Vincristine Sulfate, a dimeric Vinca alkaloid, has been shown to bind to Tubulin via a protein self association reaction. This compound acts as a Tubuiln inhibitor by binding to sites at the ends of microtubules, which regulate the inhibition of Tubulin dimer addition to microtubule ends. Experiments have reported Vincristine Sulfate to demonstrate a capacity to suppress growth of proliferating cells through marked apoptosis and cell cycle arrest at G2/M. The cell death caused by this agent seems to result in a sustained accumulation of endogenous ceramide levels. Ceramide, has been proposed as a lipid second messenger with specific antiproliferative mediating responses. Vincristine Sulfate is an inhibitor of MAO. Vincristine Sulfate is also a substrate of PGP and CYP3A4.
|Appearance||White to off-white solid||White solid|
|Microanalysis||Elemental analysis within 0.4 of expected result for each element tested||Complies|
|Identification (1H-NMR)||Consistent with structure and shows < 1% of any other impurity||Complies|
|Identification (Mass Spectrometry)||Electrospray positive shows mass ion corresponding to [M-H2SO4+H]+ and [M-H2SO4+2H]2+||Mass ions: 825.5 and 413.5|
|Purity (HPLC)||> 98% by RP analysis||Complies|
|TLC||Single spot||Rf = 0.50|
|Solubility||Soluble in water to 100 mM||Complies|
NMR Conditions: 300 MHz in D2OTLC Conditions: PAW: BuOH = 2: 3; Visualized by UV and Ninhydrin
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