alpha-Actin Antibody (1A4)
  • α-Actin Antibody (1A4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
  • raised against an N-terminal decapeptide of α-Actin of human origin
  • recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)
  • available conjugated to agarose (sc-32251 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-32251 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-32251 PE), fluorescein (sc-32251 FITC), Alexa Fluor® 488 (sc-32251 AF488) or Alexa Fluor® 647 (sc-32251 AF647), 200 µg/ml, for IF, IHC(P) and FCM
  • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.

α-Actin Antibody (1A4): sc-32251

alpha-Actin Antibody (1A4) is rated 4.9 out of 5 by 15.
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Datasheets
    • α-Actin Antibody (1A4) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against an N-terminal decapeptide of α-Actin of human origin
    • recommended for detection of smooth muscle α-Actin of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; non cross-reactive with actin from fibroblasts (β-and γ-cytoplasmic), myocardium (α-myocardial), and striated muscle (α-sarcomeric)
    • available conjugated to agarose (sc-32251 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-32251 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-32251 PE), fluorescein (sc-32251 FITC), Alexa Fluor® 488 (sc-32251 AF488) or Alexa Fluor® 647 (sc-32251 AF647), 200 µg/ml, for IF, IHC(P) and FCM
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP): sc-516102, our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
α-Actin Antibody (1A4) sc-32251 200 µg/ml $279.00
α-Actin Antibody (1A4) AC sc-32251 AC 500 µg/ml, 25% ag $366.00
α-Actin Antibody (1A4) Alexa Fluor® 488 sc-32251 AF488 200 µg/ml $314.00
α-Actin Antibody (1A4) Alexa Fluor® 647 sc-32251 AF647 200 µg/ml $314.00
α-Actin Antibody (1A4) FITC sc-32251 FITC 200 µg/ml $292.00
α-Actin Antibody (1A4) HRP sc-32251 HRP 200 µg/ml $279.00
α-Actin Antibody (1A4) PE sc-32251 PE 200 µg/ml $303.00

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alpha-Actin Antibody (1A4) Product Citations

See how others have used alpha-Actin Antibody (1A4). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 110 total

PMID: # 28181406
Costa, JR. et al. 2017. Endocrine-disrupting effects of methylparaben on the adult gerbil prostate. Environ. Toxicol..

PMID: # 27929729
Da Ros, M. et al. 2017. FYCO1 and autophagy control the integrity of the haploid male germ cell-specific RNP granules. Autophagy. 13: 302-321.

PMID: # 28102743
Feng, D. et al. 2017. Expression and alteration of BKCa channels in the sphincter of Oddi's from rabbits with hypercholesterolemia. Channels (Austin). 1-9.

PMID: # 27959432
Cheng, GS. et al. 2017. Bone marrow-derived mesenchymal stem cells modified with IGFBP-3 inhibit the proliferation of pulmonary artery smooth muscle cells. Int. J. Mol. Med.. 39: 223-230.

PMID: # 26887982
Del Río, C. et al. 2016. The cannabinoid quinol VCE-004.8 alleviates bleomycin-induced scleroderma and exerts potent antifibrotic effects through peroxisome proliferator-activated receptor-γ and CB2 pathways. Sci Rep. 6: 21703.

PMID: # 27775075
Blomme, A. et al. 2016. Myoferlin regulates cellular lipid metabolism and promotes metastases in triple-negative breast cancer. Oncogene. 7: 41138.

PMID: # 27853137
Wu, X. et al. 2016. CUG-binding protein 1 regulates HSC activation and liver fibrogenesis. Nat Commun. 7: 13498.

PMID: # 26904289
Malashicheva, A. et al. 2016. Phenotypic and Functional Changes of Endothelial and Smooth Muscle Cells in Thoracic Aortic Aneurysms. Int J Vasc Med. 2016: 3107879.

PMID: # 25994834
Iannolo, G. et al. 2016. Numb Expression Contributes to the Maintenance of an Undifferentiated State in Human Epidermis. Cell Transplant. 25: 353-64.

PMID: # 27866874
Gong, H. et al. 2016. PAR1 Scaffolds TGFβRII to Downregulate TGF-β Signaling and Activate ESC Differentiation to Endothelial Cells. Stem Cell Reports. 7: 1050-1058.

Citations 1 to 10 of 110 total

If I want to perform a Western blot using the α-Actin Antibody (1A4) on human samples, what secondary reagent should I use for detection?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Tech Service 8
Date published: 2016-12-16

Can I use this mouse monoclonal antibody for triple staining even if my other primary antibodies were also raised in mice?

Asked by: Trav11
When looking to perform triple staining with multiple mouse primary antibodies, we recommend using our directly conjugated mouse monoclonal primary antibodies. The α-Actin Antibody (1A4): sc-32251 is currently available conjugated to HRP (sc-32251 HRP), phycoerythrin (sc-32251 PE), fluorescein (sc-32251 FITC), Alexa Fluor® 488 (sc-32251 AF488) or Alexa Fluor® 647 (sc-32251 AF647).
Answered by: Tech Service 8
Date published: 2016-12-16

I would like to know whether this antibody is usable to evaluate the expression pattern of alfa-actin protein in adult zebrafish especially skeletal muscles. If not, could you please tell us the other useful antibodies?

Asked by: Yosuke
Thank you for your question. α-Actin Antibody (1A4): sc-32251 is recommended for detection of smooth muscle α-Actin, rather than skeletal muscle actin isoforms. This antibody has not yet been tested in zebrafish, and it is unknown if it would work for that species. As a recommended alternative, we would suggest our mouse monoclonal, β-Actin Antibody (C4): sc-47778. This antibody is recommended for detection of β-Actin; may cross-react with all six known isoforms of Actin (including cytoplasmic β- and γ- Actin isoforms, skeletal, cardiac, and vascular α-Actin isoforms, and enteric γ-Actin isoform). This antibody has also been cited in the literature used in zebrafish in the following articles on NCBI's PubMed: PMID: 25075304, 22139372.
Answered by: Technical Support
Date published: 2016-12-02
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Rated 5 out of 5 by from The function of the antibody is ecxellent I applied the alpha-actin antibody sample two weeks ago, it has a strong signal, low non-specific background, I am very glad to have the opportunity to use this product.
Date published: 2017-04-06
Rated 5 out of 5 by from Great choice for alpha-Actin! This wonderful antibody is sure to produce the desired results in all standard applications. Reactivity with mouse, rat, and human gives it great versatility. Featured in over 100 reviews!
Date published: 2016-12-23
Rated 5 out of 5 by from Works great in WB Works great when detecting mouse kidney samples in WB, dilution 1:500. Clean blot and quite strong band of a-SMA (~42 kDa).
Date published: 2016-12-16
Rated 5 out of 5 by from Strong and specific antibody by WB I tested this antibody in the presence or absence of other actins and it seemed to specifically and strongly react with alpha-smooth muscle actin.
Date published: 2016-12-16
Rated 5 out of 5 by from Highly potent and specific antibody This monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was extremely strong detection of the ~43 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested.
Date published: 2016-11-10
Rated 5 out of 5 by from Good choice for wenster blot Showed distinct band at the indicated position and the band is very sharp and clear.
Date published: 2016-10-31
Rated 3 out of 5 by from
Date published: 2016-08-15
Rated 5 out of 5 by from I used this antibody for immunocytochemistry I used this antibody for immunocytochemistry (1:200 dilution) in A7r5 cells and got a great staining. works great for western blotting as well. I highly recommend this antibody for Immunocytochemistry and western blotting.Great product.<br />
Date published: 2015-10-29
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