60 kDa Ro/SSA Antibody (AA-3)recommended for detection of 60 kDa Ro/SSA of mouse, rat and human origin by WB, IP, IF and ELISA

60 kDa Ro/SSA Antibody (AA-3): sc-100844

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Datasheets
    • 60 kDa Ro/SSA Antibody (AA-3) is a mouse monoclonal IgG2a (kappa light chain) provided at 100 µg/ml
    • raised against a partial recombinant protein mapping within amino acids 1-101 of 60 kDa Ro/SSA of human origin
    • recommended for detection of 60 kDa Ro/SSA of mouse, rat and human origin by WB, IP, IF and ELISA
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of 60 kDa Ro/SSA (AA-3): sc-100844.
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Does this antibody have an associated blocking peptide?

Asked by: AbPolly
Thank you for your question. As this antibody was raised against a recombinant protein, it is not possible to offer a blocking peptide.
Answered by: Technical Support 6
Date published: 2017-01-11

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: AbPolly
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-11
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Rated 5 out of 5 by from Superb western and IF Very nice western blot and IF data; highly specific
Date published: 2017-01-15
Rated 5 out of 5 by from Works well in WB Good results in Western Blot with whole cell lysates and good staining in IF with PFA fixation.
Date published: 2016-12-23
Rated 4 out of 5 by from Clear immunofluorescence nuclear and cytoplasmic Clear immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-09
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