5-Azacytidine is a potent growth inhibitor and a cytotoxic agent. 5-Azacytidine acts as a demethylating agent by inhibiting DNA methyltransferase (Dnmt), forming covalent adducts with cellular DNMT1 depleting enzyme activity. 5-Azacytidine also improves the efficiency of reprogramming of stem cells and induces differentiation of mesenchymal stem cells into cardiomyocytes.
Kusaba, H., et al.: Eur. J. Biochem., 262, 924 (1999), Broday, L., et al.: Mol. Cell Biol., 19, 3198 (1999), Qian, X., et al.: Am. J. Pathol., 153, 1475 (1999), Canova, C., et al.: Mech. Ageing Dev., 101, 153 (1998)
Soluble in water (50 mg/ml), DMSO (49 mg/ml at 25° C), ethanol (~30 mg/ml), methanol, and DMF (~30 mg/ml).
I am using 5-Azacytidine in cell line . I prepared 100mM stocks in DMSO, and aliquoted them at -20C for a month . i have observed that the cells are not showing any response to the treatment. can I store the the 5-Aza in DMSO at -20C ?
Asked by: erfani 64
Thank you for your question. This can be stored in solvent for up to 2 years at -80C. For in vivo applications, add solvents to the product individually and in order, 5% DMSO + 30% PEG 300 + ddH20. For best results, use promptly after mixing.
Answered by: Technical Support
Date published: 2019-04-26
Hi. I want to know IC50 of 5- azacitidine for MOLT4 cell line treatment. Do you know it practically?
Rated 5 out of
Wu Z; et alWu Z; et al. (PubMed ID: 26403224) determined that 5-azacytidine, in combination with netropsin, increased and sustained the expression of CDKN2A, thus suppressing growth in KMT2A-AFF1-expressing cell lines. -SCBT Publication Review
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