Prepared Buffers

Santa Cruz Biotechnology now offers a broad range of convenient Prepared Buffers. The prepared buffers are formulated based on standard protocols, feature high-quality raw materials, and may be used in various immunoassays, including immunofluorescence, IHC, ELISA, and WB. Our popular PBS, TBS, and TBST buffers are offered in ready to use packets of the premixed ingredients, or in pre-filtered liquid formats. High quality acrylamides, APS, and TEMED are available for gel preparation. Proprietary formulas are also available, such as our Western Blot Stripping buffer which conserves loading proteins by allowing you to reprobe your gel multiple times with different antibodies. View detailed information on our available convenient Prepared Buffers by clicking on the product name.

PRODUCT NAME/Application CAS Catalog # QUANTITY Price Citations RATING
Plasmid Transfection Reagent
sc-108061
0.2 ml
$71.00
46
Plasmid Transfection Medium
sc-108062
20 ml
$8.00
31
Polybrene sup
sc-134220
10 mg/ml
$20.00
117
Formaldehyde
A 37% (saturated) solution stabilized with methanol
50-00-0 sc-203049
sc-203049A
sc-203049B
sc-203049C
25 ml
500 ml
1 L
4 L
$23.00
$49.00
$85.00
$170.00
0
Phosphomolybdic acid solution
Water soluble compound used in chromatography, histology staning and spectrophotometry
12026-57-2 sc-203346
sc-203346A
100 ml
500 ml
$51.00
$165.00
0
EDTA, 0.5 M, pH 8.0
A sterile-filtered solution of 0.5 M EDTA in H2O treated with DEPC, for use in molecular biology apps
6381-92-6 sc-203932
100 ml
$19.00
3
Glycogen
An inert carrier which significantly increases the efficiency of extracted DNA and RNA by ethanol precipitation
9005-79-2 sc-203976
1 ml
$103.00
0
Water, DEPC-Treated
Ready to use sterile filtered RNase free water for reconstitution of lyophilized products
7732-18-5 sc-204391
sc-204391A
1 L
10 x 50 ml
$26.00
$45.00
7
Glutaraldehyde solution, 50% w/w
An amine-reactive homobifunctional crosslinker
111-30-8 sc-207716
10 ml
$55.00
0
Ammonium hydroxide solution
A complexant and base for traditional qualitative inorganic analysis
1336-21-6 sc-214535
sc-214535A
500 ml
2.5 L
$37.00
$85.00
0
Potassium citrate tribasic solution
A buffering agent and chelator
866-84-2 sc-215738
sc-215738A
100 ml
500 ml
$42.00
$178.00
0
Potassium sodium tartrate solution
A chelator used for research purposes
304-59-6 sc-215742
sc-215742A
100 ml
500 ml
$52.00
$181.00
0
Triethylammonium Acetate Solution, HPLC
TEAA buffer, a 2M:2M concentrate in water
5204-74-0 sc-216105
sc-216105A
100 ml
500 ml
$216.00
$815.00
0
ddWater (endotoxin-free)
Sterile, double distilled endotoxin-free water
7732-18-5 sc-221533
1.5 ml
$35.00
0
Glucose solution
A biochemical used for research purposes
492-62-6 sc-221678
100 ml
$45.00
0
Blotto, non-fat dry milk
sc-2324
250 g
$36.00
35
TBS Blotto A
sc-2333
makes 500 ml
$26.00
32
TBS Blotto B
sc-2335
makes 500 ml
$26.00
7
Cacodylic acid
A useful arsinic acid for proteomics research
75-60-5 sc-239453
sc-239453A
5 g
10 g
$42.00
$75.00
0
Electrophoresis Sample Buffer, 2X
A ready-to-use reducing sample buffer for SDS-PAGE
sc-24945
sc-24945A
25 ml
1 L
$20.00
$500.00
30
Phosphate Buffered Saline: 1 L of 10X
a 10X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research
sc-24946
1 L
$36.00
2
Phosphate Buffered Saline: powder for 5 L of 10X
Ready to use for the preparation of 10X PBS; PBS solution is commonly used in biological research
sc-24947
1 EA
$85.00
1
RIPA Lysis Buffer System
A high quality, ready to use mammalian cell and tissue lysis buffer with protease inhibitors included
sc-24948
sc-24948A
50 ml
500 ml
$29.00
$169.00
905
Running Buffer, 10X
Tris-Glycine WB Running Buffer, pH 8.3; for sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE)
sc-24949
1 L
$32.00
1
SDS (20% Solution)
An anionic detergent effective in both ionic and anionic conditions
151-21-3 sc-24950
500 ml
$60.00
0
Tris Buffered Saline: 1 L of 10X
A convenient prepared buffer for use in various applications
sc-24951
1 L
$45.00
0
Tris Buffered Saline with Tween 20: 1 L of 10X
A convenient prepared buffer for use in various applications
sc-24953
1 L
$50.00
0
Towbin, with SDS, 10X
A western blot transfer buffer for use with nitrocellulose and PVDF transfer membranes, pH 8.3
sc-24954
1 L
$32.00
0
Towbin, without SDS, 10X
A western blot transfer buffer for nitrocellulose and PVDF membranes
sc-24955
1 L
$30.00
0
Water, Electrophoresis Grade
Purified water suitable for use in gel electrophoresis.
