F4/80 Antibody (C-7)recommended for detection of EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog by WB, IP, IF, IHC(P) and ELISA

F4/80 Antibody (C-7): sc-377009

F4/80 Antibody (C-7) is rated 4.4 out of 5 by 20.
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Datasheets
    • F4/80 Antibody (C-7) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 335-634 mapping within an extracellular domain of F4/80 of mouse origin
    • F4/80 Antibody (C-7) is recommended for detection of EMR1 of human origin, F4/80 of mouse origin and the corresponding rat homolog by WB, IP, IF, IHC(P) and ELISA
    • F4/80 Antibody (C-7) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of F4/80 (C-7): sc-377009.
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Rated 4 out of 5 by from WB This antibody could be used to do WB, but not for IHC-P.
Date published: 2017-04-06
Rated 5 out of 5 by from Great !!! The antibody worked well on Western Blotting and IHC.
Date published: 2017-03-22
Rated 5 out of 5 by from Works great in Mouse! I asked for a sample because we tried this target from 4 other companies and could not get it to work. The first run worked with mouse spleen. We have to make small modifications to the stain still but first run is very nice as a start. Very easy to work up and the best price around!
Date published: 2017-02-15
Rated 5 out of 5 by from An automatic choice for immunostaining The only antibody you should choose if you plan on doing any stains. It has great specificity and will give you a clear bright signal. Has nearly 10 citations to its name.
Date published: 2017-01-06
Rated 5 out of 5 by from Superb!! Nice product for both western and ihc applications
Date published: 2017-01-05
Rated 5 out of 5 by from Works well. Used it for IF (Frozen section )at a dilution of 1:100,works well.
Date published: 2016-12-22
Rated 5 out of 5 by from Strong cytoplasmic staining in IHC Strong cytoplasmic staining in IHC in human transfected NIH-3T3 cells.
Date published: 2016-11-29
Rated 5 out of 5 by from Good for immunofluorescence staining Use for immunofluorescence staining in mouse esophagus cancer tissue, the signal was great.
Date published: 2016-11-14
Rated 1 out of 5 by from We used a MOM (mouse on mouse kit) since We used a MOM (mouse on mouse kit) since we ran this F4/80 on mouse tissue. We did a titer assay on tissue we know is positive for F4/80 and those slides showed no positive staining.
Date published: 2015-03-24
Rated 5 out of 5 by from Positive WB detection in WEHI Positive WB detection in WEHI-3 and M1 whole cell lysates -SCBT QC
Date published: 2015-03-11
Rated 5 out of 5 by from Immunofluorescence staining of methanol Immunofluorescence staining of methanol-fixed NIH/3T3 cells showed cytoplasmic localization -SCBT QC
Date published: 2015-02-04
Rated 5 out of 5 by from Immunoperoxidase staining of formalin fixed Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showed cytoplasmic staining of respiratory epithelial cells -SCBT QC
Date published: 2013-12-22
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