Ampicillin sodium salt, cell culture grade A widely used cell culture antibiotic

Ampicillin sodium salt, cell culture grade (CAS 69-52-3)

Ampicillin sodium salt, cell culture grade | CAS 69-52-3 is rated 5.0 out of 5 by 1.
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Synonym: D-(−)-α-Aminobenzylpenicillin ⋅ Na
Application: A widely used cell culture antibiotic
CAS Number: 69-52-3
Purity: ≥90%
Molecular Weight: 371.39
Molecular Formula: C16H18N3NaO4S
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ampicillin is a β-lactam antibiotic within the penicillin family. As a member of this family, Ampicillin is susceptible to β-lactamase, which hydrolyzes the β-lactam ring. This broad spectrum antibiotic is effective against gram-positive, gram-negative bacteria and anaerobic bacteria. Ampicillin is widely used in cell culture as a selective agent. As an antibiotic, Ampicillin binds to penicillin binding proteins (PBPs) on a susceptible organism and inhibits cell wall synthesis by interfering with the enzymes responsible for the formation of the organisms cell wall. After binding to the PBPs, Ampicilin acts as a structural analogue of acyl-D-alanyl-D-alanine, acylates the transpeptidase enzyme and thereby prevents the cross-linking of the peptidoglycan of the cell wall necessary for the growth of the bacterium.

Potency: ≥ 845 µg Ampicillin/mg (anhydrous)


References

1. Yang, W., et al. 2001. Protein Expr. Purif. 22: 472-478. PMID: 11483011
2. Rafailidis, P.I., et al. 2007. Drugs. 67: 1829-1849. PMID: 17722953
3. Joseleau-Petit, D., et al. 2007. J. Bacteriol. 189: 6512-6520. PMID: 17586646

Usage :
100 µg/ml for cell culture usage
Formulation :
≥ 845 µg Ampicillin/mg (anhydrous)
Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (50 mg/ml), chloroform, and acetone (1:50).
Storage :
Store at -20° C
Melting Point :
215° C
Refractive Index :
n20D 1.68 (Predicted)
IC50 :
Klebsiella pneumoniae: IC50 = 0.08 ug.mL-1; Staphylococcus aureus: IC50 = 0.74 ug.mL-1
Ki Data :
PEPT2: Ki= 1.3 mM (human); Oligopeptide transporter, kidney isoform: Ki= 1.3 mM (rat)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
2
RTECS :
XH8400000
PubChem CID :
23663979
Merck Index :
14: 586
MDL Number :
MFCD00064313
EC Number :
200-708-1
Beilstein Registry :
4119211
SMILES :
CC1([C@@H](N2[C@H](S1)[C@@H](C2=O)NC(=O)[C@@H](C3=CC=CC=C3)N)C(=O)[O-])C.[Na+]

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Certificate of Analysis

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Ampicillin sodium salt, cell culture grade  Product Citations

See how others have used Ampicillin sodium salt, cell culture grade. Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 19171936  Sato, I. et al. 2009. J. Biol. Chem. 284: 8042-8053.

PMID: # 11788606  Deneke, J. et al. 2002. J. Biol. Chem. 277: 10410-10419.

PMID: # 9374507  Scherzinger, E. et al. 1997. J. Biol. Chem. 272: 30228-30236.

PMID: # 7836390  Scheiffele, P. et al. 1995. J. Biol. Chem. 270: 1269-1276.

Citations 1 to 4 of 4 total
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Rated 5 out of 5 by from Various publications cite the use of Ampicillin Various publications cite the use of Ampicillin sodium salt (cell culture grade) in cell cultures as a selective agent. -SCBT Publication Review
Date published: 2015-03-09
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