7-Aminoactinomycin D A fluorescent DNA dye for apoptosis and flow cytomtery studies

7-Aminoactinomycin D (CAS 7240-37-1)

7-Aminoactinomycin D | CAS 7240-37-1 is rated 5.0 out of 5 by 2.
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Synonym: 7-AAD
Application: A fluorescent DNA dye for apoptosis and flow cytomtery studies
CAS Number: 7240-37-1
Purity: ≥96%
Molecular Weight: 1270.43
Molecular Formula: C62H87N13O16
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
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7-Aminoactinomycin D (7-AAD), an analog of the antibiotic actinomycin D (sc-200906), is a fluorescent DNA dye which is useful for quantifying apoptosis. 7-AAD has also been used in studies on cellular necrosis, chromosome analysis and in cell cycle studies. As a fluorescent DNA dye, 7-AAD is able to intercalate DNA via binding to the GC regions. When used in flow cytometery, 7-AAD can be used to discriminate between live cells as well as early and late apoptosis. Additionally, 7-AAD can also be combined with other staining procedures for analysis of intracellular and cell surface antigens enabling multiparametric analysis. When utilizing 7-AAD with flow cytometery, early and late apoptosis can be studied by varying the concentration of 7-AAD. During apoptosis, the cellular plasma membrane is altered progressively, which allows the cell membrane to become permeable to 7-AAD. 7-AAD staining can also be adapted to include staining with other dyes for intracellular and cell surface antigens. 7-Aminoactinomycin D is an inhibitor of DNA Polymerase and RNA Polymerase.


References

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2. Toba, K., et al. 1992. Cytometry. 13: 60-67. PMID: 1372203
3. Schmid, I., et al. 1992. Cytometry. 13: 204-208. PMID: 1547670
4. Berg, R.J., et al. 1993. Carcinogenesis. 14: 103-106. PMID: 8425255
5. Schmid, I., et al. 1994. J. Immunol. Methods. 170: 145-157. PMID: 8157993
6. Lecoeur, H., et al. 2001. J. Immunol. Methods. 253: 177-187. PMID: 11384679

Physical State :
Solid
Solubility :
Soluble in DMSO (20 mg/ml), ethanol, chloroform (1 mg/ml), and dichloromethane (20 mg/ml).
Storage :
Store at -20° C
Melting Point :
252-253° C
Boiling Point :
~1400.8° C at 760 mmHg (Predicted)
Density :
~1.4 g/cm3 (Predicted)
Refractive Index :
n20D 1.66 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
AU1579000
Transport :
UN 3462, Class 6.1, Packing group II
PubChem CID :
16218991
Merck Index :
14: 2800
MDL Number :
MFCD00077295
Beilstein Registry :
5915844
SMILES :
C[C@@H]1[C@@H](C(=O)N[C@@H](C(=O)N2CCC[C@H]2C(=O)N(CC(=O)N([C@H](C(=O)O1)C(C)C)C)C)C(C)C)NC(=O)C3=CC(=C(C4=C3N=C5C(=C(C(=O)C(=C5O4)C)N)C(=O)N[C@H]6[C@H](OC(=O)C(N(C(=O)CN(C(=O)[C@@H]7CCCN7C(=O)[C@H](NC6=O)C(C)C)C)C)C(C)C)C)C)N

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7-Aminoactinomycin D  Product Citations

See how others have used 7-Aminoactinomycin D. Click on the entry to view the PubMed entry .

Citations 1 to 7 of 7 total

PMID: # 27062556  Blume-Peytavi, U. et al. 2016. J Eur Acad Dermatol Venereol. 30 Suppl 4: 3-56.

PMID: # 26676515  Woo, SM. et al. 2016. Mol Med Rep. 13: 1091-6.

PMID: # 25909288  Kim, JH. et al. 2015. Oncotarget. 6: 19528-41.

PMID: # 25486609  Falcón, CR. et al. 2014. PLoS ONE. 9: e114505.

PMID: # 22678910  Martínez, FF. et al. 2012. Eur. J. Immunol. 42: 1573-84.

PMID: # 22848374  Carranza, F. et al. 2012. PLoS ONE. 7: e40356.

PMID: # 20447697  Falcón, C. et al. 2010. Vet. Immunol. Immunopathol. 137: 36-46.

Citations 1 to 7 of 7 total
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Rated 5 out of 5 by from Worked well in application Worked well in application
Date published: 2015-10-09
Rated 5 out of 5 by from Graham JE; et al Graham JE; et al. (PubMed ID: 26000595) utilized 7-Aminoactinomycin D as a stain, to indicate necrosis or late apoptosis in RBCs stored in various solutions and concentrations. -SCBT Publication Review
Date published: 2015-04-04
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