2X Laemmli Sample Buffer

2X Laemmli Sample Buffer

2X Laemmli Sample Buffer is rated 4.5 out of 5 by 2.
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Application: For SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer
Supplemental Information: This is classified as a Dangerous Good for transport and may be subject to additional shipping charges.
* Refer to Certificate of Analysis for lot specific data (including water content).
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Laemmli sample buffer is especially formulated for protein sample preparation to be used in the Laemmli SDS-PAGE system. A protein sample is mixed with the 2X sample buffer (1:1) and heated in boiling water for 2-5 min. The 2-mercaptoethanol reduces the intra and inter-molecular disulfide bonds. The SDS detergent denatures the proteins and subunits and gives each an overall negative charge so that each will separate based on size. The bromophenol blue serves as a dye front that runs ahead of the proteins and also serves to make it easier to see the sample during loading. The glycerol increases the density of the sample so that it will layer in the sample well. Solution contains 4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.004% bromphenol blue and 0.125 M Tris HCl, pH approx. 6.8. Recommended usage: add 1 volume gel loading solution to 1 volume sample and mix well.


References

1. Laemmli, U.K. 1970. Nature. 227: 680-685. PMID: 5432063
2. Wu, M., et al. 2009. Diabetes Metab. Res. Rev. 25: 279-286. PMID: 19177471
3. Wu, M., et al. 2009. PLoS ONE. 4: e6430. PMID: 19641606

Formulation :
2X
Physical State :
Liquid
Solubility :
Soluble in water.
pH :
~6.8
Storage :
Store at -20° C
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
Transport :
UN 3082, Class 9, Packing group III

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Certificate of Analysis

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2X Laemmli Sample Buffer  Product Citations

See how others have used 2X Laemmli Sample Buffer. Click on the entry to view the PubMed entry .

Citations 1 to 3 of 3 total

PMID: # 26983346  Zhao, GX. et al. 2016. Biotech Histochem. 91: 269-76.

PMID: # 27100161  Choh, V. et al. 2016. Investigative ophthalmology & visual science. 57: 2140-51.

PMID: # 24629563  Yang, SJ. et al. 2014. Metab. Clin. Exp. 63: 693-701.

Citations 1 to 3 of 3 total
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Rated 4 out of 5 by from convenient Although, i may preparate this buffer ,but it need many time , meanwhile , the quality of my buffer can not identify. But , the 2X Laemmli Sample Buffer production was good. The price was not expensive. my job need it:)
Date published: 2016-12-03
Rated 5 out of 5 by from Numerous publications cite the use of Laemmli Numerous publications cite the use of Laemmli Sample Buffer in their protein studies, typically Western Blot applications. -SCBT Publication Review
Date published: 2015-01-19
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