3,3prime-Diaminobenzidine tetrahydrochloride A widely used chromogenic peroxidase substrate for staining

3,3′-Diaminobenzidine tetrahydrochloride  (CAS 868272-85-9)

3,3′-Diaminobenzidine tetrahydrochloride, CAS 868272-85-9 is rated 5.0 out of 5 by 1.
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| See product citations (23)
Synonym: 3,3',4,4'-Tetraaminobiphenyl tetrahydrochloride
Application: A widely used chromogenic peroxidase substrate for staining
CAS Number: 868272-85-9
Molecular Weight: 360.11
Molecular Formula: C12H14N44HCl
* Refer to Certificate of Analysis for lot specific data (including water content).
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Ordering Information

PRODUCT NAME CATALOG # UNIT PRICE QTY FAVORITES
3,3'-Diaminobenzidine tetrahydrochloride sc-209686 1 g $32.00
3,3'-Diaminobenzidine tetrahydrochloride sc-209686A 5 g $104.00

3,3'-Diaminobenzidine tetrahydrochloride hydrate is a water-soluble preparation of 3,3'-Diaminobenzidine, a tetraamine biphenyl compound used as a histological stain for peroxidase activity. Reaction between 3,3'-Diaminobenzidine and peroxidase forms a visible insoluble product. This approach has been used to assay activity of glutamate decarboxylase in rat cerebellar sections and to detect horseradish peroxidase presence on cultured cell surfaces.


References

1. Saito, K., et al. 1974. Proc. Natl. Acad. Sci. U.S.A. 71: 269-273. PMID: 4131274
2. Pelliniemi, L.J., et al. 1980. J. Histochem. Cytochem. 28: 191-192. PMID: 6986432
3. Messing, A., et al. 1985. J. Histochem. Cytochem. 33: 837-839. PMID: 3894500
4. de Jong, D., et al. 1994. Cancer Res. 54: 256-260. PMID: 8261449

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in water.
Storage :
Desiccate at room temperature
Melting Point :
280° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
23892
MDL Number :
MFCD08273058
EC Number :
231-018-9
SMILES :
C1=CC(=C(C=C1C2=CC(=C(C=C2)N)N)N)N.Cl.Cl.Cl.Cl

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Certificate of Analysis

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3,3prime-Diaminobenzidine tetrahydrochloride Product Citations

See how others have used 3,3prime-Diaminobenzidine tetrahydrochloride. Click on the entry to view the PubMed entry .

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Citations 1 to 10 of 23 total
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Rated 5 out of 5 by from Lukenda A; et al Lukenda A; et al., PubMed ID: 26290260 utilized 3,3'-Diaminobenzidine tetrahydrochloride as a chromogen to detect the expression of cancer stem cell markers CD117 (c-kit), CD34, CD20 and CD15. -SCBT Publication Review
Date published: 2015-03-28
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