Benzonase® Nuclease Lyophilized Powder is a solvent-free version of Benzonase® Nuclease (sc-202391). For liquid format stabilized in glycerol, please consider purchase of (sc-202391), Benzonase® Nuclease.
The Benzonase® enzyme is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.
The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.
Benzonase is a registered trademark of Merck KGaA.
Reconstitution: It is recommended to reconstitute the lyophilized Benzonase® Nuclease in 100 μl (per 25KU) sterile 50% glycerol to a final concentration of 250U/μl. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing by hand. Avoid vigorous shaking or vortexing.
Removal of Benzonase® Nuclease: Benzonase® Nuclease contain no "Tag" and used in downstream processing can be removed by various purification methods according to the purification strategy for the target protein.
Reaction buffer: 50 mM Tris-HCl, 1 mM MgCl2, pH 8.0. For dilute solutions, addition of a carrier protein such as 0.1 mg/ml HSA or BSA is recommended to avoid any enzyme loss from surface adsorption.
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of Benzonase® Nuclease is 1.3106 ~ 1.5106 unit/mg protein.
Lyophilized Benzonase® Nuclease is shipped at Ambient temperature. Upon receipt, store lyophilized product at -20°C, stable for 1 year. Following reconstitution, store at -20°C to -70°C. Avoid repeated freeze-thaw cycles.
Recombinant protein expressed in Escherichia coli
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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This chemical, sc-391121, is soluble in 50 mM Tris-HCl, and 1 mM MgCl2, pH 8.0. For dilute solutions, addition of a carrier protein such as 0.1 mg/ml HSA or BSA is recommended to avoid any enzyme loss from surface adsorption.
Answered by: Chemical Support 1
Date published: 2017-03-03
How long might the lyophilized form be stable at -20ºC?
Asked by: chemicalsmg
Thank you for your question. sc-391121is stable for one year when stored at -20.
Rated 5 out of
The product is working great for multipleThe product is working great for multiple purification assays.
Date published: 2015-10-05
Rated 5 out of
Various publications cite the use of BenzonaseVarious publications cite the use of Benzonase (Benzonase nuclease lyophilized powder; Golden Nuclease) to completely digest nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts. -SCBT Publication Review
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