alpha-Adaptin 1/2 Antibody (C-8)recommended for detection of α-Adaptin 1 and α-Adaptin 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

α-Adaptin 1/2 Antibody (C-8): sc-17771

α-Adaptin 1/2 Antibody (C-8) is rated 4.9 out of 5 by 7.
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Datasheets
    • α-Adaptin 1/2 Antibody (C-8) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 678-977 (deletion at 707-728) of α-Adaptin 2 of mouse origin
    • recommended for detection of α-Adaptin 1 and α-Adaptin 2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of α-Adaptin 1/2 (C-8): sc-17771.
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Can α-Adaptin 1/2 (C-8): sc-17771 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, α-Adaptin 1/2 (C-8): sc-17771 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 8
Date published: 2017-02-07
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Rated 5 out of 5 by from Great antibody for WB Great antibody for western blot using this antibody for detect CASK in mouse brain lysate.
Date published: 2017-03-23
Rated 5 out of 5 by from Very popular choice! The (C-8) clone for alpha-Adaptin 1/2 is a well-established antibody with over a dozen known publications citing use of this clone.
Date published: 2017-01-21
Rated 5 out of 5 by from Strong band in WB in HepG2 Strong band in WB in HepG2, KNRK and NIH/3T3 whole cell lysates and mouse liver tissue extract. -SCBT QC
Date published: 2015-06-13
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic staining Excellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human adrenal gland tissue. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from no comment no comment
Date published: 2015-04-29
Rated 5 out of 5 by from Good specificity in WB in HepG2 whole cell lysate Good specificity in WB in HepG2 whole cell lysate. -SCBT QC
Date published: 2015-04-27
Rated 5 out of 5 by from Good publishable data in WB in human NIH/3T3 Good publishable data in WB in human NIH/3T3 and HeLaSS6. -SCBT Publication Review
Date published: 2015-02-07
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