Dnmt1 Antibody (H-12)recommended for detection of Dnmt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA

Dnmt1 Antibody (H-12): sc-271729

Dnmt1 Antibody (H-12) is rated 4.4 out of 5 by 21.
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Datasheets

    • Dnmt1 Antibody (H-12) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against amino acids 1317-1616 mapping near the C-terminus of Dnmt1 of human origin
    • Dnmt1 Antibody (H-12) is recommended for detection of Dnmt1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
    • Dnmt1 Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of Dnmt1 (H-12): sc-271729.
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Rated 5 out of 5 by from Dnmt1 antibody Dnmt1 antibody in IF and got good staining, happy with purchase.
Date published: 2017-05-10
Rated 5 out of 5 by from solid choice Antibody has good quality in Western blot, it works well.
Date published: 2017-04-10
Rated 4 out of 5 by from good signal Using ECL detection system I got good bands in the specific molecular size in glioblastoma cell lines. However, a strong inespecific band around 37 KDa was also observed.
Date published: 2017-03-10
Rated 5 out of 5 by from Dnmt1抗体很好用 我们实验室使用了这个抗体的小样作免疫荧光实验,实验效果很好,特异性高,背景很干净,基本没有其他非特异荧光
Date published: 2017-02-28
Rated 5 out of 5 by from The effect is very good. The result of Dnmt1 antibody satisfied us. Staining data very strong, good specificity.
Date published: 2017-02-07
Rated 1 out of 5 by from Review of anti-DNMT1 (H-12) antibody An anti-DNMT1 (H-12) antibodies sample was kindly gifted by Santa Cruz Biotechnology. I used them to detect DNMT1 protein in porcine corpus luteum protein lysates. Additionally I loaded protein lysates from human endometrial adenocarcinoma (Ishikawa) cell line and from human trophoblast (HTR8/SV-neo) cell line. The amount of protein loaded on gel was 60 ug. The antibodies were used in 1:100. Signal was detected via chemiluminescence using Bio-Rad equiplemt and reagents.There was no signal in porcine tissues whereas in human lysates I was able to detect bands. Maybe antibodies used in 1:50 would work better however, the amount of requested sample was not enough to check it.
Date published: 2017-01-24
Rated 5 out of 5 by from Outstanding product Great western blot and IHC results;best product!!!
Date published: 2017-01-06
Rated 5 out of 5 by from Good detection by WB Single, specific band observed in human Jukart cells.
Date published: 2016-12-23
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal with human H157 and LTE cells. -SCBT Publication Review
Date published: 2015-04-20
Rated 5 out of 5 by from Good WB publishable data gathered with rat Good WB publishable data gathered with rat Kupffer cells. -SCBT Publication Review
Date published: 2015-03-30
Rated 5 out of 5 by from WB Published with this antibody using human BGC WB Published with this antibody using human BGC-823, ACG and GES-1 gastric cancer cell line for immunoblot analysis -SCBT Publication Review
Date published: 2015-02-20
Rated 5 out of 5 by from Produced great immunoperoxidase nuclear staining Produced great immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human lymph node tissue. -SCBT QC
Date published: 2015-01-28
Rated 5 out of 5 by from WB positive with human THP WB positive with human THP-1 monocytes. -SCBT Publication Review
Date published: 2015-01-12
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