MBOAT2 CRISPR/Cas9 KO Plasmid (m): sc-426448
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- MBOAT2 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the MBOAT2 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
MBOAT2 CRISPR/Cas9 KO Plasmid (m) | sc-426448 | 20 µg | $397.00 |
Overview
Mboat2 encodes membrane bound O-acyltransferase domain containing 2 (MBOAT2), an ER- and membrane-associated acyltransferase implicated in remodeling phospholipids through lysophospholipid reacylation. By influencing fatty-acyl incorporation into membrane glycerophospholipids, MBOAT2 can affect membrane composition, organelle homeostasis, and lipid-dependent signaling processes linked to cell stress responses. Altered phospholipid metabolism is broadly relevant to metabolic dysfunction, inflammation, and cancer-associated membrane remodeling, making Mboat2 a useful entry point for mechanistic studies of lipid-driven phenotypes. In mouse systems, genetic perturbation of Mboat2 supports pathway-level analysis of acyltransferase networks and compensatory lipid remodeling.
MBOAT2 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Mboat2 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Mboat2 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Mboat2 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish MBOAT2 protein expression.
This CRISPR knockout system enables efficient generation of Mboat2-deficient cell models for investigation of MBOAT2 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Mboat2 exon(s) critical for MBOAT2 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Mboat2 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by MBOAT2 CRISPR/Cas9 KO Plasmid (m) and MBOAT2 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Mboat2 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by MBOAT2 HDR Plasmid (m) and MBOAT2 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Mboat2 homology arms to support homology-directed repair at defined Mboat2 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.