Santa Cruz Biotechnology now offers siRNA and shRNA (plasmid and lentiviral) gene silencers targeted to all (100%) known human and mouse genes. siRNAs are provided as pools of 3 target specific 19-25 nt siRNAs designed to knock down expression of specific gene of interest. Each vial contains 3.3 nmol of lyophilized siRNA and is sufficient for 50 - 100 transfections. For independent verification of gene silencing results, we also will provide, upon request, individual siRNA duplex components, each containing 3.3 nmol of lyophilized siRNA. shRNA Plasmid DNA Gene Silencers are provided as pools of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNA (sequences correspond to our well-established existing product line of siRNA gene silencers). shRNA Plasmids are provided as 20 µg transfection-ready plasmid DNA for either transient or stable (puromycin-selection) target specific gene silencing. shRNA Lentiviral Particles are provided as pools of three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin loop) shRNA and are provided as 200 µl transduction-ready viral particles for gene silencing in mammalian cells (human or mouse). Suitable control antibodies and RT-PCR primers are available for monitoring gene expression knockdown.
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