PKC alpha Antibody (H-7)recommended for detection of PKC α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

PKC α Antibody (H-7): sc-8393

PKC α Antibody (H-7) is rated 4.7 out of 5 by 31.
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Datasheets

    • PKC α Antibody (H-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • specific for an epitope mapping between amino acids 651-672 at the C-terminus of PKC α of human origin
    • PKC alpha Antibody (H-7) is recommended for detection of PKC α of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • PKC alpha Antibody (H-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • blocking peptide, sc-8393 P
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of PKC α (H-7): sc-8393.
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Rated 5 out of 5 by from comletely worked Overexpressed HA-PKCa in 293 cells was strongly detected. There are no non-specific band. PKCb and d were not cross reacted. endogenous PKCa was detected in 293 cell lysates. 1/1000 dil in 2% BSA TBST, o/n reaction.
Date published: 2017-07-04
Rated 5 out of 5 by from Excellent for IHC on frozen sections We received a sample of this antibody. It worked very well for immunofluorescent staining on adult mouse eye cryosections, giving strong signals with minimal background.
Date published: 2017-06-29
Rated 5 out of 5 by from Great antibody Very strong signal at a 1:500 dilution in western blot.It gives a strong specific band at 80 kDa.
Date published: 2017-06-03
Rated 5 out of 5 by from Good results This antibody worked great for WB on human PBMC cell.
Date published: 2017-05-20
Rated 4 out of 5 by from worked for Western Blotting this antibody worked for western blotting in HepG2 lysate
Date published: 2017-05-11
Rated 5 out of 5 by from strong signal strong signal for WB, 1:1000 used,clear band and no background.
Date published: 2017-04-08
Rated 5 out of 5 by from Great antibody for WB I got strong band and clear background by using this antibody!
Date published: 2017-03-23
Rated 5 out of 5 by from Great Antibody for Flow Cytometry This antibody worked well for flow cytometry using SupT1 cells.
Date published: 2017-03-07
Rated 5 out of 5 by from Great result Great result detection for WB of human liver tissues,suggest use the dilution 1:300 to 1:800 .
Date published: 2017-02-26
Rated 5 out of 5 by from data in WB No better than this antibody for WB or staining, very good antibody, used on multiple cell lines and tissue types.
Date published: 2017-02-12
Rated 5 out of 5 by from The use of this antibody has good staining We used this antibody WB in mouse cells. Diluted 1:500. It has good staining at 80 kDa.
Date published: 2017-02-09
Rated 5 out of 5 by from Great for Western Blot This antibody produces a strong, reliable signal in Western blot on multiple cell lines and tissue types. Overall great antibody.
Date published: 2017-01-06
Rated 4 out of 5 by from works on macaque tissue antibody detects PKC-alpha in paraffin embedded macaque retina tissue when used at a dilution of 1:50 ON.
Date published: 2016-12-20
Rated 4 out of 5 by from Very good antibody for Western blotting This product has been independently tested and validated in-house at Kinexus Bioinformatics Corporation as a very potent and highly specific antibody for Western blotting with a mixture of mouse brain, testes and thymus tissue lysates. It detects an appropriately sized ~80 kDa target protein in immunoblots with medium to strong intensity.
Date published: 2016-10-13
Rated 5 out of 5 by from Very good in WB and IP Very good in WB and IP.
Date published: 2015-11-17
Rated 5 out of 5 by from We are happy with the products we buy often We are happy with the products we buy often!
Date published: 2015-09-17
Rated 5 out of 5 by from Works well for IF Works well for IF.
Date published: 2015-06-08
Rated 3 out of 5 by from Worked with transfected lysate by WB Worked with transfected lysate by WB -SCBT QC
Date published: 2015-04-01
Rated 5 out of 5 by from Great IF images strong cytoplasmic staining in K Great IF images strong cytoplasmic staining in K-562 samples. -SCBT QC
Date published: 2015-03-03
Rated 5 out of 5 by from Good cytoplasmic staining with paraffin Good cytoplasmic staining with paraffin-embedded human adrenal gland samples. -SCBT QC
Date published: 2015-02-27
Rated 5 out of 5 by from Robust staining in paraffin Robust staining in paraffin-embedded human colon tumor. -SCBT QC
Date published: 2015-02-25
Rated 5 out of 5 by from Good Western blot detection of FADD phosphorylation Good Western blot detection of FADD phosphorylation using A-431 cells. -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Positive Western blot analysis of PKC expression Positive Western blot analysis of PKC expression in NIH/3T3 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-01-17
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