Nova-2 Double Nickase Plasmid (h): sc-404452-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Nova-2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Nova-2 Double Nickase Plasmid (h) and Nova-2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting NOVA2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Nova-2 Double Nickase Plasmid (h) | sc-404452-NIC | 20 µg | $410.00 | |||
Nova-2 Double Nickase Plasmid (h2) | sc-404452-NIC-2 | 20 µg | $410.00 |
NOVA2 encodes Nova-2, a neuron-enriched RNA-binding protein that recognizes YCAY motifs to control alternative splicing, mRNA stability, and localization of transcripts involved in synaptic function and neuronal maturation. Through its regulation of pre-mRNA processing, Nova-2 helps shape activity-dependent gene expression programs and contributes to the fidelity of axon guidance, synaptogenesis, and neurotransmission-related pathways. Perturbation of NOVA2-dependent splicing networks has been linked to neurodevelopmental phenotypes and altered neuronal circuit function, making NOVA2 a useful node for investigating RNA processing defects in nervous system biology. NOVA2 is also studied as part of broader splicing factor regulatory circuits that intersect with stress-responsive RNA metabolism and cell-type-specific transcriptome remodeling.
Nova-2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the NOVA2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within NOVA2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt NOVA2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of NOVA2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.