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Platelets circulate in the blood as discoid cells which, when activated, change shape by polymerizing actin into several different structures, such as filopodia and stress fibers. Platelet activation is important for hemostasis. Kaptin (more commonly referred to as 2E4) binds to filamentous (F)-actin in an ATP-dependent manner and is involved in platelet activation. Kaptin is expressed in platelets, and localizes to the leading edge of the cells, supporting its involvement in the actin rearrangements that occur during activation. Kaptin is also present at the leading edge of the elongating stereocilium cells in the inner ear which play an integral role in the mechanotransduction of sound. Kaptin is involved in stereocilia formation, and may be an important factor in the development of DFNA4, a form of autosomal dominant non-syndromic hearing loss.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
I am using Kaptin (3B1): sc-100918 for immunofluorescence, what dilution of this antibody should I use?
Preguntado por: Dr Ninau Qelp
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here:
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