Ammonium bicarbonate A buffer applications such as lyophilization and matrix assisted laser desorption

Ammonium bicarbonate (CAS 1066-33-7)

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Synonym: Ammonium hydrogen carbonate
Application: A buffer applications such as lyophilization and matrix assisted laser desorption
CAS Number: 1066-33-7
Purity: ≥99%
Molecular Weight: 79.06
Molecular Formula: NH4HCO3
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ammonium bicarbonate is a commonly used reagent for industrial and research procedures. Ammonium bicarbonate is volatile in solution and releases ammonia and CO2. This property makes ammonium bicarbonate a good buffer for such applications as lyophilization and matrix assisted laser desorption. Ammonium bicarbonate is also utilized for the in-gel digestion of proteins by trypsin and in the MALDI mass spectrometric analysis of proteins.


References

1. Li, S. H., et al., Synthesis of macroporous hydroxyapatite scaffolds for bone tissue engineering. J. Biomed. Mater. Res., 61(1), 109-120 (2002). 2. Navarro, J. M., et al., Ammonium, bicarbonate and calcium effects on tomato plants grown under saline conditions. Plant Sci., 157(1), 89-96 (2000). 3. Kuberan, B., et al., Preparation and isolation of neoglycoconjugates using biotin-streptavidin complexes. Glycoconj. J., 16(6), 271-281 (1999). 4. Wu, H., and Aboleneen, H., Sequencing oligonucleotides with blocked termini using exonuclease digestion and electrospray mass spectrometry. Anal. Biochem., 287(1), 126-135 (2000). 5. Li, G., et al., Rapid mass spectrometric identification of proteins from two-dimensional polyacrylamide gels after in gel proteolytic digestion. Electrophoresis, 18(3-4), 391-402 (1997).

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water (220 mg/ml at 20° C). Insoluble in alcohol, ethanol, benzene, and acetone.
Storage :
Store at room temperature
Melting Point :
60° C
Density :
1.58 g/cm3
Refractive Index :
n20D 1.54
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
BO8600000
PubChem CID :
14013
Merck Index :
14: 497
MDL Number :
MFCD00012138
EC Number :
213-911-5
Beilstein Registry :
4329606
SMILES :
C(=O)(O)[O-].[NH4+]

Download SDS (MSDS)

Certificate of Analysis

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