Benzonase® Nuclease is a solubilized version of Benzonase® Nuclease Lyophilized Powder (sc-391121). The Benzonase® enzyme is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.
Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.
The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.
Benzonase is a registered trademark of Merck KGaA.
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See how others have used Benzonase Nuclease. Click on the entry to view the PubMed entry .
PMID: # 27911858 Charles, E. et al. 2017. Oncotarget. 8: 3181-3196.
PMID: # 26215075 Eek, P. et al. 2015. Biochimica et biophysica acta. 1851: 1377-82.
PMID: # 25659430 Hellmuth, S. et al. 2015. The Journal of biological chemistry. 290: 8002-10.
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