BCIP-NBT Solution BCIP-NBT Solution acts as the substrate for AP-based detection systems, giving a dark-blue oxidation product.

BCIP-NBT Solution (CAS 67-56-1)

BCIP-NBT Solution is rated 5.0 out of 5 by 1.
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| See product citations (13)
Application: A substrate for alkaline phosphatase (AP) based detection systems including western blotting
CAS Number: 67-56-1
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
BCIP-NBT Solution sc-358798 100 ml $55.00

BCIP-NBT Solution is a 5-bromo, 4-chloro, 3-indolylphosphate (BCIP)/Nitro-Blue Tetrazolium (NBT) substrate, used for the localization of alkaline phosphatase (AP) labeled probes on western, northern, southern and dot blots. BCIP acts as the substrate for AP and reacts further after the dephosphorylation to give a dark-blue indigo-dye as an oxidation product; NBT serves herein as the oxidant and also gives a dark-blue dye, intensifying the color and making the detection more sensitive. Recommended for use with Crystal Mounting Medium (sc-24943).


References

1. Horwitz, J.P., et al. 1966. J. Med. Chem. 9: 447. PMID: 5960940
2. Wolf, P.L., et al. 1968. Enzymologia. 35: 154-156. PMID: 4176788
3. Wolf, P.L., et al. 1973. Clin. Chem. 19: 1248-1249. PMID: 4758599
4. Bergmeyer, H. 1986. Methods of Enzymatic Analysis. 3rd edition. Michal, G., et al. ed. Academic Press. New York.

Usage :
• Product should be brought to room temperature (~25 °C) before use.
• Use enough substrate solution to completely cover the membrane surface.
• Upon incubation, a dark blue-purple reaction product will develop on membrane sites where alkaline phosphatase is present. Monitor reaction continuously and record results before background staining prevails.
• Stop the reaction by rinsing briefly with water.
• Dilution of the substrate is not recommended. To reduce the intensity of a reaction, it is recommended the antibodies or conjugates be diluted.
Formulation :
0.42 g/L NBT, and 0.21 g/L BCIP in 0.1M AMP Buffer Solution
Physical State :
Liquid
Storage :
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.

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BCIP-NBT SolutionProduct Citations

See how others have used BCIP-NBT Solution. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 13 total

PMID: # 26513380
Eskicioğlu, F. et al. 2015. The association of HLA-G and immune markers in recurrent miscarriages. J. Matern. Fetal. Neonatal. Med.. 1-5.

PMID: # 25759564
Ahmadipour, F. et al. 2015. Koenimbin, a natural dietary compound of Murraya koenigii (L) Spreng: inhibition of MCF7 breast cancer cells and targeting of derived MCF7 breast cancer stem cells (CD44(+)/CD24(-/low)): an in vitro study. Drug Des Devel Ther. 9: 1193-208.

PMID: # 26108872
Zahedifard, M. et al. 2015. Synthesis, characterization and apoptotic activity of quinazolinone Schiff base derivatives toward MCF-7 cells via intrinsic and extrinsic apoptosis pathways. Sci Rep. 5: 11544.

PMID: # 25271645
Anwar, S. et al. 2014. Interaction between Nbp35 and Cfd1 proteins of cytosolic Fe-S cluster assembly reveals a stable complex formation in Entamoeba histolytica. PLoS ONE. 9: e108971.

PMID: # 24901644
Equbal, A. et al. 2014. Stage-dependent expression and up-regulation of trypanothione synthetase in amphotericin B resistant Leishmania donovani. PLoS ONE. 9: e97600.

PMID: # 25395836
Ibrahim, MY. et al. 2014. Involvement of NF-κB and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, α-mangostin, from Cratoxylum arborescens. Drug design, development and therapy. 8: 2193-211.

PMID: # 24944509
Paydar, M. et al. 2014. Evaluation of cytotoxic and chemotherapeutic properties of boldine in breast cancer using in vitro and in vivo models. Drug Des Devel Ther. 8: 719-33.

PMID: # 24701154
Kadir, FA. et al. 2014. PASS-predicted hepatoprotective activity of Caesalpinia sappan in thioacetamide-induced liver fibrosis in rats. ScientificWorldJournal. 2014: 301879.

PMID: # 24058648
Golbabapour, S. et al. 2013. Gastroprotection studies of Schiff base zinc (II) derivative complex against acute superficial hemorrhagic mucosal lesions in rats. PLoS ONE. 8: e75036.

PMID: # 22392116
Liu, H. et al. 2012. Aquaporin 9 in rat brain after severe traumatic brain injury. Arq Neuropsiquiatr. 70: 214-20.

Citations 1 to 10 of 13 total
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Rated 5 out of 5 by from Various publications cite the use of BCIP Various publications cite the use of BCIP-NBT Solution for the localization of alkaline phosphatase (AP) labeled probes on western, northern, southern and dot blots. -SCBT Publication Review
Date published: 2015-02-02
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