sc-24956
1 L
$12.00
0
DAB, 50X
A product used in HRP-based assays suitable for immunoblotting and immunohistochemical staining of nucleic acids and proteins
7411-49-6 sc-24982
sc-24982A
10 ml
100 ml
$75.00
$500.00
61
Triethylammonium bicarbonate buffer
A buffer for ion-exchange chromatography
15715-58-9 sc-253749
100 ml
$97.00
0
Glutaraldehyde solution, 70% w/w
A homobifunctional crosslinker suitable as a fixative for electron microscopy
111-30-8 sc-257558
10 ml
$46.00
0
Paraformaldehyde solution 4% in PBS
Commonly used to preserve tissues for routine histology
30525-89-4 sc-281692
1 L
$60.00
12
Tris Acetate-EDTA buffer, 50X
For DNA agarose gel electrophoresis; also used for non-denaturing RNA agarose gel electrophoresis
sc-281694
1 L
$68.00
0
Tris Buffered Saline with Tween 20: 1 L of 20X
A convenient prepared buffer for use in various applications
sc-281695
1 L
$65.00
0
Western Blot Stripping Buffer
breaks antibody-antigen interactions to allow membranes to be reprobed using different antibodies
sc-281698
500 ml
$95.00
1
Ethylenediaminetetraacetic acid diammonium copper salt solution
0.025 M in H2O, for complexometric titration with Cu-ISE
67989-88-2 sc-285567
250 ml
$29.00
0
Magnesium acetate solution
a biological buffer, ~1 M in H2O, pH 5.0-7.0 (25 °C)
142-72-3 sc-286142
sc-286142A
100 ml
500 ml
$34.00
$119.00
0
MOPS/EDTA Buffer, 10X Liquid Concentrate
A buffer for RNA electrophoresis in denaturing (formaldehyde) agarose gels
sc-286313
100 ml
$51.00
0
PBS (endotoxin-free)
Sterile and endotoxin-free PBS, isotonic and non-toxic to cells; for use in a wide variety of biological applications
sc-286634
1.5 ml
$40.00
3
Poly-L-Lysine Solution
An adhesive subbing solution for immunoperoxidase and routine histologic staining preparations
25988-63-0 sc-286689
100 ml
$147.00
2
HEPES Buffer Solution (1M)
A zwitterionic, all-purpose "Good's" buffer for use in biological research
7365-45-9 sc-286961
100 ml
$29.00
0
2X Laemmli Sample Buffer
For SDS-PAGE protein sample preparation, protein sample is mixed (1:1) with the 2X sample buffer
sc-286962
30 ml
$12.00
2
Laemmli Sample Buffer (odor-free), 2X
A sample buffer formulated for protein samples and used in the Laemmli SDS-PAGE system
sc-286963
5 ml
$12.00
1
TMB Substrate Buffer, 1X
A single component, ready-to-use, working solution recommended for ELISA (microwell) procedures
sc-286967
100 ml
$45.00
1
0.5M Sodium EDTA
