Ac-DEVD-AFC A fluorogenic substrate for caspase-3 and related cysteine proteases

Ac-DEVD-AFC (CAS 201608-14-2)

Ac-DEVD-AFC | CAS 201608-14-2 is rated 5.0 out of 5 by 1.
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Synonym: Caspase-3 Substrate (Fluorogenic)
Application: A fluorogenic substrate for caspase-3 and related cysteine proteases
CAS Number: 201608-14-2
Purity: ≥90%
Molecular Weight: 729.61
Molecular Formula: C30H34F3N5O13
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, which emits a blue to green shift in fluorescence. It also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10. It is known that Ac-DEVD-AFC has a greater Stokes' shift than Ac-DEVD-AMC once it has been cleaved. Since caspase-3 is an effector caspase, it is involved in the cleavage of many substrates which promote apoptosis. This indicator excites light at 400 nm and emits light at 505 nm. In addition, Ac-DEVD-AFC is modeled after Asp216 on caspase-3, the PARP cleavage site. Ac-DEVD-AFC is an essential indicator used to monitor the cellular effects of caspase-3.


References

1. Lazebnik, Y.A., et al. 1994. Nature. 371: 346-347. PMID: 8090205
2. Nicholson, D.W., et al. 1995. Nature 376: 37-43. PMID: 7596430
3. Xiang, J., et al. 1996. Proc. Natl. Acad. Sci. U.S.A. 93: 14559-14563. PMID: 896209
4 .Chandler, J.M., et al. 1998. J. Biol. Chem. 273: 10815-10818. PMID: 9556551
5. Marcelli, M., et al. 1998. Cancer Res. 58: 76-83. PMID: 9426061

Appearance :
Powder
Physical State :
Solid
Sequence :
Ac-Asp-Glu-Val-Asp-AFC
Solubility :
Soluble in 50% acetic acid in water (5 mg/ml), DMF, DMSO, methanol, and slightly soluble water (0.4 mg/ml).
Storage :
Store at -20° C
Boiling Point :
~1152.7° C at 760 mmHg (Predicted)
Density :
~1.5 g/cm3 (Predicted)
Refractive Index :
n20D 1.57 (Predicted)
Fluoresence :
λex 400 nm, λem 505 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
644234
MDL Number :
MFCD01310970
SMILES :
CC(C)[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)C

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Certificate of Analysis

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Ac-DEVD-AFC  Product Citations

See how others have used Ac-DEVD-AFC. Click on the entry to view the PubMed entry .

Citations 1 to 3 of 3 total

PMID: # 28981087  van Raam, BJ. et al. 2017. Cell Death Dis. 8: e3069.

PMID: # 27220334  Means, JC. et al. 2016. Neurochem. Res.

PMID: # 23303820  van Kruchten, R. et al. 2013. Blood. 121: 1850-7.

Citations 1 to 3 of 3 total
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Rated 5 out of 5 by from Many articles reported the usage of Ac Many articles reported the usage of Ac-DEVD-AFC, a fluorogenic subtrate of Caspase-3, to measure apoptosis and cell viability. -SCBT Publication Review
Date published: 2015-02-21
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