4E-BP1 Antibody (P-1)recommended for detection of 4E-BP1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA

4E-BP1 Antibody (P-1): sc-9977

4E-BP1 Antibody (P-1) is rated 4.5 out of 5 by 33.
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Datasheets

    • 4E-BP1 Antibody (P-1) is a mouse monoclonal IgG1 (kappa light chain) provided at 200 µg/ml
    • raised against full length 4E-BP1 of human origin
    • 4E-BP1 Antibody (P-1) is recommended for detection of 4E-BP1 of mouse, rat, human and porcine origin by WB, IP, IF, IHC(P) and ELISA
    • 4E-BP1 Antibody (P-1) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • See m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP), our highly recommended recombinant alternative to conventional secondary anti-mouse IgG reagents.
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of 4E-BP1 (P-1): sc-9977.
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Rated 5 out of 5 by from it is very good I use this product for IHC in mouse skin samples,the dilution is 1 :100,the staining is very great!
Date published: 2017-08-04
Rated 4 out of 5 by from Good antibody Need to use at least 1:250 in order to get a specific band.
Date published: 2017-07-23
Rated 5 out of 5 by from great speceficity Monoclonal anti-4E-BP1 used in western blot with great speceficity and very low background. Used in concentration of 1:800.
Date published: 2017-07-23
Rated 5 out of 5 by from Good antibody! I bought this antibody two months ago and tried on mouse brain lysate. The signal is good!
Date published: 2017-07-13
Rated 5 out of 5 by from strong band I loaded 100ug mouse liver protein per lane, used at a concentration of 1:1000 dilution for overnight. Finally, I got a very stronger band at around 20KD.
Date published: 2017-05-28
Rated 5 out of 5 by from Great This is the first time that I used santa cruze antibodies. I ofen the antibody of cell signal or abcam, so I was very worried. Surprisingly the result is very good. I chose the antibody dilution ratio for (1:500) WB
Date published: 2017-04-07
Rated 4 out of 5 by from ok I used 1:500 dilution in WB, I felt the specificity of this antibody was not so good.
Date published: 2017-03-21
Rated 2 out of 5 by from 没能杂到条带 WB没有带, 试了不同的方法, 希望改进。 细胞系是293和hela。
Date published: 2017-03-06
Rated 1 out of 5 by from No signal No signal by wb at either 1/500 and 1/100, O/N 4°C in TBST + 10% FBS on 20µg depositis of murine fibroblasts.
Date published: 2017-02-28
Rated 4 out of 5 by from sc-9977 Not too easy to do for WB, so hope you to improve.
Date published: 2017-02-20
Rated 5 out of 5 by from 4 e - bp1 transfection 293 t cell lysate Antibodies in a clean, circular band from bank in mouse 4 e - bp1 transfection 293 t cell lysate
Date published: 2017-02-11
Rated 5 out of 5 by from High specific The background is clean.Our experiment was a big success with the product
Date published: 2017-02-11
Rated 5 out of 5 by from Favorite antibody. We use this antibody both IHC (P) or WB to get the perfect results of the experiment, our laboratory people like to use this antibody.
Date published: 2017-02-05
Rated 5 out of 5 by from Strong western and IHC Great WB and IHC results; much stronger antibody than polyclonal alternatives
Date published: 2017-01-14
Rated 5 out of 5 by from Excellent IHC(P) results The 4E-BP1 Antibody (P-1): sc-9977 shows strong staining pattern localized to the cytoplasm when working with formalin fixed, paraffin-embedded tissue sections for IHC.
Date published: 2017-01-10
Rated 5 out of 5 by from Antibodies to clean and clear Antibodies are clean, clear and get the bank in the mouse 4 e - bp1 transfection combination of 293 t cell lysate
Date published: 2017-01-08
Rated 5 out of 5 by from this has worked very well in WB this has worked very well in WB. it is predictable and reliable.
Date published: 2016-07-29
Rated 5 out of 5 by from Clean Clean, round band obtained in WB in mouse 4E-BP1 transfected 293T whole cell lysate. -SCBT QC
Date published: 2015-06-06
Rated 5 out of 5 by from Published IHC data for use in human tumor tissues Published IHC data for use in human tumor tissues. -SCBT Publication Review
Date published: 2015-05-17
Rated 5 out of 5 by from Published data for WB in human PDAC Published data for WB in human PDAC, GC, human embryonic pancreas derived cells and human chronic leukemia cells. -SCBT Publication Review
Date published: 2015-05-12
Rated 5 out of 5 by from Published data for WB in human thymoma cells (EL4) Published data for WB in human thymoma cells (EL4). -SCBT Publication Review
Date published: 2015-04-13
Rated 5 out of 5 by from Great specificity in WB in A Great specificity in WB in A-431, K-562, NIH/3T3 and KNRK whole cell lysates. -SCBT QC
Date published: 2015-04-06
Rated 5 out of 5 by from Published data for IP and WB in rat H9C2 cells Published data for IP and WB in rat H9C2 cells. -SCBT Publication Review
Date published: 2015-04-02
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmic Produced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human pancreas tissue. -SCBT QC
Date published: 2015-03-16
Rated 5 out of 5 by from Published data for WB in human ovarian cancer Published data for WB in human ovarian cancer cell line SKOV-3 and prostatic cancer cell line PC-3. SCBT Publication Review
Date published: 2015-03-08
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