratzan
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I have a vial of (β-Actin Antibody (C4): sc-47778) the lot number for the vial is (J1113). I couldn't find any expiration date for the product. could you tell me what is the expiration date and also I need to mention, we kept it in 4c. Thanks

Asked by: Mehran
Thank you for your question. We do not provide expiration dates for our antibody or chemical products. Instead, we provide all of our products with a 1-year warranty from the date of shipment. This product was produced at the end of 2013, but as long as it has been stored at 4˚C, it should be fine to still use. If you have any further questions or concerns, please feel free to contact our Technical Service department.
Answered by: Tech Service
Date published: 2017-11-28

Can this antibody hide Trichomonas vaginalis beta-actin??

Asked by: abcdefghijklmnopqrstuvwxy
Thank you for your question. The antibody has not been tested for this use, so we do not know if it can be used for blocking purposes. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-10-03
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Rated 5 out of 5 by from 良いと思います。 サンプルでいただきました、β-actinに対する抗体ですが、蛍光免疫染色に使ってみました。100倍希釈。繊維様のシグナルを認めます。
Date published: 2019-02-15
Rated 5 out of 5 by from Beta actin in renal tissue using western blot We used this antibody to standardized protein loaded. We could visualized a very good band closely to 42 kDa.
Date published: 2019-02-08
Rated 5 out of 5 by from antibody review anti-β-actin, clone C4 - nicely working antibody for WB application, i use it for a loading control
Date published: 2019-01-23
Rated 5 out of 5 by from total protein level excellent antibody for total protein level, very sensitive - saving money
Date published: 2019-01-21
Rated 5 out of 5 by from Excellent antibody for WB! I used this antibody month ago, it works very good.
Date published: 2019-01-16
Rated 5 out of 5 by from very good antibody. This antibody give very clean background and give strong Actin band.
Date published: 2019-01-03
Rated 5 out of 5 by from Worked perfectly for Jurkat, PC3 and MCF-7 cells Worked perfectly for Jurkat, PC3 and MCF-7 cells. The signal intensity was good and antibody was stable and working even when my colleague stored it at room temperature for few weeks.
Date published: 2019-01-02
Rated 5 out of 5 by from great antibody great antibody, detected a nice band on western blot in a number of cells
Date published: 2018-12-22
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