A 0.2 µm filtered stock solution used routinely in buffers/solutions that require a metal chelator
sc-286969
1 L
$20.00
0
2,2prime-Azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)
ABTS, a peroxidase substrate suitable for use in ELISA procedures, Abs: 405 nm
28752-68-3 sc-288353
100 ml
$84.00
0
Ammonium acetate solution, 5M
An important reagent for studying molecular biology
631-61-8 sc-291892
sc-291892A
100 ml
250 ml
$71.00
$152.00
0
Ammonium chloride-ammonium hydroxide buffer solution
An alkaline buffer solution often used to assay water hardness
sc-291894
sc-291894A
500 ml
2 L
$26.00
$79.00
0
Ammonium formate solution
10 M, a buffer component for purification of glycosaminoglycans
540-69-2 sc-291895
sc-291895A
100 ml
500 ml
$73.00
$255.00
0
Ammonium phosphate monobasic solution
A buffer or buffer component used in solid phase column chromatography
7722-76-1 sc-291896
sc-291896A
100 ml
500 ml
$45.00
$182.00
0
Ammonium sulfate solution, 3.2 M
For the precipitation of proteins from crude serum sources
7783-20-2 sc-291897
sc-291897A
100 ml
500 ml
$15.00
$35.00
0
Annexin V Binding Buffer
A buffer used with Annexin V reagents that is used for flow cytometry and immunofluorescence applications
sc-291903
100 ml
$30.00
1
Azide, 2% solution
A preservative used in biologic solutions
26628-22-8 sc-291918
sc-291918A
5 ml
25 ml
$25.00
$95.00
0
Bicarbonate buffer solution
A sodium carbonate (Na2CO3)/sodium bicarbonate (NaHCO3) reagent used to maintain pH in serum samples
sc-291987
10 ml
$22.00
0
BICINE buffer Solution
1 M in H2O, N,N-Bis(2-hydroxyethyl)glycine
150-25-4 sc-291988
250 ml
$45.00
0
Buffer, reference standard, pH 10.00
A reference standard routinely used for the calibration of pH meters
sc-293966
500 ml
$29.00
0
Buffer, reference standard, pH 4.00
A compound routinely used for the calibration of pH meters at a pH of 4.00
sc-293967
500 ml
$28.00
0
Buffer, reference standard, pH 7.00
Color coded yellow, standard buffer solution routinely used for the calibration of pH meters
sc-293968
500 ml
$28.00
0
Carbonate/biCarbonate buffer packet
A diluent or dialysis buffer for proteins prior to conjugation of amine reactive tags
sc-293983
1 packet
$38.00
0
Cesium chloride Lanthanum chloride buffer
An additive for flame AAS determination of metals in a variety of samples
sc-293990
1 L
$65.00
0
Cibacron Blue Agarose
A reagent used in the isolation of enzymes requiring NAD<sup>+</sup> and NADP.
