5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside (X-Gal) is a histochemical substrate for β-galactosidase. X-Gal is cleaved by β-galactosidase to yield an insoluble blue preceipitate. X-Gal is used to detect DNA insertions into the lacZ region of plasmid DNA, which causes the loss of β-galactosidase activity. Also used in conjunction with IPTG for detection of b-Galactosidase activity in bacterial colonies in a colorimetric assay.
Soluble in DMF (5% w/v), DMSO (20 mg/ml), and water (partly miscible).
Store at -20° C
236-238° C (dec.)
698.00° C at 760 mmHg (Predicted)
α20/D -60°, c = 1 in 50% DMF; α20/D -66°°, c = 1 in DMF
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Rated 5 out of
Various publications cite the use of 5Various publications cite the use of 5-Bromo-4-chloro-3-indolyl -D-galactopyranoside (X-Gal), a chromogenic histochemical substrate for -galactosidase, to detect DNA insertions into the lacZ region of plasmid DNA, which causes the loss of -galactosidase activity. X-Gal is cleaved by -galactosidase to yield an insoluble blue precipitate, it can also be used in conjunction with IPTG for detection of -Galactosidase activity in bacterial colonies in a colorimetric assay. -SCBT Publication Review
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