ACTL8 Antibody (A-4) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
specific for an epitope mapping between amino acids 339-365 at the C-terminus of ACTL8 of human origin
recommended for detection of ACTL8 of human origin by WB, IP, IF and ELISA
available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
blocking peptide, sc-377372 P
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ACTL8 (A-4): sc-377372.
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
ACTL8 (Actin-like protein 8), aslo known as CT57 (Cancer/testis antigen 57), is a member of the ARP family of actin-related proteins that contain an actin fold and are involved in spindle orientation, nuclear migration and chromatin remodeling events. Localized to the cytoplasm and expressed in the testis and pancreas, ACTL8 is 366 amino acids in length and is encoded by a gene that maps to human chromosome 1, which spans 260 million base pairs, contains over 3,000 genes and comprises nearly 8% of the human genome. Chromosome 1 houses a large number of disease-associated genes, including those that are involved in familial adenomatous polyposis, Stickler syndrome, Parkinson's disease, Gaucher disease, schizophrenia and Usher syndrome. Aberrations in chromosome 1 are found in a variety of cancers, including head and neck cancer, malignant melanoma and multiple myeloma.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What application is the blocking peptide sc-377372 P appropriate for?
Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 5 out of
the good designed for fluorescent visualAnti-ACTL8 Antibody at 1:1000 dilution + Hela whole cell lysateLysates/proteins at 20 μg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST,and it convent to diagnose.
Date published: 2017-02-11
Rated 5 out of
Produced positive Western blot data of ACTL8Produced positive Western blot data of ACTL8 expression in non-transfected and human ACTL8 transfected 293T whole cell lysates. -SCBT QC
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