15-LO2 Antibody (D-9) recommended for detection of 15-LO2 splice variants a, b and c of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

15-LO2 Antibody (D-9): sc-271290

15-LO2 Antibody (D-9) is rated 4.8 out of 5 by 5.
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Datasheets
    • 15-LO2 Antibody (D-9) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 423-452 within an internal region of 15-LO2 of human origin
    • recommended for detection of 15-LO2 splice variants a, b and c of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-271290 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of 15-LO2 (D-9): sc-271290.
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What application is the blocking peptide sc-271290 P appropriate for?

Asked by: jerojero
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from it works well for WB the expression of 15-lox-2 in Hek293 and hela cell transfected with 15-lox-2 Plasmid can be meassured by WB using 15-lo2 Ab with 1:100.
Date published: 2018-09-18
Rated 4 out of 5 by from Clean bands but two of them... I got this antibody as a free sample to detect endogenous 15-LO2 in human placenta samples. It has a clean band at the expected molecular weight of 75kda but it also has a distinct band around 55kDa, any idea of what is this second band? This second band is stronger in certain samples not all.
Date published: 2017-10-20
Rated 5 out of 5 by from amazing used with cell line and animal tissue, dilution is 1:500
Date published: 2016-11-19
Rated 5 out of 5 by from Exceptional immunoperoxidase cytoplasmic Exceptional immunoperoxidase cytoplasmic and nuclear staining in formalin fixed, paraffin-embedded human prostate tissue. -SCBT QC
Date published: 2015-04-19
Rated 5 out of 5 by from Nice Western Blot data of 15 Nice Western Blot data of 15-LO2 expression in non-transfected and human 15-LO2 transfected 293T whole cell lysates. -SCBT QC
Date published: 2013-01-12
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