AMPK alpha 1/2 Antibody (D-6) recommended for detection of AMPKα1 and AMPKα2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

AMPKα1/2 Antibody (D-6): sc-74461

AMPKα1/2 Antibody (D-6) is rated 4.6 out of 5 by 45.
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Datasheets
    • AMPKα1/2 Antibody (D-6) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 251-550 mapping at the C-terminus of AMPKα1 of human origin
    • recommended for detection of AMPKα1 and AMPKα2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including canine
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of AMPKα1/2 (D-6): sc-74461.
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When staining cells or tissue, should nuclear or cytoplasmic staining be expected?

Asked by: AbPolly
Thank you for your inquiry. For AMPK alpha 1 and AMPK alpha 2, both nuclear and cytoplasmic localizations have been observed. For your specific sample type, it is best to refer to literature.
Answered by: Technical Support 12
Date published: 2017-01-04
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Rated 4 out of 5 by from Very good antibody for western blotting Analyzed mice liver, and mice and humans leukocyte samples.
Date published: 2019-03-26
Rated 5 out of 5 by from sc-74461 sc-74461 works great for western blot with C57BL/6J mice.
Date published: 2018-11-23
Rated 4 out of 5 by from it can detect the signal For WB of Hela cell lysate, we can detect the right size band but with some other bands background.
Date published: 2018-08-22
Rated 4 out of 5 by from Works great developed nice clean Four star just because need to load more than the CST Ab (SCBT detects at 30ug while the CST work even at 15 or 20ug)
Date published: 2018-05-16
Rated 1 out of 5 by from poor antiboy for WB I bought this antibody several weeks ago and did western blot with total lysate of mouse heart tissue,. there is not any band .
Date published: 2018-01-22
Rated 5 out of 5 by from Get great band for WB I use 1:1000 dilute in WB, and it works well when detect PCs lysates
Date published: 2017-12-08
Rated 5 out of 5 by from results were excellent This is a great IHC staining antibody, 1/200 is very satisfactory in small intestine tissu of human.
Date published: 2017-10-26
Rated 5 out of 5 by from Very efficient AMPK&alpha1 antibody provides great staining in IF and IHC applications, binding is both strong and specific. We tried it detect this sample from dilution 1:800.
Date published: 2017-10-22
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