System includes 1.0 ml normal mouse blocking serum, 250 µg mouse monoclonal anti-rabbit IgG-Biotin secondary, avidin and biotinylated horseradish peroxidase (ABC reagents), peroxidase substrate, DAB chromogen and DAB dilution buffer.
Tissue sections are first incubated with primary antibody. Biotinylated secondary antibody is added, followed by the addition of preformed ABC reagent. The target protein is visualized by incubation in peroxidase substrate.
Number of slides: 200
While we continue to offer the ImmunoCruz® ABC Kit, we highly recommend using Direct HRP Conjugated Primary Monoclonal Antibodies for optimal IHC Staining. See the product page for antibody of interest for HRP Direct Conjugate antibody availability.