alpha-Actin Antibody (1A4)
  • α-Actin Antibody (1A4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
  • raised against an N-terminal decapeptide of α-Actin of human origin
  • recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)
  • available conjugated to agarose (sc-32251 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-32251 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-32251 PE), fluorescein (sc-32251 FITC), Alexa Fluor® 488 (sc-32251 AF488) or Alexa Fluor® 647 (sc-32251 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) (sc-516102), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
  • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of α-Actin (1A4): sc-32251.

α-Actin Antibody (1A4): sc-32251

α-Actin Antibody (1A4) is rated 4.7 out of 5 by 25.
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Datasheets
    • α-Actin Antibody (1A4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against an N-terminal decapeptide of α-Actin of human origin
    • recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)
    • available conjugated to agarose (sc-32251 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-32251 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-32251 PE), fluorescein (sc-32251 FITC), Alexa Fluor® 488 (sc-32251 AF488) or Alexa Fluor® 647 (sc-32251 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) (sc-516102), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Achieve better research results using superior monoclonal antibodies from Santa Cruz! Contact us to receive a FREE 10 µg sample of α-Actin (1A4): sc-32251.
Submit a review for this product and receive 15 CruzCredits

Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
α-Actin Antibody (1A4) sc-32251 200 µg/ml $279.00
α-Actin Antibody (1A4) AC sc-32251 AC 500 µg/ml, 25% ag $366.00
α-Actin Antibody (1A4) Alexa Fluor® 488 sc-32251 AF488 200 µg/ml $314.00
α-Actin Antibody (1A4) Alexa Fluor® 647 sc-32251 AF647 200 µg/ml $314.00
α-Actin Antibody (1A4) FITC sc-32251 FITC 200 µg/ml $292.00
α-Actin Antibody (1A4) HRP sc-32251 HRP 200 µg/ml $279.00
α-Actin Antibody (1A4) PE sc-32251 PE 200 µg/ml $303.00

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alpha-Actin Antibody (1A4) Product Citations

See how others have used alpha-Actin Antibody (1A4). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 128 total

PMID: # 28181406
Costa, JR. et al. 2017. Endocrine-disrupting effects of methylparaben on the adult gerbil prostate. Environ. Toxicol..

PMID: # 28843065
Li, R. et al. 2017. Self-assembled N-cadherin mimetic peptide hydrogels promote the chondrogenesis of mesenchymal stem cells through inhibition of canonical Wnt/β-catenin signaling. Biomaterials. 145: 33-43.

PMID: # 28899582
Dolivo, D.M. et al. 2017. FGF2-mediated attenuation of myofibroblast activation is modulated by distinct MAPK signaling pathways in human dermal fibroblasts. Journal of dermatological science.

PMID: # 27929729
Da Ros, M. et al. 2017. FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy. 13: 302-321.

PMID: # 28234994
Pan, X. et al. 2017. Mice, double deficient in lysosomal serine carboxypeptidases Scpep1 and Cathepsin A develop the hyperproliferative vesicular corneal dystrophy and hypertrophic skin thickenings. PLoS ONE. 12: e0172854.

PMID: # 28102743
Feng, D. et al. 2017. Expression and alteration of BKCa channels in the sphincter of Oddi's from rabbits with hypercholesterolemia. Channels (Austin). 1-9.

PMID: # 27959432
Cheng, GS. et al. 2017. Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells. Int. J. Mol. Med.. 39: 223-230.

PMID: # 26887982
Del Río, C. et al. 2016. The cannabinoid quinol VCE-004.8 alleviates bleomycin-induced scleroderma and exerts potent antifibrotic effects through peroxisome proliferator-activated receptor-γ and CB2 pathways. Sci Rep. 6: 21703.

PMID: # 27775075
Blomme, A. et al. 2016. Myoferlin regulates cellular lipid metabolism and promotes metastases in triple-negative breast cancer. Oncogene. 7: 41138.

PMID: # 27853137
Wu, X. et al. 2016. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis. Nat Commun. 7: 13498.

Citations 1 to 10 of 128 total

If I want to perform a Western blot using the α-Actin Antibody (1A4) on human samples, what secondary reagent should I use for detection?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for triple staining even if my other primary antibodies were also raised in mice?

Asked by: Trav11
When looking to perform triple staining with multiple mouse primary antibodies, we recommend using our directly conjugated mouse monoclonal primary antibodies. The α-Actin Antibody (1A4): sc-32251 is currently available conjugated to HRP (sc-32251 HRP), phycoerythrin (sc-32251 PE), fluorescein (sc-32251 FITC), Alexa Fluor® 488 (sc-32251 AF488) or Alexa Fluor® 647 (sc-32251 AF647).
Answered by: Tech Service 8
Date published: 2016-12-16

I would like to know whether this antibody is usable to evaluate the expression pattern of alfa-actin protein in adult zebrafish especially skeletal muscles. If not, could you please tell us the other useful antibodies?

Asked by: Yosuke
Thank you for your question. α-Actin Antibody (1A4): sc-32251 is recommended for detection of smooth muscle α-Actin, rather than skeletal muscle actin isoforms. This antibody has not yet been tested in zebrafish, and it is unknown if it would work for that species. As a recommended alternative, we would suggest our mouse monoclonal, β-Actin Antibody (C4): sc-47778. This antibody is recommended for detection of β-Actin; may cross-react with all six known isoforms of Actin (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform). This antibody has also been cited in the literature used in zebrafish in the following articles on NCBI's PubMed: PMID: 25075304, 22139372.
Answered by: Technical Support
Date published: 2016-12-02
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Rated 1 out of 5 by from Not well work in WB Too many unspecific WB band detected in human liver cancer BEL-7404 lysates. dilution ratio is 1:100
Date published: 2017-10-11
Rated 4 out of 5 by from works good on IF of tissue samples We used this product for the detection of a-SMA in the mouse fibrotic liver frozen sections.
Date published: 2017-10-04
Rated 5 out of 5 by from Good for WB, IF, FACS This antibody was used successfully for immunofluorescence (1:100), Western blot (1:500), and indirect flow cytometry (1:200). Good signal and specificity with very little background.
Date published: 2017-09-14
Rated 5 out of 5 by from Useful antibody! I bought this antibody a year ago and use it in WB analyse. This a-Actin antibody could specificly recognise a-SMA(a-smooth muscle actin).a-SMA is quite important to my study on liver fibrosis and hepatic stellate cells.In my experiment,I use the antibody to analyse a-SMA protein expression in cell extracts and mouse liver tissues.I incubated the PVDF membrane with a-actin antibody in a dilution of 1:1000 for a whole night. It is amazing!
Date published: 2017-09-01
Rated 5 out of 5 by from good antibody This is a trial product, which I was applied for and It's very great !
Date published: 2017-08-26
Rated 5 out of 5 by from good antibody This antibody shows a robust clear band and it is perfect
Date published: 2017-07-25
Rated 5 out of 5 by from WB analysis For mouse brain tissue analysis, there was band detected by a-actin antibody the molecular weight around 43KDa.No background .
Date published: 2017-07-08
Rated 5 out of 5 by from Very nice! 1:1000 diluted for wb of protein extracted from h9c2 cell,the band is so strong!
Date published: 2017-07-02
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