Bafilomycin A1 A macrolide antibiotic and potent and selective inhibitor of vacuolar-type H+ ATPase (V-ATPase)

Bafilomycin A1 (CAS 88899-55-2)

Bafilomycin A1 | CAS 88899-55-2 is rated 4.8 out of 5 by 6.
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Synonym: Hygrolidin
Application: A macrolide antibiotic and potent and selective inhibitor of vacuolar-type H+ ATPase (V-ATPase)
CAS Number: 88899-55-2
Purity: ≥95%
Molecular Weight: 622.83
Molecular Formula: C35H58O9
* Refer to Certificate of Analysis for lot specific data (including water content).
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Bafilomycin A1 is a potent and selective inhibitor of V-ATPase (vacuolar-type H+ ATPase) and belongs to a class of membrane ATPase inhibitors. The compound can inhibit E1E2 ATPases from sarcoplasmic reticulum, Escherichia coli, and ox brain versus F1F0 ATPases found in bacteria and mitochondria. Some of the more bafilomycin A1 affected ATPases include Neurospora and plant vacuoles, as well as chromaffin granules. Additionally, Bafilomycin A1 prevents lysosomal cholesterol trafficking in macrophages and can be used to distinguish different types of ATPases. Research shows that Bafilomycin A1 can inhibit macroautophagy and promote apoptosis in colon cancer cells.


References

1. Hacke, W. 1978. EEG EMG Z Elektroenzephalogr Elektromyogr Verwandte Geb. 9: 179-181. PMID: 81128
2. Bowman, E.J., et al. 1988. Proc. Natl. Acad. Sci. U.S.A. 85: 7972-7976. PMID: 2973058
3. Furuchi, T., et al. 1993. J. Biol. Chem. 268: 27345-27348. PMID: 8262974
4. Wu, Y.C., et al. 2009. Biochem. Biophys. Res. Commun. 382: 451-456. PMID: 19289106

Usage :
Store, as supplied, at -20°C for up to 1 year. Store solutions at -20°C for up to 3 months.
Physical State :
Solid
Solubility :
Soluble in methanol, ethanol (0.1 mg/ml), DMSO (0.1 mg/ml), acetone, and chloroform. Insoluble in water.
Storage :
Store at -20° C
Boiling Point :
710.1-830.1° C at 760 mmHg (Predicted)
Density :
1.1 g/cm3 (Predicted)
Refractive Index :
n20D 1.53 (Predicted)
IC50 :
Adenosinetriphosphatase: IC50 = 30 nM (Gallus gallus); Vacuolar ATP synthase subunit S1: IC50 = 100 nM (human)
Ki Data :
vacuolar type H+ ATPase: Ki= 500 pM
pK Values :
pKa: 12.66 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
6436223
MDL Number :
MFCD06795130
Beilstein Registry :
4730700
SMILES :
C[C@H]1C/C(=C/C=C/[C@@H]([C@H](OC(=O)/C(=C/C(=C/[C@H]([C@H]1O)C)/C)/OC)[C@@H](C)[C@H]([C@H](C)[C@]2(C[C@H]([C@@H]([C@H](O2)C(C)C)C)O)O)O)OC)/C

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Certificate of Analysis

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Bafilomycin A1  Product Citations

See how others have used Bafilomycin A1. Click on the entry to view the PubMed entry .

Citations 1 to 10 of 74 total

PMID: # 28964970  Granato, M. et al. 2017. Cell. Signal. 40: 239-247.

PMID: # 28970837  Tseng, HHL. et al. 2017. Front Immunol. 8: 1161.

PMID: # 27670274  Zhao, Y. et al. 2017. Mol Nutr Food Res. 61:

PMID: # 28322744  Vervliet, T. et al. 2017. Biochem. Pharmacol. 132: 133-142.

PMID: # 28435516  D'Eliseo, D. et al. 2017. Genes Cancer. 8: 426-437.

PMID: # 29262591  Miranda-Gonçalves, V. et al. 2017. Oncotarget. 8: 103657-103670.

PMID: # 28667089  Molinski, SV. et al. 2017. EMBO Mol Med.

PMID: # 28454732  Yang, Z. et al. 2017. EBioMedicine. 19: 49-59.

PMID: # 28666573  Yoon, S. et al. 2017. Immunity. 47: 51-65.e7.

PMID: # 28521612  Law, KB. et al. 2017. Autophagy. 13: 868-884.

Citations 1 to 10 of 74 total

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. This chemical, Bafilomycin A1 (CAS 88899-55-2), will dissolve comfortably in DMSO and can be diluted to as low as 20% DMSO in water without problems.
Answered by: Chemical Support 1
Date published: 2017-02-28
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Rated 5 out of 5 by from NIce product from SCBT This V-ATPase inhibitor worked very well in the macrophages used in our paper. We used it to block macroautophagy. We got the desired results without problems.
Date published: 2018-10-25
Rated 4 out of 5 by from Work well to inhibit autophagy Used in HeLa cells to inhibit autophagosome and lysosome fusion. Western Blot detected significantly increased accumulation of LC3.
Date published: 2017-12-30
Rated 5 out of 5 by from Great antibody theses reagents worked well Great antibody theses reagents worked well
Date published: 2015-10-27
Rated 5 out of 5 by from No comment No comment
Date published: 2015-09-16
Rated 5 out of 5 by from Worked well in our experiments Worked well in our experiments, used this in plant cells.
Date published: 2015-09-14
Rated 5 out of 5 by from It works as I expected It works as I expected
Date published: 2015-04-10
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