Annexin V Binding Buffer is recommended for use with Annexin V reagents. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml. The binding buffer contains an optimal concentration of calcium which is required for Annexin V binding to phosphotidylserine (PS) on the cell surface.
Annexin V is a calcium dependent phospholipid binding
protein that preferentially binds to negatively charged phospholipids including PS. Cells progressing through apoptosis can be monitored according to their Annexin V and propidium iodide staining pattern. Early apoptotic cells will bind Annexin V but are not sensitive to intracellular staining with propidium iodide (PI). As cells progress through apoptosis the integrity of the plasma membrane is lost, allowing PI to penetrate and label the cells with a strong yellow-red fluorescence.
1. Vermes, I. 1995. J. Immunol. Methods. 184(1): 39-51. PMID: 7622868
Annexin V Binding Buffer is a optimal formula containing 2.5 mM Calcium Chloride in HEPES buffered saline. Cells should be resuspended in Annexin V Binding Buffer at a concentration 1x106/ml.
2.5 mM Calcium Chloride in HEPES buffered saline
Store at 4° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
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Annexin V Binding Buffer
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Various publications cite the use of AnnexinVarious publications cite the use of Annexin V Binding Buffer with Annexin V reagents. The binding buffer contains an optimal concentration of calcium which is required for Annexin V binding to phosphotidylserine (PS) on the cell surface. Cells progressing through apoptosis can be monitored according to their Annexin V and propidium iodide staining pattern. -SCBT Publication Review
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