AcrylamideA highly toxic, but commonly used matrix for electrophoretic separation of nucleic acids and proteins

Acrylamide (CAS 79-06-1)

Acrylamide | CAS 79-06-1 is rated 5.0 out of 5 by 1.
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Synonym: Acrylic acid amide; Propenamide
Application: A highly toxic, but commonly used matrix for electrophoretic separation of nucleic acids and proteins
CAS Number: 79-06-1
Purity: ≥98%
Molecular Weight: 71.08
Molecular Formula: C3H5NO
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Acrylamide is a commonly used matrix for electrophoretic separation of nucleic acids and proteins. Acrylamide forms linear polymers and bis-Acrylamide introduces cross-links between polyacrylamide chains. By mixing the appropriate ratio of Acrylamide to bis-Acrylamide, the optimal pore size can be obtained for the protein being resolved. To avoid the toxicity of dry acrylamide, ready-to-use acrylamide solutions are available. Acrylamide Solution 40% (sc-3721) and bis-Acrylamide 2% (sc-3719) solutions offer convenience and eliminate the risks associated with the handling of acrylamide powder. Polymerization agents, TEMED (sc-29111) and APS (Ammonium persulfate, sc-202946) are also available. For denaturing gels, SDS (20% Solution) (sc-24950) is available.


References

1. Raymond, S. and Aurell, B. 1962. Science. 138: 152-153. PMID: 17818402
2. Vesterberg, O. 1993. Electrophoresis. 14: 1243-1249. PMID: 8137783
3. Beeley, J.A., et al. 1996. Electrophoresis. 17: 505-506. PMID: 8740168
4. Langen, H., et al. 1997. Electrophoresis. 18: 2085-2090. PMID: 9420174
5. Molloy, M.P., et al. 1998. Electrophoresis. 19: 837-844. PMID: 9629924
6. Rabilloud, T. 2002. Proteomics. 2: 3-10. PMID: 11788986

Appearance :
powder
Physical State :
Solid
Storage :
Store at room temperature
Melting Point :
82-85° C
Boiling Point :
125° C at 25 mmHg
Density :
1.32 g/cm3
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
6579
SMILES :
C=CC(=O)N

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Rated 5 out of 5 by from Acrylamide is widely used to create a gel Acrylamide is widely used to create a gel used for separation of nucleic acids and proteins. It is primarily what is used in SDS-PAGE gels. The pore size can be altered by adjusting the ratio of acrylamide to bis-acrylamide. -SCBT QC
Date published: 2015-02-20
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