Basic Cytokeratin Antibody (AE3) recommended for detection of basic (type II) cytokeratins of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)

Basic Cytokeratin Antibody (AE3): sc-57004

Basic Cytokeratin Antibody (AE3) is rated 4.8 out of 5 by 6.
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Datasheets
    • Basic Cytokeratin Antibody (AE3) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against epidermal keratins of human origin
    • recommended for detection of basic (type II) cytokeratins of mouse, rat, human and bovine origin by WB, IP, IF and IHC(P)
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact us to receive a FREE 10 µg sample of Basic Cytokeratin (AE3): sc-57004.
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Can Basic Cytokeratin (AE3): sc-57004 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: AbPolly
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Basic Cytokeratin (AE3): sc-57004 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from works for IHC 用于动物组织的免疫组化,特异性表达还可以,第一次没做太多张片子,准备再多试几次,看看其他组的结果如何。
Date published: 2017-08-01
Rated 5 out of 5 by from Very popular choice! This monoclonal Basic Cytokeratin Antibody (AE3) is a very popular choice for IF and IHC applications, and has been featured in 9 publications that we are aware of to date.
Date published: 2017-02-08
Rated 5 out of 5 by from Wonderful for paraffin Wonderful for paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Good specificity in HeLa whole cell lysate Good specificity in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-04
Rated 5 out of 5 by from Great WB antibody Great WB antibody! Published in human head and neck squamous cell carcinoma -SCBT Publication Review
Date published: 2015-01-23
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal strength in MCF7. -SCBT QC
Date published: 2014-10-06
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