sc-294028
sc-294028A
1 ml
10 ml
$35.00
$150.00
0
Citrate Concentrated Solution
1M; may be used to prepare citrate buffer for antigen unmasking in IHC applications
68-04-2 sc-294091
250 ml
$31.00
1
Citrate-phosphate-dextrose solution
sc-294092
50 ml
$24.00
0
Citrate-phosphate-dextrose solution with adenine
An anticoagulating agent that contains adenine that is also used as a buffer
sc-294093
2 x 25 ml
$22.00
0
Formic acid solution
1.0 M in H2O, a volatile pH modifier in HPLC and CE; also a preservative and antibacterial agent
64-18-6 sc-294988
100 ml
$152.00
0
Gamma Binding Buffer
An optimal buffer for binding of desired IgG in affinity purification of antibodies via protein G agarose
sc-295001
1 L
$25.00
0
Glycine Buffer Solution, 100 mM, pH 2-2.5
For eluting antibodies in various antibody purification procedures, 0.2 &micro;m filtered
sc-295018
1 L
$20.00
0
siRNA Transfection Reagent
sc-29528
0.3 ml
$142.00
311
Lipoprotein Refolding Buffer
Specially formulated to provide optimum conditions for the refolding of denatured lipoproteins
sc-295364
sc-295364A
10 ml
50 ml
$40.00
$148.00
0
NaCl Solution, 1M
Stock solution used as a buffer in a wide variety
7647-14-5 (solid) sc-295832
1 L
$15.00
0
NaCl Solution, 5M
High-quality sodium chloride stock solution for the preparation of buffers used in molecular biology research
sc-295833
1 L
$28.00
0
PBS with 1M NaCl Solution
Used as a high salt cleaning solution for affinity purification of antibodies and buffer exchange protocols
sc-296027
1 L
$22.00
0
PBS with Azide
A preservative for biological solutions and excellent storage buffer; prevents microbial contamination
sc-296028
1 L
$25.00
1
pNPP Substrate Buffer
A ready-to-use diluent for PNPP substrate in ELISA procedures using alkaline phosphatase
sc-296099
500 ml
$65.00
1
RBC Lysis Buffer, 10X
A high quality reagent supplied as a 10X solution, yields a quantity sufficient to lyse up to 500 samples. pH 7.1-7.4
sc-296258
100 ml
$21.00
2
SAOB solution
A glycine-ascorbic acid based solution used to prevent the oxidation of sulfide in ion-selective electrode (ISE) assays
sc-296368
500 ml
$330.00
0
Sephadex Stripping/Regeneration Buffer
A buffer used to strip and regenerate sephadex columns for repeated use
sc-296378
1 L
$13.00
0
Sodium acetate buffer solution
3 M, non-sterile; 0.2 &micro;m filtered, may be used in a variety of molecular biology applications. pH 5.2&plusmn;0.1
sc-296389
sc-296389A
100 ml
500 ml
$18.00
$63.00
0
Sodium Acetate, 1M
Non-sterile; 0.2 &micro;m filtered NaOAc solution for use in a variety of applications; for example, protein gel stain prep.
sc-296390
sc-296390A
100 ml
500 ml
$25.00
$45.00
0
SSC Buffer, 20X Powder Pack
Each premixed pack makes 1L of 20X SSC Buffer (3M NaCl, 300mM Sodium Citrate)
sc-296419
2 pack
$31.00
0
STE buffer solution
A sodium Chloride-Tris-EDTA buffer formulated for DNA extraction protocols. pH 7.8&plusmn;0.2 (25 &deg;C)
sc-296422
sc-296422A
100 ml
500 ml
$45.00
$145.00
0
TG-SDS Buffer, 10X Powder Pack
Each pack makes 1 L of 10X Tris-Glycine-SDS Buffer concentrate for dilution to a 1X working solution
sc-296527
makes 1 L of 10X
$15.00
0
Total ionic strength adjustment buffer I
A high-quality reagent used for fluoride determination in presence of Ca, Mg, etc.
sc-296554
500 ml
$77.00
0
Total ionic strength adjustment buffer II
A high-quality reagent used for fluoride determination in presence of Fe(III) and Si
sc-296555
500 ml
$80.00
0
Trimethylammonium acetate solution
1M, high-quality buffer solution supplied at pH 6.5-7.5 (volatile)
6850-27-7 sc-296627
100 ml
$87.00
0
Trimethylammonium phosphate solution
