Ac-DEVD-pNAA colorimetric substrate for caspase-3 (CPP32) and related cysteine proteases

Ac-DEVD-pNA (CAS 189950-66-1)

Ac-DEVD-pNA | CAS 189950-66-1 is rated 5.0 out of 5 by 1.
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Synonym: N-Acetyl-Asp-Glu-Val-Asp p-nitroanilide; Caspase-3 Substrate (Chromogenic)
Application: A colorimetric substrate for caspase-3 (CPP32) and related cysteine proteases
CAS Number: 189950-66-1
Purity: ≥97%
Molecular Weight: 638.58
Molecular Formula: C26H34N6O13
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ac-DEVD-pNA is a colorimetric substrate for caspase-3 (CPP32) (Km=9.7μM) and related cysteine proteases. It is also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10. The sequence is based on PARP (poly(ADPribose) polymerase) cleavage site Asp216 for caspase-3. Cleavage is monitored colorimetrically at 405nm.


References

1. Lazebnik, Y.A., et al. 1994. Nature. 371: 346-347. PMID: 8090205
2. Nicholson, D.W., et al. 1995. Nature 376: 37-43. PMID: 7596430
3. Thornberry, N.A. 1994. Methods Enzymol. 244: 615-63. PMID: 7845238
4. Datta, R., et al. 1996. Blood. 88: 1936-1943. PMID: 8822910
5. Talanian, R.V., et al. 1997. J. Biol. Chem. 272: 9677-9682. PMID: 9092497
6. Datta, R., et al. 1997. J. Biol. Chem. 272: 1965-1969. PMID: 8999887

Physical State :
Solid
Sequence :
NAc-Asp-Glu-Val-Asp-pNA (pNA = p-Nitroaniline)
Solubility :
Soluble in water (partly), DMSO, buffer, pH 7, and acetonitrile:methanol (1:1) (1 mg/ml).
Storage :
Store at -20° C
Boiling Point :
1187.0±65.0° C at 760 mmHg (Predicted)
Density :
1.4±0.1 g/cm3 (Predicted)
Refractive Index :
n20D 1.59 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
4329
MDL Number :
MFCD00792707
SMILES :
CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC=C(C=C1)[N+](=O)[O-])NC(=O)C(CCC(=O)O)NC(=O)C(CC(=O)O)NC(=O)C

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Certificate of Analysis

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Ac-DEVD-pNA  Product Citations

See how others have used Ac-DEVD-pNA. Click on the entry to view the PubMed entry .

Citations 1 to 3 of 3 total

PMID: # 30795621  Nováková, J.|Talacko, P.|Novák, P.|Vališ, K.| et al. 2019. Cells. 8:

PMID: # 27161367  Zhao, JJ. et al. 2016. Neurochem. Res. 41: 1939-48.

PMID: # 26219933  Iloki-Assanga, SB. et al. 2015. BMC complementary and alternative medicine. 15: 254.

Citations 1 to 3 of 3 total

What is the solubility of this product?

Asked by: hawkeye11
This chemical, Ac-DEVD-pNA (CAS 189950-66-1), is sparingly soluble in water. It is recommended to prepare a stock solution in high purity DMSO (>99.9%) and then dilute to an aqueous buffer (pH 7) prior to use.
Answered by: Chemical Support 1
Date published: 2018-06-05
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Rated 5 out of 5 by from Various publications cite the use of Ac Various publications cite the use of Ac-DEVD-pNA, a colorimetric substrate, for the detection of caspase-3. It is also a substrate for caspase-6, caspase-7, caspase-8, and caspase-10 with cleavage being monitored colorimetrically at 405nm. -SCBT Publication Review
Date published: 2015-07-05
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