Ac-DEVD-AFC is a fluorogenic substrate for caspase-3 (CPP32) and related cysteine proteases, which emits a blue to green shift in fluorescence. It also acts as a substrate for granzyme B-processed caspase-6, caspase-7, caspase-8, and caspase-10. It is known that Ac-DEVD-AFC has a greater Stokes' shift than Ac-DEVD-AMC once it has been cleaved. Since caspase-3 is an effector caspase, it is involved in the cleavage of many substrates which promote apoptosis. This indicator excites light at 400 nm and emits light at 505 nm. In addition, Ac-DEVD-AFC is modeled after Asp216 on caspase-3, the PARP cleavage site. Ac-DEVD-AFC is an essential indicator used to monitor the cellular effects of caspase-3.
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See how others have used Ac-DEVD-AFC. Click on the entry to view the PubMed entry .
PMID: # 28981087 van Raam, BJ. et al. 2017. Cell Death Dis. 8: e3069.
PMID: # 27220334 Means, JC. et al. 2016. Neurochem. Res.
PMID: # 23303820 van Kruchten, R. et al. 2013. Blood. 121: 1850-7.
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