MF: C3H12NO4P, MW: 157.1
sc-296628
100 ml
$98.00
0
Tris Acetate-EDTA buffer
Supplied as a ready to use 1X working solution used for DNA or non-denaturing RNA agarose gel electrophoresis
sc-296645
1 L
$19.00
0
Tris Acetate-EDTA buffer, 10X
Prepared with high-quality reagents for DNA or non-denaturing RNA agarose gel electrophoresis
sc-296647
sc-296647A
100 ml
1 L
$8.00
$35.00
0
TRIS Glycine buffer solution
10X, for electrophoretic transfer of protein from polyacrylamide gels to nitrocellulose sheets
sc-296648
1 L
$32.00
0
Tris solution, 1M, pH8
Routinely used to optimize antibody binding in the affinity purification of antibodies via Protein A agarose
sc-296649
1 L
$29.00
0
Tris-Borate-EDTA buffer, 10X
"TBE buffer", a concentrated solution ready to use for DNA and RNA polyacrylamide gel electrophoresis
sc-296650
sc-296650A
1 L
4 L
$29.00
$63.00
1
Tris-Borate-EDTA Buffer, 10X Powder Pack
A ready to mix buffer reagent used for DNA and RNA polyacrylamide gel electrophoresis
sc-296651
makes 1 L of 10X
$19.00
0
Tris-Borate-EDTA buffer, 5X
A concentrated solution ready to use for DNA and RNA polyacrylamide gel electrophoresis
610769-35-2 sc-296652
4 L
$47.00
0
Tris-EDTA buffer solution
Prepared with high-quality reagents and 0.2 &mu;m filtered. 10 mM Tris-HCl, 1 mM disodium EDTA, pH 8.0
sc-296653
sc-296653A
100 ml
500 ml
$15.00
$45.00
0
Tris-EDTA buffer solution, 100X
Used to solubilize and protect DNA and RNA
sc-296654
sc-296654A
100 ml
1 L
$15.00
$119.00
0
Tris-Phosphate-EDTA Buffer 10X
Concentrate for use in gel electrophoresis following dilution to working concentrations
sc-296655
1 L
$164.00
0
Urea, 2M
Stock solution used routinely as a cleaning and regeneration buffer for affinity columns
57-13-6 sc-296684
500 ml
$40.00
0
Glutaraldehyde solution, 25% w/w
A protein cross-linking agent
111-30-8 sc-300764
sc-300764A
100 ml
10 ml
$32.00
$20.00
0
HEPES sodium salt solution (1M)
An all-purpose buffer for use in biological research. 0.2 μm filtered
75277-39-3 sc-300789
sc-300789A
100 ml
500 ml
$22.00
$50.00
0
Imidazole buffer Solution
CAS: 288-32-4, MF: C3H4N2, MW: 68.08
288-32-4 sc-300829
sc-300829A
100 ml
500 ml
$48.00
$181.00
0
L-(+)-Lactic acid solution, 1M
An aqueous solution of 1M L-lactic acid in water. &le;1% D-lactic acid
79-33-4 sc-300859
1 L
$63.00
0
Magnesium formate solution
A high-quality 0.5 M solution in H2O used in cryopreservation of crystallized samples
6150-82-9 sc-300945
sc-300945A
100 ml
500 ml
$43.00
$161.00
0
MES solution
Used as a running buffer for resolving very small proteins
4432-31-9 sc-300959
100 ml
$105.00
0
MOPS solution
A morpholino propanesulfonic acid buffer
1132-61-2 sc-301170
sc-301170A
100 ml
500 ml
$30.00
$75.00
0
Nitric acid solution
A high-quality 0.1M solution often used as an electrolyte source
7697-37-2 sc-301479
1 L
$37.00
0
Ponceau S solution
A rapid protein band stain
6226-79-5 (solid) sc-301558
1 L
$78.00
4
Potassium acetate solution, 5M
A common laboratory buffer solution
127-08-2 sc-301582
sc-301582A
100 ml
250 ml
$79.00
$157.00
0
Potassium chloride solution, 1M
Used in a variety of biological applications, including DNA and RNA isolation
7447-40-7 sc-301585
500 ml
$20.00
0
Potassium formate solution
Supplied as a 14M solution in water for use in protein crystallization applications
590-29-4 sc-301590
sc-301590A
100 ml
500 ml
$55.00
$198.00
0
Potassium phosphate dibasic solution
A convenient 1M stock solution for making phosphate buffers
7758-11-4 sc-301600
1 L
$18.00
0
Potassium Phosphate Monobasic solution
A convenient 0.2 &micro;m filtered, 1M stock solution for making phosphate buffers
