alpha-Adaptin 1/2 Antibody (AP.6)recommended for detection of α-Adaptin 1 and α-Adaptin 2, as well as an alternatively spliced isoform of α-Adaptin 1 found in neurons of mouse, rat and human origin by WB, IP, IF and IHC(P)

α-Adaptin 1/2 Antibody (AP.6): sc-32284

α-Adaptin 1/2 Antibody (AP.6) is rated 4.0 out of 5 by 4.
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Datasheets
    • α-Adaptin 1/2 Antibody (AP.6) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against adaptor protein purified from bovine brain
    • recommended for detection of α-Adaptin 1 and α-Adaptin 2, as well as an alternatively spliced isoform of α-Adaptin 1 found in neurons of mouse, rat and human origin by WB, IP, IF and IHC(P); also reactive with additional species, including bovine
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • See α-Adaptin 1/2 (C-8): sc-17771 for α-Adaptin 1/2 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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I am using α-Adaptin 1/2 (AP.6): sc-32284 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Dr Ninau Qelp
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good specificity in WB in HepG2 whole cell lysates Good specificity in WB in HepG2 whole cell lysates. -SCBT QC
Date published: 2015-06-15
Rated 4 out of 5 by from Strong pulldown in IP in human YTS cells Strong pulldown in IP in human YTS cells. -SCBT Publication Review
Date published: 2015-05-02
Rated 4 out of 5 by from Strong staining in IF in human HeLa cells Strong staining in IF in human HeLa cells. -SCBT Publication Review
Date published: 2015-01-20
Rated 4 out of 5 by from Clear immunoperoxidase cytoplasmic staining Clear immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human cerebellum tissue. -SCBT QC
Date published: 2015-01-16
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