15-LO2 Antibody (D-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
specific for an epitope mapping between amino acids 423-452 within an internal region of 15-LO2 of human origin
recommended for detection of 15-LO2 splice variants a, b and c of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
blocking peptide, sc-271290 P
See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of 15-LO2 (D-9): sc-271290.
Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
Lipoxygenases are a family of enzymes which dioxygenate unsaturated fatty acids, thus initiating lipoperoxidation of membranes, the synthesis of signalling molecules as well as inducing structural and metabolic changes in the cell. The Lox enzymes in mammals, 12-LO and 15-LO, are classified with respect to their positional specificity of the deoxygenation of their most common substrate, arachidonic acid. The metabolism of arachidonic acid leads to the generation of biologically active metabolites that have been implicated in cell growth and proliferation, as well as survival and apoptosis. 15-Lipoxygenase acts in physiological membrane remodeling and the pathogenesis of atherosclerosis, inflammation and carcinogenesis. It is highly regulated and expressed in a tissue- and cell-type-specific fashion. IL-4 and IL-13 play important roles in transactivating the 15-LO gene. Overexpression of 15-LO type 1 in prostate cancer contributes to the cancer progression by regulating IGF-1R expression and activation. 15-lipoxygenase, type II (15-LO2) is important for the conversion of arachidonic acid to 15S-hydroperoxyeicosatetraenoic acid. It is a cytoplasmic protein expressed primarily in cornea, lung, hair and prostate.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
What application is the blocking peptide sc-271290 P appropriate for?
Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here:
Rated 5 out of
it works well for WBthe expression of 15-lox-2 in Hek293 and hela cell transfected with 15-lox-2 Plasmid can be meassured by WB using 15-lo2 Ab with 1:100.
Date published: 2018-09-18
Rated 4 out of
Jen S from
Clean bands but two of them...I got this antibody as a free sample to detect endogenous 15-LO2 in human placenta samples. It has a clean band at the expected molecular weight of 75kda but it also has a distinct band around 55kDa, any idea of what is this second band? This second band is stronger in certain samples not all.
Date published: 2017-10-20
Rated 5 out of
Rana K Alfardan from
amazingused with cell line and animal tissue, dilution is 1:500
Date published: 2016-11-19
Rated 5 out of
Exceptional immunoperoxidase cytoplasmicExceptional immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human prostate tissue. -SCBT QC
Date published: 2015-04-19
Rated 5 out of
Nice Western Blot data of 15Nice Western Blot data of 15-LO2 expression in non-transfected and human 15-LO2 transfected 293T whole cell lysates. -SCBT QC
To place an order using RMB or to ship to mainland China, please visit www.scbio.cn
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