beta-Actin Antibody (C4) recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA

β-Actin Antibody (C4): sc-47778

β-Actin Antibody (C4) is rated 4.8 out of 5 by 352.
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Datasheets
    • β-Actin Antibody (C4) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against gizzard Actin of chicken origin
    • recommended for detection of β-Actin of mouse, rat, human, avian, bovine, canine, porcine, rabbit, Dictyostelium discoideum and Physarum polycephalum origin by WB, IP, IF, IHC(P) and ELISA; may cross-react with all six known isoforms of Actin in higher vertebrates (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform)
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to biotin for WB, IHC(P) and ELISA
    • TransCruz reagent for Gel Supershift and ChIP applications (sc-47778 X, 200 µg/0.1 ml)
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of β-Actin (C4): sc-47778.
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If i want to order, how many volume you have for 1tube of β-Actin Antibody (C4) (sc-47778) ?

Asked by: Ferda
Thank you for your question. -Actin (C4): sc-47778 is provided in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-05

How do I request a free 10 ug sample of the beta-actin (C4) antibody (cat. # sc-47778)?

Asked by: Wil Ratzan
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

I have a vial of (β-Actin Antibody (C4): sc-47778) the lot number for the vial is (J1113). I couldn't find any expiration date for the product. could you tell me what is the expiration date and also I need to mention, we kept it in 4c. Thanks

Asked by: Mehran
Thank you for your question. We do not provide expiration dates for our antibody or chemical products. Instead, we provide all of our products with a 1-year warranty from the date of shipment. This product was produced at the end of 2013, but as long as it has been stored at 4˚C, it should be fine to still use. If you have any further questions or concerns, please feel free to contact our Technical Service department.
Answered by: Tech Service
Date published: 2017-11-28

Can this antibody hide Trichomonas vaginalis beta-actin??

Asked by: abcdefghijklmnopqrstuvwxy
Thank you for your question. The antibody has not been tested for this use, so we do not know if it can be used for blocking purposes. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-10-03

Whether beta-Actin (c4) sc-47778 may be use directly for western blotting without secondary antibody?

Asked by: singh Santosh
Thank you for your question. If you wish to use this primary antibody directly for Western blotting without a secondary antibody, we would recommend purchasing the HRP conjugated version of the antibody, β-Actin Antibody (C4) HRP: sc-47778 HRP.
Answered by: Technical Support
Date published: 2018-01-12

The concentration of b-actin antibody is 200ug/ml, please let me how many volume you will supply for each lot.

Asked by: Shimada
Thank you for your question! You receive 200µg of antibody in 1 ml PBS, gelatin and NaA. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2018-03-05

what is the secondary antibody dilution?

Asked by: lee66
Thank you for your inquiry. The dilution to use for the secondary depends on which secondary reagent you are working with, and for what application you are using it for. Generally, we say to do a titration to find the optimal conditions, but since this is a very strong primary antibody, I would recommend starting the anti-mouse secondary at 1:2000, and optimizing further from that point, depending on results. Here is a link to our recommended protocols: https://www.scbt.com/scbt/resources/protocols If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: europe@scbt.com or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-02-23

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: cjMara
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support
Date published: 2016-12-13
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Rated 4 out of 5 by from OK for human, but less for murine b-actin The antibody is excellent for western blotting at 1:1000 with cell lysates of human origin and it also works with murine tissue samples, but has much lower affinity for mouse beta-actin (around 10 times lower). It works better with 4% BSA than 4% milk in TTBS.
Date published: 2018-12-04
Rated 4 out of 5 by from Wonderful Westerns I purchased this antibody a few weeks before and quite happy with its performance.
Date published: 2018-11-26
Rated 5 out of 5 by from Beta actin antibody for WB great antibody for western blot and Immuno-fluorescence staining
Date published: 2018-11-14
Rated 5 out of 5 by from Great! It works good for western blotting. I can get a strong one band.
Date published: 2018-10-01
Rated 5 out of 5 by from great antibody antibody works great in 1:1000 dilution in different tissues and cell lines
Date published: 2018-09-05
Rated 4 out of 5 by from Anti-beta-actin The antibody works great for western blotting Dictyostelium actin.
Date published: 2018-07-25
Rated 5 out of 5 by from Good antibody works reliable. Due to the direct labeling, easy to handle and time saving
Date published: 2018-07-04
Rated 5 out of 5 by from qualité antib-actib(C4)-HRP nous n'utilisons plus que celui -là reproductible et fiable, gain de temps
Date published: 2018-06-26
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