ACTL8 Antibody (A-4) recommended for detection of ACTL8 of human origin by WB, IP, IF and ELISA

ACTL8 Antibody (A-4): sc-377372

ACTL8 Antibody (A-4) is rated 5.0 out of 5 by 2.
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Datasheets
    • ACTL8 Antibody (A-4) is a mouse monoclonal IgG2b (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 339-365 at the C-terminus of ACTL8 of human origin
    • recommended for detection of ACTL8 of human origin by WB, IP, IF and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-377372 P
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of ACTL8 (A-4): sc-377372.
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
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What application is the blocking peptide sc-377372 P appropriate for?

Asked by: TinTin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from the good designed for fluorescent visual Anti-ACTL8 Antibody at 1:1000 dilution + Hela whole cell lysateLysates/proteins at 20 μg per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST,and it convent to diagnose.
Date published: 2017-02-11
Rated 5 out of 5 by from Produced positive Western blot data of ACTL8 Produced positive Western blot data of ACTL8 expression in non-transfected and human ACTL8 transfected 293T whole cell lysates. -SCBT QC
Date published: 2014-07-02
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