Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Histone Deacetylase 1 (HDAC1) Double Nickase Plasmid (m): sc-436647-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Histone Deacetylase 1 (HDAC1) Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Histone Deacetylase 1 (HDAC1) Double Nickase Plasmid (m) and Histone Deacetylase 1 (HDAC1) Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Hdac1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Histone Deacetylase 1 Antibody (10E2): sc-81598
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Histone Deacetylase 1 (HDAC1) Double Nickase Plasmid (m) | sc-436647-NIC | 20 µg | $410.00 | |||
Histone Deacetylase 1 (HDAC1) Double Nickase Plasmid (m2) | sc-436647-NIC-2 | 20 µg | $410.00 |
Mouse Hdac1 encodes histone deacetylase 1 (HDAC1), a class I HDAC that removes acetyl groups from lysine residues on histone tails to promote chromatin compaction and transcriptional repression. HDAC1 functions in multiprotein corepressor complexes such as Sin3, NuRD, and CoREST, integrating signals that regulate cell-cycle progression, DNA replication and repair, and lineage-specific differentiation programs. By coordinating epigenetic silencing across promoters and enhancers, HDAC1 helps maintain genome stability and shapes cellular responses to stress and developmental cues. Dysregulated HDAC1 activity is frequently associated with aberrant transcriptional programs observed in diverse disease-relevant models, including oncogenic transformation and neurodevelopmental phenotypes.
Histone Deacetylase 1 (HDAC1) Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Hdac1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Hdac1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Hdac1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Hdac1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.