HRP and AP Substrates

We offer a broad range of HRP and AP Substrates for use in various immunoassays including detection of Horse Radish Peroxidase (HRP) and Alkaline Phosphatase (AP) conjugated secondaries used in Western Blotting, ImmunoHistoChemistry, ELISA, and other applications. Our high quality HRP and AP Substrates such as AEC (3-amino-9-ethylcarbazole), DAB (3-3 diaminobenzidine tetrachloride), and PNPP have been verified and tested for consistent results. Specialty formulas and ready to use products such as our BCIP/NBT and BCIP/IBT systems are also available. View detailed information on all our available HRP and AP Substrates by linking to the specific product blocks.

PRODUCT NAME/Application CAS Catalog # QUANTITY Price Citations RATING
PPHP
A peroxide used to assay peroxidase activity
87864-20-8 sc-204856
sc-204856A
500 µg
1 mg
$156.00
$296.00
0
Naphthol AS phosphate
An acid and alkaine phosphatase substrate
13989-98-5 sc-206000
sc-206000A
500 mg
1 g
$139.00
$241.00
4
Naphthol AS-MX phosphate disodium salt
A substrate for the histochemical demonstration of acid and alkaline phosphatase
96189-12-7 sc-215539
sc-215539A
100 mg
500 mg
$26.00
$75.00
1
Naphthol AS-MX phosphate disodium salt nonahydrate
A product used in histochemical detection of alkaline phosphatase
36889-52-8 sc-215540
1 g
$145.00
0
3,3prime-Diaminobenzidine
A peroxidase substrate and a reagent for spectrophotometric determination of selenium
91-95-2 sc-216567
sc-216567A
1 g
5 g
$25.00
$57.00
52
Naphthol AS-BI-phosphate, disodium salt
A substrate for the histochemical verification of ACP and alkaline phosphatase
530-79-0 sc-219334
sc-219334A
100 mg
500 mg
$43.00
$125.00
0
4-Aminophenylphosphate sodium salt
A substrate for the electrochemical measurement of alkaline phosphatase
sc-220931
10 mg
$33.00
0
5-Bromo-6-chloro-3-indolyl phosphate, p-toluidine salt
A staining substance for alkaline phosphatase activity
6769-80-8 sc-221017
sc-221017A
100 mg
250 mg
$250.00
$350.00
0
Naphthol AS-BI-phosphate
A substrate of ACP and Alkaline Phosphatase
1919-91-1 sc-222042
sc-222042A
100 mg
1 g
$34.00
$168.00
0
6-Chloro-3-indoxyl phosphate, p-toluidine salt
159954-33-3 sc-239096
sc-239096A
50 mg
100 mg
$58.00
$105.00
0
AEC, 50X
In the presence of horseradish peroxidase (HRP), produces a water-insoluble red oxidation product
132-32-1 sc-24979
10 ml
$170.00
16
BCIP/INT, 1X
A ready to use substrate for alkaline phosphate (AP) based detection systems. Suitable for IHC and ICC applications
sc-24980
100 ml
$80.00
7
Tetrazolium Violet
A reactive stain used for determining substrate catabolism in microbial cultures
1719-71-7 sc-206003
sc-206003A
1 g
5 g
$66.00
$265.00
0
BCIP/NBT Stock Solution, 50X
A substrate for alkaline phosphatase (AP) based detection systems including western blotting
sc-24981
8 ml
$155.00
30
DAB, 50X
A product used in HRP-based assays suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins
7411-49-6 sc-24982
sc-24982A
10 ml
100 ml
$75.00
$500.00
61
Naphthol AS-TR phosphate disodium salt
A substrate for the histochemical demonstration of acid and alkaline phosphatase
4264-93-1 sc-253179
sc-253179A
100 mg
1 g
$39.00
$111.00
0
Horseradish peroxidase (HRP), RZ 1.0
A heme-containing enzyme commonly used to oxidize a wide variety of substrates
9003-99-0 sc-280786
sc-280786A
100 mg
250 mg
$146.00
$275.00
6
3-Indoxyl phosphate p-toluidine salt
31699-61-3 sc-281357
sc-281357A
250 mg
500 mg
$162.00
$255.00
0
3-Indoxyl phosphate, bis(2-amino-2-methyl-1,3-propanediol) salt
107475-12-7 sc-281358
sc-281358A
100 mg
250 mg
$142.00
$250.00
0
2-Nitrophenyl b-D-galactopyranoside
A β-galactosidase substrate for colorimetric and EIA applications
369-07-3 sc-283249
sc-283249A
10 g
25 g
$210.00
$400.00
0
5-Bromo-3-indoxyl phosphate, p-toluidine salt
80008-69-1 sc-284557
sc-284557A
250 mg
500 mg
$192.00
$305.00
0
TMB Substrate Buffer, 1X
A single component, ready-to-use, working solution recommended for ELISA (microwell) procedures
sc-286967
100 ml
$45.00
1
ADHP
A fluorogenic substrate and stable hydrogen peroxide probe
119171-73-2 sc-291898
25 mg
$487.00
0
Naphthol AS phosphate disodium salt
An alkaline phosphatase histochemical substrate
69815-54-9 sc-206043
sc-206043A
500 mg
1 g
$97.00
$141.00
0
BCIP/NBT-Blue Liquid Substrate System for Membranes
A substrate for AP based detection systems including western blotting
sc-291929
100 ml
$88.00
12
pNPP Substrate Buffer
A ready-to-use diluent for PNPP substrate in ELISA procedures using alkaline phosphatase
sc-296099
500 ml
$65.00
1
BCIP-NBT Solution
A substrate for alkaline phosphatase (AP) based detection systems including western blotting
67-56-1 sc-358798
100 ml
$55.00
13
AEC Substrate Buffer, 10X
A 10X buffer diluent for use with AEC. For use in HRP-based detection systems
132-32-1 (solid) sc-36873
50 ml
$90.00
2
PNPP, Disodium Salt
An alkaline phosphatase substrate used in ELISA applications; absorbs light at 405nm
4264-83-9 sc-3720
sc-3720A
25 g
250 g
$75.00
$300.00
13
3-Indoxyl phosphate, disodium salt
A histochemical substrate for alkaline phosphatase
3318-43-2 sc-206651
sc-206651A
250 mg
1 g
$204.00
$578.00
0
5-Bromo-4-chloro-3-indoxyl phosphate, disodium salt
A histochemical substrate for alkaline phosphatase
102185-33-1 sc-207010
sc-207010A
25 mg
50 mg
$38.00
$42.00
0
BCIP p-toluidine salt
A histochemical substrate for alkaline phosphatase
6578-06-9 sc-207315
sc-207315A
100 mg
500 mg
$60.00
$190.00
0
Phenolphthalein monophosphate, bis(cyclohexylammonium) salt
An indicator for various titrations
14815-59-9 sc-208163
sc-208163A
1 g
5 g
$192.00
$575.00
0
3-Nitrotetrazolium blue chloride
An alkaline phosphatase substrate and free radical scavenging detector
127615-60-5 sc-209660
100 mg
$104.00
0
3,3'-Diaminobenzidine tetrahydrochloride
A widely used chromogenic peroxidase substrate for staining
868272-85-9 sc-209686
sc-209686A
1 g
5 g
$32.00
$104.00
22

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