5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside A chromogenic histochemical substrate for β-galactosidase

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside (CAS 7240-90-6)

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside | CAS 7240-90-6 is rated 5.0 out of 5 by 1.
  • y_2019, m_2, d_16, h_2
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.11
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_280488, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getAggregateRating, 85ms
  • REVIEWS, PRODUCT
Synonym: X-Gal; BCIG; 5-Bromo-4-chloro-3-indolyl-β-D-galactoside; X Gal
Application: A chromogenic histochemical substrate for β-galactosidase
CAS Number: 7240-90-6
Purity: ≥99%
Molecular Weight: 408.63
Molecular Formula: C14H15BrClNO6
* Refer to Certificate of Analysis for lot specific data (including water content).

5-Bromo-4-chloro-3-indolyl b-D-galactopyranoside (X-Gal) is a histochemical substrate for β-galactosidase. X-Gal is cleaved by β-galactosidase to yield an insoluble blue preceipitate. X-Gal is used to detect DNA insertions into the lacZ region of plasmid DNA, which causes the loss of β-galactosidase activity. Also used in conjunction with IPTG for detection of b-Galactosidase activity in bacterial colonies in a colorimetric assay.

Appearance :
Crystalline powder
Physical State :
Solid
Solubility :
Soluble in DMF (5% w/v), DMSO (20 mg/ml), and water (partly miscible).
Storage :
Store at -20° C
Melting Point :
236-238° C (dec.)
Boiling Point :
698.00° C at 760 mmHg (Predicted)
Optical Activity :
α20/D -60°, c = 1 in 50% DMF; α20/D -66°°, c = 1 in DMF
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
65181
Merck Index :
14: 10074
MDL Number :
MFCD00005666
EC Number :
230-640-8
Beilstein Registry :
1402009
SMILES :
C1=CC(=C(C2=C1NC=C2O[C@H]3[C@@H]([C@H]([C@H]([C@H](O3)CO)O)O)O)Cl)Br

Download SDS (MSDS)

Certificate of Analysis

Adobe Acrobat Reader is required to reliably view,
print and comment on PDF documents

5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside  Product Citations

See how others have used 5-Bromo-4-chloro-3-indolyl beta-D-galactopyranoside. Click on the entry to view the PubMed entry .

Citations 1 to 2 of 2 total

PMID: # 28553941  Riglar, DT. et al. 2017. Nat. Biotechnol. 35: 653-658.

PMID: # 26133793  Deng, L. et al. 2015. Brain Res. 1622: 186-95.

Citations 1 to 2 of 2 total

What is the shelf life of compound in DMSO when stored at -20° C?

Asked by: Krishnanand
Thank you very much for your inquiry. Protected from light at -20 C, that solution can be stored for 6-12 months. If the solution turns pink, it should be discarded.
Answered by: Technical Support Europe
Date published: 2017-09-21

What is the expected color of this product?

Asked by: chemicalsmg
Thank you for your question. 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside: sc-280488 is expected to be white to off-white in color.
Answered by: Chemical Support 3
Date published: 2017-04-14
  • y_2019, m_2, d_16, h_2CST
  • bvseo_bulk, prod_bvqa, vn_bulk_2.0.11
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_2
  • loc_en_US, sid_280488, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getContent, 129ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Various publications cite the use of 5 Various publications cite the use of 5-Bromo-4-chloro-3-indolyl -D-galactopyranoside (X-Gal), a chromogenic histochemical substrate for -galactosidase, to detect DNA insertions into the lacZ region of plasmid DNA, which causes the loss of -galactosidase activity. X-Gal is cleaved by -galactosidase to yield an insoluble blue precipitate, it can also be used in conjunction with IPTG for detection of -Galactosidase activity in bacterial colonies in a colorimetric assay. -SCBT Publication Review
Date published: 2015-07-20
  • y_2019, m_2, d_16, h_2
  • bvseo_bulk, prod_bvrr, vn_bulk_2.0.11
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_1
  • loc_en_US, sid_280488, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-3.2.0
  • CLOUD, getReviews, 229ms
  • REVIEWS, PRODUCT

Santa Cruz Biotechnology, Inc. is a world leader in the development of products for the biomedical research market. Call us Toll Free at 1-800-457-3801.
Copyright © 2007-2019 Santa Cruz Biotechnology, Inc. All Rights Reserved. "Santa Cruz Biotechnology", and the Santa Cruz Biotechnology, Inc. logo, "Santa Cruz Animal Health", "San Juan Ranch", "Supplement of Champions", the San Juan Ranch logo, "Ultracruz", "Chemcruz", "Immunocruz", "Exactacruz", and "EZ Touch" are registered trademarks of Santa Cruz Biotechnology, Inc.
All trademarks are the property of their respective owners.