BAPTA-APM [5-(3-Aminopropyl)-5'-methyl-bis-(2-aminophenoxy-methylene)-N,N,N',N'-tetraacetate, tetrapotassium salt] is a fluorescent chelating indicator used to study cytosolic free calcium. Fluorescent calcium indicators selectively bind to calcium ions and show a spectral response upon binding Ca2+; thus, fluorescence intensity or wavelength are altered. This shift of fluorescence wavelength and change of intensity upon calcium chelation can be monitored using fluorescence microscopy. This high quality fluorescent Ca2+ chelator is useful for many intracellular applications, especially in single cells, adherent cell layers, or bulk tissues.
Additional BAPTA chelators available:
BAPTA, Free Acid (sc-201508)
BAPTA tetrapotassium salt (sc-202076)
5,5i difluoro Bapta(AM) (sc-217247)
5,5i difluoro Bapta(K+ Salt) (sc-217248)
Bapta-FF (AM) (sc-217696)
BAPTA, Tetrasodium Salt (sc-278716)
1. Cannon, C., et al. 1985. Nature. 314: 443-446. PMID: 2858819
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