4-Methylumbelliferyl β-D-N,N',N''-triacetylchitotrioside A fluorogenic substrate

4-Methylumbelliferyl β-D-N,N′,N″-triacetylchitotrioside (CAS 53643-13-3)

4-Methylumbelliferyl β-D-N,N′,NandPrime-triacetylchitotrioside | CAS 53643-13-3 is rated 5.0 out of 5 by 1.
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Synonym: 4-METHYL-7-COUMARINYL-TRI-N-ACETYL-BETA-CHITOTRIOSIDE
Application: A fluorogenic substrate
CAS Number: 53643-13-3
Molecular Weight: 785.75
Molecular Formula: C34H47N3O18
* Refer to Certificate of Analysis for lot specific data (including water content).
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4-Methylumbelliferyl β-D-N,N',N"-triacetylchitotrioside is a fluorogenic substrate that has been used for the detection of lysozyme, peptidoglycan, muramidase, and endochitinase activity. Cleavage of this substrate produces a fluorescent product, 4-methylumbelliferone (λex 360 nm, λem 455 nm).


References

1. Yang, Y. and Hamaguchi, K. 1980. J. Biochem. 87: 1003-1014. PMID: 7390976
2. Hollis, T., et al. 1997. Arch. Biochem. Biophys. 344: 335-342. PMID: 9264547
3. Howard, M.B., et al. 2003. J. Bacteriol. 185: 3352-3360. PMID: 12754233
4. Telkov, M.V., et al. 2006. Biochemistry Mosc. 71: 414-422. PMID: 16615861

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in DMSO (10-20 mg/ml), methanol: water (1: 1) (10 mg/ml), and pyridine: water (1: 1) (4.90-5.10 mg/ml).
Storage :
Store at -20° C
Boiling Point :
~1211.61° C at 760 mmHg (Predicted)
Density :
~1.6 g/cm3 (Predicted)
Fluoresence :
λex 360 nm, λem 455 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
16219671
MDL Number :
MFCD00036892
SMILES :
CC1=CC(=O)OC2=C1C=CC(=C2)OC3C(C(C(C(O3)CO)OC4C(C(C(C(O4)CO)OC5C(C(C(C(O5)CO)O)O)NC(=O)C)O)NC(=O)C)O)NC(=O)C

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Certificate of Analysis

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4-Methylumbelliferyl β-D-N,N',N''-triacetylchitotrioside  Product Citations

See how others have used 4-Methylumbelliferyl β-D-N,N',N''-triacetylchitotrioside. Click on the entry to view the PubMed entry .

Citations 1 to 1 of 1 total

PMID: # 21622555  Botelho, TO. et al. 2011. J. Biol. Chem. 286: 25697-25709.

Citations 1 to 1 of 1 total

What is the solubility of this product?

Asked by: hawkeye11
Thank you for your question. This product is soluble in DMSO (10-20 mg/ml), methanol: water (1: 1) (10 mg/ml), and pyridine: water (1: 1) (4.90-5.10 mg/ml). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-20
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Rated 5 out of 5 by from Chang Chang, et al. (PubMed ID 25437446) used 4-Methylumbelliferyl -D-N,N',N''-triacetylchitotrioside as a substrate in the process of purifying acidic chitinase from black soybean seeds (via ammonium sulfate fractionation and 3 successive steps of column chromatography). -SCBT Publication Review
Date published: 2015-02-27
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