ADH Antibody (G-7) recommended for detection of ADHα, ADHβ and ADHγ of human origin and Adh1 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA

ADH Antibody (G-7): sc-133207

ADH Antibody (G-7) is rated 3.5 out of 5 by 4.
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Datasheets
    • ADH Antibody (G-7) is a mouse monoclonal IgG2a (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1-300 mapping at the N-terminus of ADH of human origin
    • recommended for detection of ADHα, ADHβ and ADHγ of human origin and Adh1 of mouse and rat origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ADH (G-7): sc-133207.
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Can ADH (G-7): sc-133207 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Trav11
Thank you for your question. Yes, ADH (G-7): sc-133207 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-28
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Rated 4 out of 5 by from it works used it for western blotting on mouse liver, worked pretty well.
Date published: 2017-10-11
Rated 1 out of 5 by from Not suitable for IP when the antibody was used for immune precipitation, only a small amount of ADH1 was captured.
Date published: 2017-09-11
Rated 4 out of 5 by from Worked well with transfected lysate by WB Worked well with transfected lysate by WB. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from Produced superb immunoperoxidase nuclear Produced superb immunoperoxidase nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human ovary tissue. -SCBT QC
Date published: 2015-03-25
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