Date published: 2025-9-7

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MEK-1/2 Anticorpo (9G3): sc-81504

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Fichas de dados
  • MEK-1/2 Anticorpo 9G3é um anticorpo monoclonal produzido em camundongo IgG2a MEK-1/2 anticorpo, citado em 52 publicações, fornecido em 50 µg/0.5 ml
  • ativado contra o fosfopeptídeo correspondente ao loop de ativação de MEK-1/2 de human origem
  • recomendado para a detecção de the activation loop of MEK-1 and MEK-2 (independent of its phosphorylation status) de mouse, rat, human e canine origem em
  • See MEK-1 (H-8): sc-6250 for additional antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
  • Entre em contato conosco e receba GRATUITAMENTE um amostra de 10 µg de MEK-1/2 (9G3): sc-81504.
  • m-IgG Fc BP-HRP e 2a BP-HRP">m-IgG2a BP-HRP são os reagentes de deteção secundários preferidos para MEK-1/2 Antibody (9G3) para aplicações WB. Estes reagentes são agora oferecidos em conjuntos com MEK-1/2 Antibody (9G3)(ver informações de encomenda abaixo).

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VEJA TAMBÉM

O anticorpo MEK-1/2 (9G3) é um anticorpo MEK-1/2 monoclonal de camundongo IgG2a (também designado anticorpo MAP2K1, anticorpo MAP2K2 ou anticorpo de proteína quinase quinase ativada por mitógeno) que detecta a proteína MEK-1/2 de origem camundongo, rato, humana e canina por WB e IP. O anticorpo MEK-1/2 (9G3) está disponível como anticorpo anti-MEK-1/2 não conjugado. Foi identificada uma família de proteínas quinases localizada em um nível ascendente das MAP quinases e responsável por sua ativação. O membro protótipo dessa família, designado MAP kinase kinase, ou MEK-1, fosforila especificamente os resíduos de treonina e tirosina reguladores da MAP kinase presentes no motivo Thr-Glu-Tyr da ERK. Um segundo membro da família MEK, MEK-2, assemelha-se à MEK-1 em sua especificidade de substrato. A MEK-3 (ou MKK-3) funciona para ativar a p38 MAP kinase, e a MEK-4 (também chamada de SEK1 ou MKK-4) ativa as MAP kinases p38 e JNK. A MEK-5 tem o aspecto de fosforilar especificamente a ERK5, enquanto a MEK-6 fosforila a p38 e a p38b. A MEK-7 (ou MKK-7) fosforila e ativa a via de transdução de sinal JNK.

Para uso em exclusivo em pesquisa. Não se destina a uso em diagnostico e tratamento.

Informacoes sobre ordens

Nome do ProdutoNumero de CatalogoUNIDPrecoQdeFAVORITOS

MEK-1/2 Anticorpo (9G3)

sc-81504
50 µg/0.5 ml
$316.00

Pacote do MEK-1/2 (9G3): m-IgG Fc BP-HRP

sc-539619
50 µg Ab; 10 µg BP
$354.00

Pacote do MEK-1/2 (9G3): m-IgG2a BP-HRP

sc-546742
50 µg Ab; 10 µg BP
$354.00

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-02-17
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Rated 4 out of 5 by from Not very clear blotDetected the bands but the film was quite unclear. Incubated the antibody in 3% v/w milk.
Date published: 2021-03-17
Rated 2 out of 5 by from please use this antibody with caution!I have previously recommended this antibody. I have thought this antibody can equally recognise the phosphor- and non-phosphor-form of MEK-1/2, according to the statement "recommended for detection of the activation loop of MEK-1 and MEK-2 (independent of its phosphorylation status)". However, recently I did a growth factor stimulation experiment. I suddenly realised that MEK-1/2 Antibody (9G3) appears to have a strong preference for the p-MEK-1/2 over non-p-MEK-1/2, but is not totally clean with non-p-MEK-1/2. Thus, it would be problematic to use this antibody, especially when the phosphorylation level is low. So, please use this antibody with caution!
Date published: 2017-10-26
Rated 5 out of 5 by from great for WBThis ab has very good specificity and sensitivity for WB with MEK-1/2 from human, mouse and rat. The epitope is in the activation loop, for sequential WB using a phosphor-MEK-1/2 and this Ab, whether the binding of one ab will interfere with the binding of the other is not unknown.
Date published: 2017-01-30
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of MEK-1/2 expression in serum starved HeLa, Hep G2, HEK293, SH-SY5Y, MDCK, PC-12, CMT 93, Neuro 2A whole cell lysates.
Date published: 2017-01-20
Rated 5 out of 5 by from Published data for IP and WB in MCF7 breastPublished data for IP and WB in MCF7 breast cancer cells. -SCBT Publication Review
Date published: 2015-05-15
Rated 5 out of 5 by from Published data for WB in rat (VSMCs) vascularPublished data for WB in rat (VSMCs) vascular smooth muscle cells. -SCBT Publication Review
Date published: 2015-04-25
Rated 5 out of 5 by from Published data for IP in HEKPublished data for IP in HEK-293 cells. -SCBT Publication Review
Date published: 2015-03-13
Rated 5 out of 5 by from GoodGood, clear band visualized in WB in serum starved HeLa, Hep G2, HEK293, SH-SY5Y, MDCK, PC-12, CMT 93, Neuro 2A whole cell lysates. -SCBT QC
Date published: 2014-03-29
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MEK-1/2 Anticorpo (9G3) is rated 4.5 out of 5 by 8.
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