m-IgG kappa BP-CFL 594 mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Cruz Fluor™ 594, for WB (RGB), IF, IHC(P) and FCM

m-IgGκ BP-CFL 594: sc-516178

m-IgGκ BP-CFL 594 is rated 5.0 out of 5 by 11.
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Datasheets
    • mouse IgG kappa binding protein (m-IgGκ BP) conjugated to CruzFluor™ 594 (CFL 594)
    • supplied at 200 µg in 0.5 ml volume
    • CruzFluor™ 594 (CFL 594) is a red fluorescent dye that is an excellent substitute for Alexa Fluor® 594, but CruzFluor™, offering comparable photostability and the ability to resist protein quenching
    • Excitation of 587 nm, Emission of 603 nm
    • Suitable for use with RGB imaging systems, such as Invitrogen/iBright and other comparable systems
    • Strongly recommended alternative to conventional anti-mouse IgG secondary antibodies for RGB Western blotting (WB [RGB]), Immunofluorescence (IF) and Flow Cytometry (FCM)
    • Suitable for binding to mouse IgGκ light chain immunoglobulins, comprising approximately 98% of mouse monoclonal antibodies; not suitable for use with mouse monoclonal IgGλ light chain antibodies
    • Highly specific reagent that provides strong signal with minimal background and virtually complete elimination of lot to lot variation associated with conventionally generated secondary antibodies
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Rated 5 out of 5 by from Clear, bright staining Staining was clear and bright compared to the secondary I have been using for years. This worked better, so I think it is a great replacement! My results showed precise localization for the monoclonal antibodies I used.
Date published: 2016-12-05
Rated 5 out of 5 by from Great Products I have now tried several of the new binding proteins. I will definitely continue to use these. This product, along with the others, gave very specific cell staining results.
Date published: 2016-12-01
Rated 5 out of 5 by from New binding protein works really well !!!!!!!!!!!! This product worked better then our secondary antibody we have been using for years. Very good replacement. Our results using immunofluorescence staining protocols shows precise localization for regions of the cells expected to stain with our monoclonal antibody we developed. We are very happy with the outcome and look forward to trying out the other conjugated binding proteins. Love the product SCBT. Thank you.
Date published: 2016-11-30
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