Santa Cruz Biotechnology offers whole cell lysates for immunoprecipitation and are provided SDS-free. Each vial contains 2 mg protein in 800 µl SDS-free buffer. For subsequent WB use, resuspend pellet in 80-800 µl electrophoresis sample buffer (sc-24945), boil once prior to use and load up to 20 µl per lane. Lysates should be stored at -80° C and repeated freezing and thawing should be avoided.
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