EB1 Antibody (1A11/4) recommended for detection of EB1 of human, canine and monkey origin by WB, IP, IF and IHC(P)

EB1 Antibody (1A11/4): sc-47704

EB1 Antibody (1A11/4) is rated 4.9 out of 5 by 11.
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Datasheets

    • EB1 Antibody (1A11/4) is a mouse monoclonal IgG2b (kappa light chain) EB1 antibody provided at 200 µg/ml
    • raised against EB1 δC1 mutant
    • EB1 Antibody (1A11/4) is recommended for detection of EB1 of human, canine and monkey origin by WB, IP, IF and IHC(P)
    • EB1 Antibody (1A11/4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of EB1 (1A11/4): sc-47704.
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Can EB1 (1A11/4): sc-47704 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, EB1 (1A11/4): sc-47704 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent! Great antibody for WB and IF on Jurkat T cells. Couldn't be better.
Date published: 2019-01-22
Rated 5 out of 5 by from Works very well in Immunofluorescence Good signal in RPE-1 cells, MetOH fixation, Can be used to distinguish dynamic microtubules.
Date published: 2018-03-27
Rated 4 out of 5 by from Good for IF This EB1 Antibody (1A11/4) shows strong specific signal in IF staining in primary rat motor neuron.
Date published: 2017-12-04
Rated 5 out of 5 by from good antibody work well in Hela A549 and MCF7 cellyste at the dilution of 1:100
Date published: 2017-10-24
Rated 5 out of 5 by from Great antibody for WB! It works really well in WB. Dilution 1:3000. The cell extracts were from human colon carcinoma cells.
Date published: 2017-10-19
Rated 5 out of 5 by from Fantastic EB1 Antibody! Great performance seen in Western Blot, IF and IHC. Works with paraffin-embedded sections. No additional bands in WB!
Date published: 2017-01-20
Rated 5 out of 5 by from Works well in IF Works well in IF, can publish with it.
Date published: 2015-09-29
Rated 5 out of 5 by from Good publishable data in WB and siRNA in Good publishable data in WB and siRNA in human cervical cancer SiHa, HeLa & CaSki cell line. -SCBT Publication Review
Date published: 2015-06-03
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