7778-77-0 sc-301601
1 L
$18.00
0
Propionic acid solution
An eluent and buffer component in chromatography applications
79-09-4 sc-301613
100 ml
$38.00
0
Sodium DL-lactate solution
For inclusion in standard lactated Ringer's solution
72-17-3 sc-301818
sc-301818A
sc-301818B
100 ml
500 ml
1 L
$10.00
$41.00
$78.00
0
Sodium hydroxide solution
CAS: 1310-73-2, MF: NaOH, MW: 40.00
1310-73-2 sc-301824
100 ml
$19.00
0
Sodium phosphate dibasic solution
For the preparation of phosphate buffers used in biological applications
7558-79-4 sc-301828
sc-301828A
100 ml
500 ml
$15.00
$37.00
0
Sodium phosphate monobasic solution
For the preparation of phosphate buffers used in biological applications
7558-80-7 sc-301829
sc-301829A
100 ml
500 ml
$35.00
$55.00
0
Sodium silicate solution
CAS: 84992-49-4, MF: Na2O(SiO2)x&bull;xH2O
84992-49-4 sc-301830
sc-301830A
25 ml
1 L
$29.00
$38.00
0
TAPS solution, 0.25M
Prepared with high-quality reagents and 0.2 &micro;m filtered, supplied at pH 9.1&plusmn;0.2
29915-38-6 (solid) sc-301862
250 ml
$37.00
0
TBS Powder Packet
A buffer used in some biochemical techniques for pH maintenance within a narrow range
sc-301863
1 packet
$44.00
0
TNT buffer solution
~pH 8, a high quality buffer reagent composed of TRIS, NaCl, and Tween 20
sc-301913
100 ml
$62.00
0
Tris Base solution
A high-quality 1.5 M buffer solution containing 0.05% sodium azide as a preservative
77-86-1 sc-301944
100 ml
$18.00
0
Tris citrate dibasic solution
a 1 M stock solution for use in protein isolation and purification applications
108321-34-2 sc-301945
250 ml
$91.00
0
Tris citrate monobasic solution
A high-quality 2M stock solution for use in protein purification procedures
108321-33-1 sc-301946
500 ml
$174.00
0
Tris citrate tribasic solution
1 M stock solution for use in protein purification and characterization applications
sc-301947
250 ml
$100.00
0
Tris hydrochloride solution, pH 7.2
Non-sterile; 0.2 &micro;m filtered, 1M stock solution used to make buffers
1185-53-1 (solid) sc-301949
1 L
$29.00
0
Tris hydrochloride solution, pH 7.4
Non-sterile; 0.2 &micro;m filtered, 1M stock solution used to make buffers
1185-53-1 (solid) sc-301950
1 L
$29.00
0
Tris hydrochloride solution, pH 7.6
Non-sterile; 0.2 &micro;m filtered, 1M stock solution used to make buffers
1185-53-1 (solid) sc-301951
1 L
$29.00
0
Tris hydrochloride solution, pH 7.8
Non-sterile; 0.2 &micro;m filtered, 1M stock solution used to make buffers
1185-53-1 (solid) sc-301952
1 L
$29.00
0
Tris hydrochloride solution, pH 8.0
Non-sterile; 0.2 &micro;m filtered, 1M stock solution used to make buffers
1185-53-1 (solid) sc-301953
1 L
$29.00
0
Dimethyl Sulfoxide (DMSO), cell culture reagent
A polar, aprotic organic solvent ideal for cryopreservation
67-68-5 sc-358801
100 ml
$30.00
2
EDTA, disodium salt, dihydrate, for molecular biology
A chelating agent and inhibitor of metalloproteases
6381-92-6 sc-359904
sc-359904A
50 g
250 g
$27.00
$92.00
0
Phosphate buffer, pH 7.2
A potassium phosphate solution prepared with high quality reagents; for use in biological applications
sc-360257
500 ml
$30.00
0
FCM Lysing solution
sc-3621
150 ml
$58.00
6
Phosphate Buffered Saline: 1 L of 1X
Ready to use 1X PBS solution frequently used as a wash and dilution buffer in biological research applications
sc-362182
1 L
$22.00
1
Phosphate Buffered Saline: 1 L of 20X
A 20X buffer solution ready for dilution to a 1X PBS working solution commonly used in biological research
sc-362183
1 L
$40.00
1
Tris Buffered Saline: 1 L of 1X
A ready to use convenient prepared buffer for use in various applications
sc-362185
1 L
$20.00
0