Benzonase Nuclease Biologically active protein: an endonuclease ideal for applications where complete digestion of nucleic acids is desirable

Benzonase® Nuclease (CAS 9025-65-4)

Benzonase Nuclease | CAS 9025-65-4 is rated 5.0 out of 5 by 1.
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Synonym: BenzNuclease
Application: An endonuclease ideal for applications where complete digestion of nucleic acids is desirable
CAS Number: 9025-65-4
Purity: ≥90%
* Refer to Certificate of Analysis for lot specific data (including water content).
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Benzonase® Nuclease is a solubilized version of Benzonase® Nuclease Lyophilized Powder (sc-391121). The Benzonase® enzyme is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

Benzonase® Nuclease is a genetically engineered endonuclease from Serratia marcescens. Benzonase® Nuclease, also called Golden nuclease, is a highly useful endonuclease for removal of nucleic acids from recombinant proteins and protein fragments, and is also used for the reduction of viscosity in protein extracts.

The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2–5 bases in length. Although the nuclease is capable of cleavage at nearly all positions along a nucleic acid chain, sequence-dependent preferences have been demonstrated. The enzyme prefers GC-rich regions in dsDNA while avoiding d(A)/d(T)-tracts. Benzonase is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable. Digests native or heat-denatured DNA and RNA. This can also be used for the removal of nucleic acid from protein samples.

Unit definition:
One unit of Benzonase Nuclease is defined as the amount of enzyme that causes a ΔA260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μg of DNA. Note that 1 KU = 1000 units.

Benzonase is a registered trademark of Merck KGaA.


References

1. Miller, M.D., et al. 1994. Nat. Struct. Biol. 1: 461-468. PMID: 7664065
2. Friedhoff, P., et al. 1994. Nucleic Acids Res. 22: 3280-3287. PMID: 8078761
3. Ball, T.K., et al. 1992. Nucleic Acids Res. 20: 4971-4974. PMID: 1329033
4. Ball, T.K., et al. 1987. Gene. 57: 183-192. PMID: 3319779
5. Janning, P., et al. 1994. Rapid Commun. Mass Spectrom. 8: 1035-1040. PMID: 7696699
6. Molloy, M.P., et al. 1998. Electrophoresis. 19: 837-844. PMID: 9629924
7. Herbert, B.R., et al. 1998. Electrophoresis. 19: 845-851. PMID: 9629925

Usage :
The activity of any unknown nuclease can be determined from a single measurement by means of the standard curve. The specific activity of Benzonase® Nuclease is 1.3x106 ~ 1.5x106 unit/mg protein.
Formulation :
≥250 units/µL supplied in solution containing 20 mM Tris HCl (pH 8.0), 2 mM MgCl2, 20 mM NaCl and 50% glycerol.
Physical State :
Liquid
Derived From :
Recombinant protein expressed in Escherichia coli
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
1
MDL Number :
MFCD00131010
EC Number :
231-791-2

Download SDS (MSDS)

Certificate of Analysis

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Benzonase Nuclease  Product Citations

See how others have used Benzonase Nuclease. Click on the entry to view the PubMed entry .

Citations 1 to 3 of 3 total

PMID: # 27911858  Charles, E. et al. 2017. Oncotarget. 8: 3181-3196.

PMID: # 26215075  Eek, P. et al. 2015. Biochimica et biophysica acta. 1851: 1377-82.

PMID: # 25659430  Hellmuth, S. et al. 2015. The Journal of biological chemistry. 290: 8002-10.

Citations 1 to 3 of 3 total

Can Benzonase digest DNA bound/protected by proteins?

Asked by: seo12
Thank you for your question. Yes, this BenzNuclease product can digest DNA bound/protected by proteins. Its high intrinsic activity and broad substrate tolerance make the endonuclease an ideal tool in a variety of biotechnological applications: removal of nucleic acid from protein samples (Elimination of nucleic acids from recombinant proteins; Purification of protein fragments from inclusion bodies; Sample preparation in western blotting or two-dimensional gel electrophoresis) ; Viscosity reduction in protein extracts.
Answered by: Technical Support
Date published: 2019-04-24

Does the benzonase endonuclease have any proteolytic activity?

Asked by: Chick1
Thank you for your question. The product Benzonase® Nuclease is free of detectable proteolytic activity. Please contact our Technical Service if you need further information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

Does Benzonase Nuclease 500KU is intended to be used for therapeutic use? Do you have USP,NF,EU pharmacopoeia label claim?

Asked by: Nayak
This chemical is for research use only. It is not intended for diagnostic or therapeutic use.
Answered by: Technical Support
Date published: 2018-12-11

Does this products have any tags used for purification?

Asked by: hawkeye11
Thank you for your question. This chemical, Benzonase® Nuclease (CAS 9025-65-4), has no tags used for purification.
Answered by: Chemical Support 1
Date published: 2017-02-23

What components are in this product?

Asked by: hawkeye11
Thank you for your question. This chemical, Benzonase® Nuclease (CAS 9025-65-4) is supplied in solution containing Tris HCl (pH 8.0), MgCl2, NaCl and glycerol.
Answered by: Chemical Support 1
Date published: 2017-02-23

What concentration of Mg (2+) does the enzyme need to be a nuclease?

Asked by: hawkeye11
Thank you for your question. For this chemical, Benzonase® Nuclease CAS# 9025-65-4, 1-2 mM of Mg2+ is required for enzyme activity.
Answered by: Chemical Support 1
Date published: 2017-02-23
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Rated 5 out of 5 by from Various publications cite the use of Benzonase Various publications cite the use of Benzonase Nuclease in their research when complete digestion of nucleotides is desirable. -SCBT Publication Review
Date published: 2015-04-16
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